Functional expression and characterization of the human ABCG1 and ABCG4 proteins: indications for heterodimerization

• Published in: Biochemical and biophysical research communications Vol. 320; no. 3; pp. 860 - 867
• Main Authors: Cserepes, Judit, Szentpétery, Zsófia, Seres, László, Özvegy-Laczka, Csilla, Langmann, Thomas, Schmitz, Gerd, Glavinas, Hristos, Klein, Izabella, Homolya, László, Váradi, András, Sarkadi, Balázs, Elkind, N.Barry
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.07.2004
• Subjects: ABC half-transporter; ABCG1; ABCG4; Adenosine Triphosphatases - metabolism; Animals; ATP Binding Cassette Transporter, Sub-Family G; ATP Binding Cassette Transporter, Sub-Family G, Member 1; ATP-Binding Cassette Transporters - metabolism; Cell Line; Cell Membrane - drug effects; Cell Membrane - metabolism; Dimerization; Dominant negative; Drosophila - drug effects; Drosophila - metabolism; Drug-stimulated ATPase activity; Heterodimer; Humans; Protein Binding; Recombinant Proteins - metabolism; Sf9 cells; Vanadates - pharmacology; Dominant negative; ABCG4; Heterodimer; ABCG1; Drug-stimulated ATPase activity; ABC half-transporter; Sf9 cells

The closely related human ABC half-transporters, ABCG1 and ABCG4, have been suggested to play an important role in cellular lipid/sterol regulation but no experimental data for their expression or function are available. We expressed ABCG1 and ABCG4 and their catalytic site mutant variants in insect...