Cloning of cDNAs encoding neurotoxic peptides from the spider Phoneutria nigriventer

• Published in: Toxicon (Oxford) Vol. 36; no. 12; pp. 1843 - 1850
• Main Authors: Kalapothakis, Evanguedes, Penaforte, Claudia L, Beirão, Paulo S.L, Romano-Silva, Marco A, Cruz, Jader S, Prado, Marco A.M, Guimarães, Pedro E.M, Gomez, Marcus V, Prado, Vania F
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.12.1998; Elsevier Science
• Subjects: Amino Acid Sequence; Animal poisons toxicology. Antivenoms; Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; Cloning, Molecular - methods; DNA, Complementary - chemistry; Gene Library; Medical sciences; Molecular Sequence Data; Neurotoxins - chemistry; Phoneutria nigriventer; Protein Sorting Signals - chemistry; Recombinant Proteins; Spider Venoms - genetics; Spiders; Toxicology; Polymerase chain reaction; Arachnida; Complementary DNA; Peptides; Arthropoda; Venom; Neurotoxin; Animal origin; Invertebrata; Aminoacid sequence; Structural analysis

A cDNA library made from venom glands of the spider Phoneutria nigriventer was constructed and used to clone neurotoxic peptides. A cDNA of about 360 nucleotides encoding the precursor for the toxin Tx2-1 active on mammals has been isolated. The deduced amino acid sequence for the mature polypeptide...