Influence of growth regulators (IBA, BA) on anatomical and morphological changes in bromeliads in in vitro culture

• Published in: Acta Societatis Botanicorum Poloniae Vol. 65; no. 3-4; pp. 213 - 219
• Main Authors: Galek, R. (University of Agriculture, Wroclaw (Poland)), Kukulczanka, K. (University of Wroclaw (Poland). Botanical Garden)
• Format: Journal Article
• Language: English
• Published: Warsaw Polish Botanical Society 01.01.1996
• Subjects: 6-BENZILADENINA; 6-BENZYLADENINE; adventitious shoots; AIB; ANATOMIA DE LA PLANTA; ANATOMIE VEGETALE; anatomy; BROMELIACEAE; Cell culture; CULTIVO IN VITRO; CULTURE IN VITRO; cytokinins; Elongation; Epidermis; EXPERIMENTACION IN VITRO; EXPERIMENTATION IN VITRO; FEUILLE; growth and development; Growth regulators; Guzmania; HOJAS; IBA; in vitro; in vitro culture; indole butyric acid; Leaves; MEDIO DE CULTIVO; meristems; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; Morphology; Parenchyma; pith; Propagation; RM medium; Shoots; Species; Stomata; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; TILLANDSIA; TRASTORNOS DEL CRECIMIENTO; TROUBLE DE LA CROISSANCE
• ISSN: 0001-6977; 2083-9480
• DOI: 10.5586/asbp.1996.034

The subject of study were Tillandsia coronata and Guzmania monostachya. The material has been obtained by means of in vitro propagation. The plants were grown for 18 weeks on various kinds of media. Morphological changes were recorded in both species subjected to action of growth regulators. The changes in plant habit were linked with anatomic build. Leaves of plant grown on media containing cytokinin BA were built of a higher number of cell layers of assimilation parenchyma. In plants grown on media with addition of cytokinin the size of stomatal cells was smaller and was accompanied by analogous changes in size of epidermis cells proper. The stomata of plants of both species grown on media with addition of auxin were bigger