Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells

• Published in: Actanaturae Vol. 6; no. 1; pp. 45 - 53
• Main Authors: Muchkaeva, I A, Dashinimaev, E B, Artyuhov, A S, Myagkova, E P, Vorotelyak, E A, Yegorov, Y Y, Vishnyakova, K S, Kravchenko, I E, Chumakov, P M, Terskikh, V V, Vasiliev, A V
• Format: Journal Article
• Language: English
• Published: Russia (Federation) A.I. Gordeyev 01.01.2014
• Subjects: reprogramming; Hair follicle dermal papilla (DP) cells; induced pluripotent stem (iPS) cells
• ISSN: 2075-8251
• DOI: 10.32607/20758251-2014-6-1-45-53

Dermal papilla (DP) cells are unique regional stem cells of the skin that induce formation of a hair follicle and its regeneration cycle. DP are multipotent stem cells; therefore we supposed that the efficiency of DPC reprogramming could exceed that of dermal fibroblasts reprogramming. We generated induced pluripotent stem cells from human DP cells using lentiviral transfection with Oct4, Sox2, Klf4, and c-Myc, and cultivation of cells both in a medium supplemented with valproic acid and at a physiological level of oxygen (5%). The efficiency of DP cells reprogramming was ~0.03%, while the efficiency of dermal fibroblast reprogramming under the same conditions was ~0.01%. Therefore, we demonstrated the suitability of DP cells as an alternative source of iPS cells.