Investigating the Role of Antigen Orientation on the Immune Response Elicited by Neisseria meningitidis Factor H Binding Protein on GMMA

• Published in: Vaccines (Basel) Vol. 10; no. 8; p. 1182
• Main Authors: Alfini, Renzo, Brunelli, Brunella, Bartolini, Erika, Carducci, Martina, Luzzi, Enrico, Ferlicca, Francesca, Buccato, Scilla, Galli, Barbara, Lo Surdo, Paola, Scarselli, Maria, Romagnoli, Giacomo, Cartocci, Elena, Maione, Domenico, Savino, Silvana, Necchi, Francesca, Delany, Isabel, Micoli, Francesca
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.07.2022; MDPI
• Subjects: Antibodies; Antigens; Bacteria; Bactericidal activity; Conjugates; Conjugation; Genetic modification; GMMA; Gram-negative bacteria; Immune response; Immune system; Immunogenicity; Membrane vesicles; N-Terminus; Neisseria meningitidis; Orientation; outer membrane vesicles; Protein arrays; Proteins; Serum bactericidal activity; Vaccines; Neisseria meningitidis; GMMA; outer membrane vesicles; conjugation

GMMA are outer membrane vesicles (OMVs) released from Gram-negative bacteria genetically modified to enhance OMVs formation that have been shown to be optimal systems to enhance immunogenicity of protein antigens. Here, we selected factor H binding protein (fHbp) and used the conjugation chemistry a...