Developmental shift of synaptic vesicle protein 2 from axons to terminals in the primary visual projection of the hamster

• Published in: Neuroscience Vol. 77; no. 4; pp. 1225 - 1236
• Main Authors: Confaloni, A, Lyckman, A.W, Moya, K.L
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.1997; Elsevier
• Subjects: Animals; Antibodies, Monoclonal; axon elongation; Biological and medical sciences; Cells, Cultured; Cricetinae; development; Development. Senescence. Regeneration. Transplantation; Eye Enucleation; Fundamental and applied biological sciences. Psychology; Geniculate Bodies - cytology; Geniculate Bodies - growth & development; Immunohistochemistry; Membrane Glycoproteins - analysis; Membrane Glycoproteins - immunology; Membrane Glycoproteins - metabolism; Mesocricetus; Nerve Tissue Proteins - analysis; Nerve Tissue Proteins - immunology; Nerve Tissue Proteins - metabolism; Neurotransmitter Agents - analysis; Neurotransmitter Agents - immunology; Neurotransmitter Agents - metabolism; Presynaptic Terminals - chemistry; Presynaptic Terminals - metabolism; Retina - chemistry; Retina - cytology; Retina - growth & development; retinal axon; Superior Colliculi - cytology; Superior Colliculi - growth & development; synaptic vesicle protein 2; synaptogenesis; Vertebrates: nervous system and sense organs; Visual Pathways; GAP, growth-associated protein; SGS, stratum griseum superficiale; NHS, normal horse serum; EGTA, ethyleneglycolbis(aminoethylether)tetra-acetate; retinal axon; OT, optic tract; P, postnatal day; LGN, lateral geniculate nucleus; PBS, phosphate-buffered saline; PVDF, polyvinylidene difluoride; TH, total homogenate; SO, stratum opticum; SDS, sodium dodecyl sulphate; CY, cytosolic fraction; synaptic vesicle protein 2; development; BSA, bovine serum albumin; SC, superior colliculus; SV2, synaptic vesicle protein 2; PBSTN, PBS containing Triton X-100 and NaN 3; axon elongation; MAb, monoclonal antibody; TP, total particulate; TBST, Tris buffered saline with Tween-20; synaptogenesis; Synaptogenesis; Rodentia; Synaptic vesicle; Axon; Projection; Retina; Postnatal development; Colliculus superior; Proteins; Vertebrata; Mammalia; Lateral geniculate body; Visual pathway; Hamster

Synaptic vesicle protein 2 is an integral synaptic vesicle membrane glycoprotein which is present in all synapses for which it has been examined. We used an anti-synaptic vesicle protein 2 monoclonal antibody to examine synaptic vesicle protein 2 localization in the developing hamster retinofugal pa...