A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level
LIM kinase 1 (LIMK1), a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analy...
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Published in | Neuroscience bulletin Vol. 29; no. 3; pp. 348 - 354 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
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Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
01.06.2013
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Abstract | LIM kinase 1 (LIMK1), a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI (8.0) software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt +884 to +966 in the human LIMK1 3' untranslated region (UTR) was highly conserved in the mouse Limkl 3' UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3' UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3'-UTR mutant and neuroloqical disorders. |
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AbstractList | LIM kinase 1 (LIMK1), a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI (8.0) software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt +884 to +966 in the human LIMK1 3' untranslated region (UTR) was highly conserved in the mouse Limk1 3' UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only ~60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to ~50% of that in cells transfected with pGL4-hLIMK1-3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3' UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3'-UTR mutant and neurological disorders. LIM kinase 1 (LIMK1), a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI (8.0) software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt +884 to +966 in the human LIMK1 3′ untranslated region (UTR) was highly conserved in the mouse Limk1 3′ UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only ∼60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to ∼50% of that in cells transfected with pGL4-hLIMK1-3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3′ UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3′-UTR mutant and neurological disorders. LIM kinase 1 (LIMK1), a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI (8.0) software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt +884 to +966 in the human LIMK1 3' untranslated region (UTR) was highly conserved in the mouse Limkl 3' UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3' UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3'-UTR mutant and neuroloqical disorders. |
Author | Guang-Fei Deng Shu-Jing Liu1 Xun-Sha Sun Wei-Wen Sun Qi-Hua Zhao Wei-Ping Liao Yong-Hong Yi Yue-Sheng Long |
AuthorAffiliation | Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China,Institute of Neuroscience, and the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China Key Laboratory for Major Obstetric Diseases of Guangdong Province and Key Laboratory of Reproduction and Genetics ofGuangdong Higher Education Institutes, Guangzhou Medical University, Guangzhou 510150, China |
Author_xml | – sequence: 1 givenname: Guang-Fei surname: Deng fullname: Deng, Guang-Fei organization: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience, Hospital of Guangzhou Medical University – sequence: 2 givenname: Shu-Jing surname: Liu fullname: Liu, Shu-Jing organization: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience, Hospital of Guangzhou Medical University – sequence: 3 givenname: Xun-Sha surname: Sun fullname: Sun, Xun-Sha organization: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience, Hospital of Guangzhou Medical University – sequence: 4 givenname: Wei-Wen surname: Sun fullname: Sun, Wei-Wen organization: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience, Hospital of Guangzhou Medical University – sequence: 5 givenname: Qi-Hua surname: Zhao fullname: Zhao, Qi-Hua organization: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience, Hospital of Guangzhou Medical University – sequence: 6 givenname: Wei-Ping surname: Liao fullname: Liao, Wei-Ping organization: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience, Hospital of Guangzhou Medical University – sequence: 7 givenname: Yong-Hong surname: Yi fullname: Yi, Yong-Hong email: yyh168@sina.com organization: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience, Hospital of Guangzhou Medical University – sequence: 8 givenname: Yue-Sheng surname: Long fullname: Long, Yue-Sheng email: longyuesheng@gzhmc.edu.cn organization: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience, Hospital of Guangzhou Medical University, Key Laboratory for Major Obstetric Diseases of Guangdong Province and Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou Medical University |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23700283$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1038/ng0796-272 10.1074/jbc.M207007200 10.1016/S0896-6273(02)00758-4 10.1007/s12031-011-9492-8 10.1016/S0896-6273(03)00633-0 10.1038/31735 10.1111/j.1471-4159.2010.07016.x 10.1038/nature03441 10.1016/j.neuropsychologia.2005.08.007 10.1038/nature05295 10.1016/0955-0674(95)80094-8 10.1016/S0169-328X(96)00257-4 10.1042/BC20080104 10.1038/nature04367 10.1016/j.cub.2007.09.037 10.1074/jbc.M005471200 10.1016/j.pnsc.2009.09.004 10.1016/j.tcb.2009.06.001 10.1016/j.molcel.2010.09.028 10.1016/S0378-1119(01)00350-X 10.1093/hmg/ddl096 10.1038/sj.emboj.7601977 10.1038/nrg1125 10.1002/jnr.21790 10.4161/rna.20231 10.1128/MMBR.63.2.405-445.1999 |
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Copyright | Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2013 |
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DocumentTitleAlternate | A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level |
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Notes | LIM kinase 1 (LIMK1), a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI (8.0) software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt +884 to +966 in the human LIMK1 3' untranslated region (UTR) was highly conserved in the mouse Limkl 3' UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3' UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3'-UTR mutant and neuroloqical disorders. LIM kinase 1;region; post-transcriptsecondary structurethree-prime untranslatedonal regulation; RNA 31-1975/R ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
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References | Kilav, Silver, Naveh-Many (CR27) 2001; 276 Yang, Higuchi, Ohashi, Nagata, Wada, Kangawa (CR1) 1998; 393 Zhao, Hyman, Moore (CR10) 1999; 63 Kuersten, Goodwin (CR11) 2003; 4 Deng, Qin, Sun, Kuang, Su, Zhao (CR18) 2011; 44 Long, Shi, Liao (CR17) 2009; 19 Fukuchi, Tsuda (CR24) 2010; 115 Matoulkova, Michalova, Vojtesek, Hrstka (CR21) 2012; 9 Akagawa, Tajima, Sakamoto, Krischek, Yoneyama, Kasuya (CR9) 2006; 15 Proschel, Blouin, Gutowski, Ludwig, Noble (CR4) 1995; 11 Guhaniyogi, Brewer (CR26) 2001; 265 Meng, Zhang, Tregoubov, Janus, Cruz, Jackson (CR3) 2002; 35 Gray, Karmiloff-Smith, Funnell, Tassabehji (CR7) 2006; 44 Shyu, Wilkinson, van Hoof (CR12) 2008; 27 Dent, Gertler (CR2) 2003; 40 Tassabehji, Metcalfe, Fergusson, Carette, Dore, Donnai (CR6) 1996; 13 Spriggs, Bushell, Willis (CR13) 2010; 40 Mori, Okano, Mizuno, Tohyama, Wanaka (CR5) 1997; 45 Long, Zhao, Su, Cai, Zeng, Shi (CR19) 2008; 86 Andreassi, Riccio (CR14) 2009; 19 Pennacchio, Ahituv, Moses, Prabhakar, Nobrega, Shoukry (CR22) 2006; 444 Karmiloff-Smith (CR8) 2007; 17 Chatterjee, Pal (CR15) 2009; 101 Decker, Parker (CR20) 1995; 7 Xie, Lu, Kulbokas, Golub, Mootha, Lindblad-Toh (CR23) 2005; 434 Duquette, Laneuville (CR25) 2002; 277 Schratt, Tuebing, Nigh, Kane, Sabatini, Kiebler (CR16) 2006; 439 14556705 - Neuron. 2003 Oct 9;40(2):209-27 12123613 - Neuron. 2002 Jul 3;35(1):121-33 10357856 - Microbiol Mol Biol Rev. 1999 Jun;63(2):405-45 17086198 - Nature. 2006 Nov 23;444(7118):499-502 12237309 - J Biol Chem. 2002 Nov 22;277(47):44631-7 7662369 - Curr Opin Cell Biol. 1995 Jun;7(3):386-92 18256698 - EMBO J. 2008 Feb 6;27(3):471-81 7478547 - Oncogene. 1995 Oct 5;11(7):1271-81 8673124 - Nat Genet. 1996 Jul;13(3):272-3 20874756 - J Neurochem. 2010 Dec;115(5):1222-33 22614827 - RNA Biol. 2012 May;9(5):563-76 11118432 - J Biol Chem. 2001 Mar 23;276(12):8727-33 21271300 - J Mol Neurosci. 2011 Jun;44(2):115-21 9655398 - Nature. 1998 Jun 25;393(6687):809-12 19716303 - Trends Cell Biol. 2009 Sep;19(9):465-74 19275763 - Biol Cell. 2009 May;101(5):251-62 12897774 - Nat Rev Genet. 2003 Aug;4(8):626-37 9149099 - Brain Res Mol Brain Res. 1997 May;45(2):247-54 15735639 - Nature. 2005 Mar 17;434(7031):338-45 16611674 - Hum Mol Genet. 2006 May 15;15(10):1722-34 20965418 - Mol Cell. 2010 Oct 22;40(2):228-37 18655196 - J Neurosci Res. 2008 Nov 15;86(15):3375-81 18088580 - Curr Biol. 2007 Dec 18;17(24):R1035-6 16421561 - Nature. 2006 Jan 19;439(7074):283-9 16216290 - Neuropsychologia. 2006;44(5):679-85 11255003 - Gene. 2001 Mar 7;265(1-2):11-23 H Akagawa (1341_CR9) 2006; 15 YS Long (1341_CR19) 2008; 86 J Guhaniyogi (1341_CR26) 2001; 265 EW Dent (1341_CR2) 2003; 40 Y Meng (1341_CR3) 2002; 35 C Andreassi (1341_CR14) 2009; 19 CJ Decker (1341_CR20) 1995; 7 N Yang (1341_CR1) 1998; 393 X Xie (1341_CR23) 2005; 434 M Fukuchi (1341_CR24) 2010; 115 S Chatterjee (1341_CR15) 2009; 101 M Duquette (1341_CR25) 2002; 277 YS Long (1341_CR17) 2009; 19 V Gray (1341_CR7) 2006; 44 C Proschel (1341_CR4) 1995; 11 M Tassabehji (1341_CR6) 1996; 13 S Kuersten (1341_CR11) 2003; 4 R Kilav (1341_CR27) 2001; 276 T Mori (1341_CR5) 1997; 45 KA Spriggs (1341_CR13) 2010; 40 J Zhao (1341_CR10) 1999; 63 AB Shyu (1341_CR12) 2008; 27 GF Deng (1341_CR18) 2011; 44 E Matoulkova (1341_CR21) 2012; 9 GM Schratt (1341_CR16) 2006; 439 A Karmiloff-Smith (1341_CR8) 2007; 17 LA Pennacchio (1341_CR22) 2006; 444 |
References_xml | – volume: 13 start-page: 272 year: 1996 end-page: 273 ident: CR6 article-title: LIM-kinase deleted in Williams syndrome publication-title: Nat Genet doi: 10.1038/ng0796-272 contributor: fullname: Donnai – volume: 277 start-page: 44631 year: 2002 end-page: 44637 ident: CR25 article-title: Translational regulation of prostaglandin endoperoxide H synthase-1 mRNA in megakaryocytic MEG-01 cells. Specific protein binding to a conserved 20-nucleotide CIS element in the 3′-untranslated region publication-title: J Biol Chem doi: 10.1074/jbc.M207007200 contributor: fullname: Laneuville – volume: 35 start-page: 121 year: 2002 end-page: 133 ident: CR3 article-title: Abnormal spine morphology and enhanced LTP in LIMK-1 knockout mice publication-title: Neuron doi: 10.1016/S0896-6273(02)00758-4 contributor: fullname: Jackson – volume: 44 start-page: 115 year: 2011 end-page: 121 ident: CR18 article-title: Promoter analysis of mouse Scn3a gene and regulation of the promoter activity by GC box and CpG methylation publication-title: J Mol Neurosci doi: 10.1007/s12031-011-9492-8 contributor: fullname: Zhao – volume: 40 start-page: 209 year: 2003 end-page: 227 ident: CR2 article-title: Cytoskeletal dynamics and transport in growth cone motility and axon guidance publication-title: Neuron doi: 10.1016/S0896-6273(03)00633-0 contributor: fullname: Gertler – volume: 393 start-page: 809 year: 1998 end-page: 812 ident: CR1 article-title: Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization publication-title: Nature doi: 10.1038/31735 contributor: fullname: Kangawa – volume: 115 start-page: 1222 year: 2010 end-page: 1233 ident: CR24 article-title: Involvement of the 3′-untranslated region of the brain-derived neurotrophic factor gene in activity-dependent mRNA stabilization publication-title: J Neurochem doi: 10.1111/j.1471-4159.2010.07016.x contributor: fullname: Tsuda – volume: 434 start-page: 338 year: 2005 end-page: 345 ident: CR23 article-title: Systematic discovery of regulatory motifs in human promoters and 3′ UTRs by comparison of several mammals publication-title: Nature doi: 10.1038/nature03441 contributor: fullname: Lindblad-Toh – volume: 44 start-page: 679 year: 2006 end-page: 685 ident: CR7 article-title: In-depth analysis of spatial cognition in Williams syndrome: A critical assessment of the role of the LIMK1 gene publication-title: Neuropsychologia doi: 10.1016/j.neuropsychologia.2005.08.007 contributor: fullname: Tassabehji – volume: 11 start-page: 1271 year: 1995 end-page: 1281 ident: CR4 article-title: Limk1 is predominantly expressed in neural tissues and phosphorylates serine, threonine and tyrosine residues publication-title: Oncogene contributor: fullname: Noble – volume: 444 start-page: 499 year: 2006 end-page: 502 ident: CR22 article-title: enhancer analysis of human conserved non-coding sequences publication-title: Nature doi: 10.1038/nature05295 contributor: fullname: Shoukry – volume: 7 start-page: 386 year: 1995 end-page: 392 ident: CR20 article-title: Diversity of cytoplasmic functions for the 3′ untranslated region of eukaryotic transcripts publication-title: Curr Opin Cell Biol doi: 10.1016/0955-0674(95)80094-8 contributor: fullname: Parker – volume: 45 start-page: 247 year: 1997 end-page: 254 ident: CR5 article-title: Comparison of tissue distribution of two novel serine/threonine kinase genes containing the LIM motif (LIMK-1 and LIMK-2) in the developing rat publication-title: Brain Res Mol Brain Res doi: 10.1016/S0169-328X(96)00257-4 contributor: fullname: Wanaka – volume: 101 start-page: 251 year: 2009 end-page: 262 ident: CR15 article-title: Role of 5′- and 3′-untranslated regions of mRNAs in human diseases publication-title: Biol Cell doi: 10.1042/BC20080104 contributor: fullname: Pal – volume: 439 start-page: 283 year: 2006 end-page: 289 ident: CR16 article-title: A brain-specific microRNA regulates dendritic spine development publication-title: Nature doi: 10.1038/nature04367 contributor: fullname: Kiebler – volume: 63 start-page: 405 year: 1999 end-page: 445 ident: CR10 article-title: Formation of mRNA 3′ ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis publication-title: Microbiol Mol Biol Rev contributor: fullname: Moore – volume: 17 start-page: R1035 year: 2007 end-page: 1036 ident: CR8 article-title: Williams syndrome publication-title: Curr Biol doi: 10.1016/j.cub.2007.09.037 contributor: fullname: Karmiloff-Smith – volume: 276 start-page: 8727 year: 2001 end-page: 8733 ident: CR27 article-title: A conserved cis-acting element in the parathyroid hormone 3′-untranslated region is sufficient for regulation of RNA stability by calcium and phosphate publication-title: J Biol Chem doi: 10.1074/jbc.M005471200 contributor: fullname: Naveh-Many – volume: 19 start-page: 1675 year: 2009 end-page: 1681 ident: CR17 article-title: Conservation-based prediction of the trasncription regulatory region of the SCN1A gene publication-title: Prog Nat Sci doi: 10.1016/j.pnsc.2009.09.004 contributor: fullname: Liao – volume: 19 start-page: 465 year: 2009 end-page: 474 ident: CR14 article-title: To localize or not to localize: mRNA fate is in 3′UTR ends publication-title: Trends Cell Biol doi: 10.1016/j.tcb.2009.06.001 contributor: fullname: Riccio – volume: 40 start-page: 228 year: 2010 end-page: 237 ident: CR13 article-title: Translational regulation of gene expression during conditions of cell stress publication-title: Mol Cell doi: 10.1016/j.molcel.2010.09.028 contributor: fullname: Willis – volume: 265 start-page: 11 year: 2001 end-page: 23 ident: CR26 article-title: Regulation of mRNA stability in mammalian cells publication-title: Gene doi: 10.1016/S0378-1119(01)00350-X contributor: fullname: Brewer – volume: 15 start-page: 1722 year: 2006 end-page: 1734 ident: CR9 article-title: A haplotype spanning two genes, ELN and LIMK1, decreases their transcripts and confers susceptibility to intracranial aneurysms publication-title: Hum Mol Genet doi: 10.1093/hmg/ddl096 contributor: fullname: Kasuya – volume: 27 start-page: 471 year: 2008 end-page: 481 ident: CR12 article-title: Messenger RNA regulation: to translate or to degrade publication-title: EMBO J doi: 10.1038/sj.emboj.7601977 contributor: fullname: van Hoof – volume: 4 start-page: 626 year: 2003 end-page: 637 ident: CR11 article-title: The power of the 3′ UTR: translational control and development publication-title: Nat Rev Genet doi: 10.1038/nrg1125 contributor: fullname: Goodwin – volume: 86 start-page: 3375 year: 2008 end-page: 3381 ident: CR19 article-title: Identification of the promoter region and the 5′-untranslated exons of the human voltage-gated sodium channel Na(v)1.1 gene (SCN1A) and enhancement of gene expression by the 5′-untranslated exons publication-title: J Neurosci Res doi: 10.1002/jnr.21790 contributor: fullname: Shi – volume: 9 start-page: 563 year: 2012 end-page: 576 ident: CR21 article-title: The role of the 3′ untranslated region in post-transcriptional regulation of protein expression in mammalian cells publication-title: RNA Biol doi: 10.4161/rna.20231 contributor: fullname: Hrstka – volume: 45 start-page: 247 year: 1997 ident: 1341_CR5 publication-title: Brain Res Mol Brain Res doi: 10.1016/S0169-328X(96)00257-4 contributor: fullname: T Mori – volume: 15 start-page: 1722 year: 2006 ident: 1341_CR9 publication-title: Hum Mol Genet doi: 10.1093/hmg/ddl096 contributor: fullname: H Akagawa – volume: 86 start-page: 3375 year: 2008 ident: 1341_CR19 publication-title: J Neurosci Res doi: 10.1002/jnr.21790 contributor: fullname: YS Long – volume: 276 start-page: 8727 year: 2001 ident: 1341_CR27 publication-title: J Biol Chem doi: 10.1074/jbc.M005471200 contributor: fullname: R Kilav – volume: 27 start-page: 471 year: 2008 ident: 1341_CR12 publication-title: EMBO J doi: 10.1038/sj.emboj.7601977 contributor: fullname: AB Shyu – volume: 277 start-page: 44631 year: 2002 ident: 1341_CR25 publication-title: J Biol Chem doi: 10.1074/jbc.M207007200 contributor: fullname: M Duquette – volume: 19 start-page: 465 year: 2009 ident: 1341_CR14 publication-title: Trends Cell Biol doi: 10.1016/j.tcb.2009.06.001 contributor: fullname: C Andreassi – volume: 393 start-page: 809 year: 1998 ident: 1341_CR1 publication-title: Nature doi: 10.1038/31735 contributor: fullname: N Yang – volume: 11 start-page: 1271 year: 1995 ident: 1341_CR4 publication-title: Oncogene contributor: fullname: C Proschel – volume: 13 start-page: 272 year: 1996 ident: 1341_CR6 publication-title: Nat Genet doi: 10.1038/ng0796-272 contributor: fullname: M Tassabehji – volume: 40 start-page: 209 year: 2003 ident: 1341_CR2 publication-title: Neuron doi: 10.1016/S0896-6273(03)00633-0 contributor: fullname: EW Dent – volume: 19 start-page: 1675 year: 2009 ident: 1341_CR17 publication-title: Prog Nat Sci doi: 10.1016/j.pnsc.2009.09.004 contributor: fullname: YS Long – volume: 7 start-page: 386 year: 1995 ident: 1341_CR20 publication-title: Curr Opin Cell Biol doi: 10.1016/0955-0674(95)80094-8 contributor: fullname: CJ Decker – volume: 434 start-page: 338 year: 2005 ident: 1341_CR23 publication-title: Nature doi: 10.1038/nature03441 contributor: fullname: X Xie – volume: 4 start-page: 626 year: 2003 ident: 1341_CR11 publication-title: Nat Rev Genet doi: 10.1038/nrg1125 contributor: fullname: S Kuersten – volume: 439 start-page: 283 year: 2006 ident: 1341_CR16 publication-title: Nature doi: 10.1038/nature04367 contributor: fullname: GM Schratt – volume: 40 start-page: 228 year: 2010 ident: 1341_CR13 publication-title: Mol Cell doi: 10.1016/j.molcel.2010.09.028 contributor: fullname: KA Spriggs – volume: 9 start-page: 563 year: 2012 ident: 1341_CR21 publication-title: RNA Biol doi: 10.4161/rna.20231 contributor: fullname: E Matoulkova – volume: 35 start-page: 121 year: 2002 ident: 1341_CR3 publication-title: Neuron doi: 10.1016/S0896-6273(02)00758-4 contributor: fullname: Y Meng – volume: 44 start-page: 115 year: 2011 ident: 1341_CR18 publication-title: J Mol Neurosci doi: 10.1007/s12031-011-9492-8 contributor: fullname: GF Deng – volume: 17 start-page: R1035 year: 2007 ident: 1341_CR8 publication-title: Curr Biol doi: 10.1016/j.cub.2007.09.037 contributor: fullname: A Karmiloff-Smith – volume: 44 start-page: 679 year: 2006 ident: 1341_CR7 publication-title: Neuropsychologia doi: 10.1016/j.neuropsychologia.2005.08.007 contributor: fullname: V Gray – volume: 115 start-page: 1222 year: 2010 ident: 1341_CR24 publication-title: J Neurochem doi: 10.1111/j.1471-4159.2010.07016.x contributor: fullname: M Fukuchi – volume: 265 start-page: 11 year: 2001 ident: 1341_CR26 publication-title: Gene doi: 10.1016/S0378-1119(01)00350-X contributor: fullname: J Guhaniyogi – volume: 101 start-page: 251 year: 2009 ident: 1341_CR15 publication-title: Biol Cell doi: 10.1042/BC20080104 contributor: fullname: S Chatterjee – volume: 63 start-page: 405 year: 1999 ident: 1341_CR10 publication-title: Microbiol Mol Biol Rev doi: 10.1128/MMBR.63.2.405-445.1999 contributor: fullname: J Zhao – volume: 444 start-page: 499 year: 2006 ident: 1341_CR22 publication-title: Nature doi: 10.1038/nature05295 contributor: fullname: LA Pennacchio |
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Snippet | LIM kinase 1 (LIMK1), a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and... LIM kinase 1 (LIMK1), a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and... |
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SubjectTerms | 3' Untranslated Regions - genetics Anatomy Anesthesiology Animals Biomedical and Life Sciences Biomedicine Cell Line Central Nervous System - metabolism Gene Expression Regulation Human Physiology Humans Lim Kinases - genetics Lim Kinases - metabolism Mice mRNA水平 Neurology Neurosciences Original Original Article Pain Medicine Phosphorylation RNA, Messenger - genetics RNA, Messenger - metabolism SH-SY5Y细胞 Transcriptional Activation - genetics Transcriptional Activation - physiology Transfection - methods 丝氨酸;苏氨酸激酶 保守区 基因蛋白 实时定量RT-PCR 转录后水平 非编码区 |
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Title | A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level |
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