Morphological and ultrastructural aspects of the activation of avian medullary bone osteoclasts by parathyroid hormone
The activation of physiologically inactive medullary bone osteoclasts by parathyroid hormone (PTH) was examined using light and electron microscopy and histomorphometric methods. Medullary bone osteoclasts are functionally inactive during the avian egg-laying cycle when an egg shell is not being cal...
Saved in:
Published in | The Anatomical record Vol. 208; no. 2; pp. 223 - 231 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.02.1984
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | The activation of physiologically inactive medullary bone osteoclasts by parathyroid hormone (PTH) was examined using light and electron microscopy and histomorphometric methods. Medullary bone osteoclasts are functionally inactive during the avian egg-laying cycle when an egg shell is not being calcified in the shell gland. Japanese quail hens were given 0.5 IU/g PTH and the medullary bone osteoclasts were examined up to 90 min later. Administration of PTH results in rapid changes in osteoclast morphology and ultrastructure. Within 10 min ectoplasmic regions containing condensed-appearing material are evident in areas of the cell adjacent to bone surfaces. In tannic acid-fixed specimens, these ectoplasmic regions contain bundles of filaments extending perpendicularly from the osteoclast plasma membrane into the cytoplasm. It is in these areas that ruffled border development is initiated. Even at 10 min after PTH administration, mineral crystals are seen between the developing cell surface invaginations and folds. By 15 min after PTH administration, ruffled borders have appeared next to bone surfaces. The rapid development of ruffled borders on medullary bone osteoclasts after PTH is confirmed by electron microscope histomorphometry. By 30 min after PTH administration, ruffled borders are well developed and large endocytic vacuoles are beginning to appear in the osteoclast cytoplasm. Light microscope histomorphometric measurements indicate that osteoclasts are also increasing in size and spreading along bone surfaces with time after PTH administration. This study provides a morphologic and ultrastructural description of osteoclast activation by PTH. The results indicate that osteoclasts may effect rapid changes in bone resorption and mineral metabolism due to exogenous PTH in hens. |
---|---|
AbstractList | The activation of physiologically inactive medullary bone osteoclasts by parathyroid hormone (PTH) was examined using light and electron microscopy and histomorphometric methods. Medullary bone osteoclasts are functionally inactive during the avian egg-laying cycle when an egg shell is not being calcified in the shell gland. Japanese quail hens were given 0.5 IU/g PTH and the medullary bone osteoclasts were examined up to 90 min later. Administration of PTH results in rapid changes in osteoclast morphology and ultrastructure. Within 10 min ectoplasmic regions containing condensed-appearing material are evident in areas of the cell adjacent to bone surfaces. In tannic acid-fixed specimens, these ectoplasmic regions contain bundles of filaments extending perpendicularly from the osteoclast plasma membrane into the cytoplasm. It is in these areas that ruffled border development is initiated. Even at 10 min after PTH administration, mineral crystals are seen between the developing cell surface invaginations and folds. By 15 min after PTH administration, ruffled borders have appeared next to bone surfaces. The rapid development of ruffled borders on medullary bone osteoclasts after PTH is confirmed by electron microscope histomorphometry. By 30 min after PTH administration, ruffled borders are well developed and large endocytic vacuoles are beginning to appear in the osteoclast cytoplasm. Light microscope histomorphometric measurements indicate that osteoclasts are also increasing in size and spreading along bone surfaces with time after PTH administration. This study provides a morphologic and ultrastructural description of osteoclast activation by PTH. The results indicate that osteoclasts may effect rapid changes in bone resorption and mineral metabolism due to exogenous PTH in hens. |
Author | Bowman, B.M Myers, R.L Miller, S.C |
Author_xml | – sequence: 1 fullname: Miller, S.C – sequence: 2 fullname: Bowman, B.M – sequence: 3 fullname: Myers, R.L |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/6703339$$D View this record in MEDLINE/PubMed |
BookMark | eNotj0tPwzAQhC1UVNrCkSPCfyCwtvPyEVW8pCIOUIlbtHHsJiiNI9up1H-Pq3Ia7cxq9M2SzAY7aEJuGTwwAP6ILqrkUAIHeUEW8SgSYGU2IwsAEAkv8p8rsvT-F4CxNM_nZJ4XIISQC3L4sG5sbW93ncKe4tDQqQ8OfXCTCpM7eX7UKnhqDQ2tpqhCd8DQ2eHk4KHDge51M_U9uiOtIxy1Pmir-ljiaX2kIzoM7dHZrqGtdfv4ck0uDfZe3_zrimxfnr_Xb8nm8_V9_bRJVBrXJBk2mUQlUlWAyVKuSsWFrDVXJhW8TmUGwtSABlFmjBV1rkqdAkKjGqNkzlfk7tw7TnWErEbX7SNm9b8_5vfn3KCtcOc6X22_ODABPBNpDgX_A0qxayY |
CitedBy_id | crossref_primary_10_1136_jcp_38_3_241 crossref_primary_10_1080_02724634_1997_10011027 crossref_primary_10_1359_jbmr_2002_17_9_1667 crossref_primary_10_1007_BF02557836 crossref_primary_10_1002_ar_1092430404 crossref_primary_10_3390_ani11020570 crossref_primary_10_1080_00071669308417602 crossref_primary_10_1002_1096_9896_2000_9999_9999___AID_PATH645_3_0_CO_2_Q crossref_primary_10_1093_jn_117_3_619 crossref_primary_10_3389_fphys_2022_942790 crossref_primary_10_3109_01913129009076137 crossref_primary_10_1002_ar_1092310303 crossref_primary_10_1038_srep06253 crossref_primary_10_1242_jeb_184_1_63 crossref_primary_10_1080_03079459408418991 crossref_primary_10_3109_10409239609110575 crossref_primary_10_1080_03079459308418955 crossref_primary_10_3382_ps_pex059 crossref_primary_10_1210_endo_143_3_8701 crossref_primary_10_1007_BF02509381 crossref_primary_10_1016_S1042_3699_20_30351_4 crossref_primary_10_1007_BF02555968 crossref_primary_10_1111_jeb_13019 crossref_primary_10_1002_aja_1001830102 crossref_primary_10_1002__SICI_1097_0185_199601_244_1_33__AID_AR4_3_0_CO_2_G crossref_primary_10_1016_8756_3282_94_90299_2 crossref_primary_10_1111_j_1469_185X_1993_tb00996_x |
ContentType | Journal Article |
DBID | FBQ CGR CUY CVF ECM EIF NPM |
DOI | 10.1002/ar.1092080209 |
DatabaseName | AGRIS Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Medicine Zoology Biology |
EISSN | 1097-0185 |
EndPage | 231 |
ExternalDocumentID | 6703339 US201302534607 |
Genre | Research Support, U.S. Gov't, Non-P.H.S Research Support, U.S. Gov't, P.H.S Journal Article |
GrantInformation_xml | – fundername: NIDCR NIH HHS grantid: DE-06007 |
GroupedDBID | -~X .55 .GA .GJ .Y3 10A 123 1L6 1OB 1OC 1ZS 23M 24P 31~ 3O- 3SF 3WU 4.4 4ZD 51W 51X 52N 52O 52P 52T 52U 52W 52X 53G 66C 7PT 8-1 8-4 8-5 85S 930 A03 AAEVG AAHHS AANLZ AAXRX AAZKR ABCUV ABHUG ABIJN ABJNI ABOCM ABPPZ ABWRO ACAHQ ACBWZ ACCFJ ACCZN ACFBH ACNCT ACPOU ACSMX ACXBN ACXME ACXQS ADAWD ADBBV ADDAD ADEOM ADIZJ ADMGS ADOZA AEEZP AEIGN AEIMD AEQDE AEQTP AETEA AEUQT AEUYR AFBPY AFDAS AFFNX AFFPM AFGKR AFMIJ AFPWT AFVGU AFZJQ AGHSJ AGJLS AIURR AIWBW AJBDE ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ASPBG ATUGU AVWKF AZFZN BAWUL BDRZF BQCPF BRXPI BY8 CS3 D-F DCZOG DIK DRFUL DRSTM EBS EJD F00 F01 F04 F5P FA8 FBQ G-S GODZA HBH HF~ JPC LATKE LAW LEEKS LH4 LITHE LOXES LP6 LP7 LPU LUTES LYRES M56 MEWTI MRFUL MRSTM MSFUL MSSTM MVM MXFUL MXSTM NEJ OHT OK1 P4D PALCI Q11 QB0 QRW RIWAO ROL RWI RWR SAMSI SUPJJ UB1 WHG WIH WIK WQJ WRC WXSBR WYB X7M XG1 XJT XOL XPP XV2 YCJ YZZ ZGI ZXP ZZTAW ~02 79B AHBTC AITYG CGR CUY CVF ECM EIF HGLYW NPM WIN |
ID | FETCH-LOGICAL-c4209-5ad59ac34c70f542c8c239be2cf432b49503fb0afaa95117b6c8e40a0dcdfc962 |
ISSN | 0003-276X |
IngestDate | Sat Sep 28 07:33:13 EDT 2024 Wed Dec 27 19:32:23 EST 2023 |
IsPeerReviewed | false |
IsScholarly | false |
Issue | 2 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c4209-5ad59ac34c70f542c8c239be2cf432b49503fb0afaa95117b6c8e40a0dcdfc962 |
PMID | 6703339 |
PageCount | 9 |
ParticipantIDs | pubmed_primary_6703339 fao_agris_US201302534607 |
PublicationCentury | 1900 |
PublicationDate | February 1984 |
PublicationDateYYYYMMDD | 1984-02-01 |
PublicationDate_xml | – month: 02 year: 1984 text: February 1984 |
PublicationDecade | 1980 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | The Anatomical record |
PublicationTitleAlternate | Anat Rec |
PublicationYear | 1984 |
SSID | ssj0011466 |
Score | 1.2499285 |
Snippet | The activation of physiologically inactive medullary bone osteoclasts by parathyroid hormone (PTH) was examined using light and electron microscopy and... |
SourceID | pubmed fao |
SourceType | Index Database Publisher |
StartPage | 223 |
SubjectTerms | animal anatomy animal morphology Animals Bone and Bones - drug effects Bone and Bones - ultrastructure Coturnix - anatomy & histology Coturnix - physiology Female Microscopy, Electron Osteoclasts - drug effects Osteoclasts - ultrastructure Oviposition Parathyroid Hormone - pharmacology Quail - physiology |
Title | Morphological and ultrastructural aspects of the activation of avian medullary bone osteoclasts by parathyroid hormone |
URI | https://www.ncbi.nlm.nih.gov/pubmed/6703339 |
Volume | 208 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1baxQxFA5aUXwpWi2tN_LgW5l1NpO55NEVSxG3qO1C8WVJMkkryI6s68r21_fLZWdmVwX1JQyBCTM5X845Sc75DiEvmdaqEgbrm-d1wktWJ6owLClMJRQ3cFGND5A9LU4m_N1FfrGVXbJQA33927yS_5Eq-iBXlyX7D5JtB0UHniFftJAw2r-S8bjBLLXayx2B__i6mMvACev5NKTPpPy-jgRwWQzL1kmUS7e6YQ6xDXXBc6qBx-mSPhoNnxpvwTX11OBXq3nzpT66gn_bzDZih3xN5xk27oF1IBz5tEJs8wzPBu1h7Kj5GQ9dR4MugiCWnjj6NHjfnUMMRcXXocs93ZolrPR1CWFZgjpNHdfrMBTlWetbllY9YLG-9gypx9EQs2AeftHxgTNWzh0TFnOpwqnojFkbYjg5Y_5SNs944dgGbpeVU4DHo4_tJdMwXmWvPzxSsGL8Vxujw-2wstnad3j_4_wB2Y0bB_o6oOAhuWVme-RuKCW62iP3xjFIAp2fG9_5iCw3AEIBELoFEBoBQhtLARDaAcT1eIDQFiDUAYT2AELVivYAQiNAHpPJ8dvzNydJLLSRaI7_S3JZ50LqjOsytTlnutIsE8owbXnGFPbQaWZVKq2UcMiHpSp0ZXgq01rXVouC7ZOdGYY_IFTUwlE2VtzWGQZKlaxsriTH7Jtc5_KQHGAup_ISJmy6KaNDsh8mePotEK1MC5ijLBNP_vzOU3K_w-IzcsdiZZvncBMX6oUXNtrTD-MbNe9p-w |
link.rule.ids | 786 |
linkProvider | FAO Food and Agriculture Organization of the United Nations |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Morphological+and+ultrastructural+aspects+of+the+activation+of+avian+medullary+bone+osteoclasts+by+parathyroid+hormone&rft.jtitle=The+Anatomical+record&rft.au=Miller%2C+S.C&rft.au=Bowman%2C+B.M&rft.au=Myers%2C+R.L&rft.date=1984-02-01&rft.issn=0003-276X&rft.eissn=1097-0185&rft.volume=208&rft.issue=2&rft.spage=223&rft.epage=231&rft_id=info:doi/10.1002%2Far.1092080209&rft.externalDocID=US201302534607 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0003-276X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0003-276X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0003-276X&client=summon |