SNAP-25 is present in a SNARE complex in adrenal chromaffin cells

• Published in: FEBS letters Vol. 351; no. 2; pp. 207 - 210
• Main Authors: Roth, Dagmar, Burgoyne, Robert D.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.09.1994
• Subjects: Adrenal Medulla - chemistry; Amino Acid Sequence; Animals; BoNT, botulinum neurotoxin; Calcium-Binding Proteins; Carrier Proteins - analysis; Cattle; Cell Membrane - chemistry; Exocytosis; Fluorescent Antibody Technique; Membrane Glycoproteins - analysis; Membrane Proteins - analysis; Membrane Proteins - isolation & purification; Microsomes - chemistry; Molecular Sequence Data; N-Ethylmaleimide-sensitive fusion protein (NSF); N-Ethylmaleimide-Sensitive Proteins; Nerve Tissue Proteins - analysis; Nerve Tissue Proteins - isolation & purification; NSF, N-ethylmaleimide-sensitive fusion protein; PNP, post-nuclear pellet; PNS, post-nuclear supernatant; Precipitin Tests; R-SNARE Proteins; Secretion; SNAP; SNAP, soluble NSF attachment protein; SNAP-25, synaptosomal-associated protein 25 kDa; SNARE, SNAP receptor; Synaptosomal-Associated Protein 25; Synaptotagmin; Synaptotagmins; Syntaxin; Vesicular Transport Proteins; NSF, N-ethylmaleimide-sensitive fusion protein; Secretion; PNP, post-nuclear pellet; BoNT, botulinum neurotoxin; SNAP, soluble NSF attachment protein; SNAP-25, synaptosomal-associated protein 25 kDa; Exocytosis; SNAP; Syntaxin; N-Ethylmaleimide-sensitive fusion protein (NSF); SNARE, SNAP receptor; PNS, post-nuclear supernatant; Synaptotagmin
• DOI: 10.1016/0014-5793(94)00833-7
• ISSN: 0014-5793

SNAP-25 (synaptosomal-associated protein 25 kDa) is a target for botulinum neurotoxins A and E, which both inhibit neurotransmitter release, and was recently identified together with syntaxin and synaptobrevin as receptors for NSF and α-SNAP. We show that SNAP-25 was enriched in the microsomal fraction from adrenal medulla, although the level of SNAP-25 in adrenal medullary microsomes was about 20-fold less than in brain microsomes. Immunocytochemistry confirmed the presence of SNAP-25 in cultured chromaffin cells and showed plasma membrane staining. Using immunoprecipitation, we found that SNAP-25 was present in a complex with syntaxin, synaptobrevin, synaptotagmin, NSF, α-SNAP and other unidentified polypeptides. These data indicate that SNAP-25 in chromaffin cells is present in a complex similar to that identified in brain.