Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells

• Published in: Bioengineering (Basel) Vol. 4; no. 3; p. 67
• Main Authors: Sun, Yuxi, Sethu, Palaniappan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.08.2017; MDPI
• Subjects: Adaptation; Beads; Binary mixtures; Bioengineering; Blood; cell separations; Centrifugal force; Centrifugal pumps; Centrifugation; Density; density-gradient centrifugation; Efficiency; Fluids; Fractionation; Granulocytes; Immunoassay; label-free cell separation; microfluidics; Plasma; Polystyrene; Polystyrene resins; Reynolds number; Separation; Silicon dioxide; United States > US; cell separations; label-free cell separation; density-gradient centrifugation; microfluidics

Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to enable fractionation of cells that layer as a nar...