Orientation of Pseudomonas aeruginosa ExsA Monomers Bound to Promoter DNA and Base-Specific Contacts with the PexoT Promoter

• Published in: Journal of Bacteriology Vol. 194; no. 10; pp. 2573 - 2585
• Main Authors: King, Jessica M, Brutinel, Evan D, Marsden, Anne E, Schubot, Florian D, Yahr, Timothy L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.05.2012
• Subjects: ADP Ribose Transferases; Amino Acid Sequence; Bacterial Proteins; Bacteriology; Base Sequence; binding sites; Biological and medical sciences; cysteine; cytosine; DNA; DNA, Bacterial; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; genetics; GTPase-Activating Proteins; guanine; metabolism; Microbiology; Miscellaneous; Models, Molecular; Molecular Sequence Data; Mutagenesis; promoter regions; Promoter Regions, Genetic; Protein Binding; Pseudomonas aeruginosa; Recombinant Fusion Proteins; regulon; sequence homology; Trans-Activators; transcription (genetics); transcription factors; Type III secretion system; Pseudomonadales; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa

ExsA is a transcriptional activator of the Pseudomonas aeruginosa type III secretion system (T3SS) and a member of the AraC/XylS protein family. Each of the 10 ExsA-dependent promoter regions that define the T3SS regulon has two adjacent binding sites for monomeric ExsA. Whereas the promoter-proxima...