Growth of MIN-6 Cells on Salmon Fibrinogen Scaffold Improves Insulin Secretion
The incidence of type I diabetes has been increasing worldwide at an annual rate of approximately 3%. One of the strategies to treat type I diabetes is islet transplantation, in which damaged β-cells are replaced with new islets. To improve β-cells' expansion and pseudoislet formation, studies...
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Published in | Pharmaceutics Vol. 14; no. 5; p. 941 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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26.04.2022
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Abstract | The incidence of type I diabetes has been increasing worldwide at an annual rate of approximately 3%. One of the strategies to treat type I diabetes is islet transplantation, in which damaged β-cells are replaced with new islets. To improve β-cells' expansion and pseudoislet formation, studies are focusing on using extracellular-matrix-resembling substrates. We evaluated the potential of salmon fibrinogen and chitosan electrospun scaffold as cell substrate for cultivating MIN-6 cells. The morphology of cells, insulin secretion and gene expression was evaluated and compared with other substrates (nanofibrous scaffold, microporous scaffold and tissue culture polystyrene). We found that all tested 3D conditions favored the pseudoislet formation of MIN-6 cells. The insulin secretion of MIN-6 cells after stimulation with high-glucose media shows approximately a 9-fold increase compared to the control group when a fibrinogen/chitosan-based electrospun scaffold was used for cultivation. The differences in insulin secretion were corroborated by differences in gene expression. The differences in insulin secretion could probably be attributed to the differences in the mechanical and/or chemical nature of the tested substrates. |
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AbstractList | The incidence of type I diabetes has been increasing worldwide at an annual rate of approximately 3%. One of the strategies to treat type I diabetes is islet transplantation, in which damaged β-cells are replaced with new islets. To improve β-cells’ expansion and pseudoislet formation, studies are focusing on using extracellular-matrix-resembling substrates. We evaluated the potential of salmon fibrinogen and chitosan electrospun scaffold as cell substrate for cultivating MIN-6 cells. The morphology of cells, insulin secretion and gene expression was evaluated and compared with other substrates (nanofibrous scaffold, microporous scaffold and tissue culture polystyrene). We found that all tested 3D conditions favored the pseudoislet formation of MIN-6 cells. The insulin secretion of MIN-6 cells after stimulation with high-glucose media shows approximately a 9-fold increase compared to the control group when a fibrinogen/chitosan-based electrospun scaffold was used for cultivation. The differences in insulin secretion were corroborated by differences in gene expression. The differences in insulin secretion could probably be attributed to the differences in the mechanical and/or chemical nature of the tested substrates. |
Author | Uibo, Raivo Laidmäe, Ivo Aints, Alar |
AuthorAffiliation | 2 Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia 3 Estonian Academy of Sciences, Kohtu 6, 10130 Tallinn, Estonia 1 Department of Immunology, Institute of Biomedicine and Translational Medicine, Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia; alar.aints@ut.ee (A.A.); raivo.uibo@ut.ee (R.U.) |
AuthorAffiliation_xml | – name: 3 Estonian Academy of Sciences, Kohtu 6, 10130 Tallinn, Estonia – name: 1 Department of Immunology, Institute of Biomedicine and Translational Medicine, Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia; alar.aints@ut.ee (A.A.); raivo.uibo@ut.ee (R.U.) – name: 2 Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia |
Author_xml | – sequence: 1 givenname: Ivo orcidid: 0000-0001-5559-5476 surname: Laidmäe fullname: Laidmäe, Ivo organization: Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia – sequence: 2 givenname: Alar orcidid: 0000-0001-7885-2324 surname: Aints fullname: Aints, Alar organization: Department of Immunology, Institute of Biomedicine and Translational Medicine, Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia – sequence: 3 givenname: Raivo surname: Uibo fullname: Uibo, Raivo organization: Estonian Academy of Sciences, Kohtu 6, 10130 Tallinn, Estonia |
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Keywords | salmon fibrinogen 3D cell culturing insulin secretion electrospinning pancreatic islet β-cells |
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Snippet | The incidence of type I diabetes has been increasing worldwide at an annual rate of approximately 3%. One of the strategies to treat type I diabetes is islet... |
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StartPage | 941 |
SubjectTerms | 3D cell culturing Binding sites Cell growth Diabetes electrospinning Extracellular matrix Gene expression Glucose Hydrogels Insulin insulin secretion Morphology pancreatic islet β-cells salmon fibrinogen Tissue engineering Wound healing |
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Title | Growth of MIN-6 Cells on Salmon Fibrinogen Scaffold Improves Insulin Secretion |
URI | https://www.ncbi.nlm.nih.gov/pubmed/35631527 https://www.proquest.com/docview/2670328208/abstract/ https://search.proquest.com/docview/2671271600 https://pubmed.ncbi.nlm.nih.gov/PMC9144899 https://doaj.org/article/059c711623c347f48018f7ac189c9276 |
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