Genetic diversity and phenotypic characterization of Iodobacter limnosediminis associated with skin lesions in freshwater fish

• Published in: Journal of fish diseases Vol. 44; no. 11; pp. 1711 - 1724
• Main Authors: Korkea‐aho, Tiina Liisa, Viljamaa‐Dirks, Satu, Heinikainen, Sirpa, Kuronen, Henry, Tiirola, Marja
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing Ltd 01.11.2021; John Wiley and Sons Inc
• Subjects: Analytical methods; Animal tissues; Aquatic environment; Body organs; Colonies; Electrophoresis; Fish; Fish farms; Fish skin; Freshwater; Freshwater fish; Freshwater fishes; Gel electrophoresis; Gels; Genetic diversity; Genetic variation; Genomes; Inland water environment; Iodobacter limnosediminis; Lesions; Macrophages; Microbiological strains; Microbiota; Organs; Salmo trutta; Salmonids; Saprolegniosis; Skin diseases; Skin lesions; Strains (organisms); Tissue; Ulcers
• DOI: 10.1111/jfd.13490
• ISSN: 0140-7775

The relatively unknown genus Iodobacter sp. has been repeatedly isolated from skin ulcers and saprolegniosis on freshwater fish in Finland, especially farmed salmonids. Genetic characterization verified that all 23 bacterial isolates studied here belonged to the species Iodobacter limnosediminis, previously undescribed from the fish microbiota. Whole‐genome pulsed‐field gel electrophoresis revealed variability between the I. limnosediminis strains, suggesting that they were most likely of environmental origin. Two I. limnosediminis strains caused lesions in 27%–53% of brown trout (Salmo trutta) injected intramuscularly (p ≤ .05). The lesions represented moderate to severe tissue damage, but for most fish, the tissues had been repaired by the end of the experiment through the accumulation of fibrocytes and macrophages at the site of the lesion. I. limnosediminis was reisolated from some lesions and/or internal organs. Phenotypically and biochemically, I. limnosediminis resembles several common bacterial species found in the aquatic environment, as it grows well on several media as whitish medium‐sized colonies, is Gram negative and rod‐shaped. Here, we characterized I. limnosediminis strains with several methods, including MALDI‐TOF. This characterization will help in further investigations into the occurrence and possible involvement of I. limnosediminis in skin lesions of freshwater fish.