Oxidative stress-induced cyclin D1 depletion and its role in cell cycle processing

• Published in: Biochimica et biophysica acta Vol. 1830; no. 11; pp. 5316 - 5325
• Main Authors: Pyo, Chul-Woong, Choi, Joon Hwan, Oh, Sang-Muk, Choi, Sang-Yun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2013
• Subjects: Cell cycle; Cell Cycle - genetics; Cell Cycle - physiology; cell cycle checkpoints; Cell Cycle Checkpoints - genetics; Cell Cycle Checkpoints - physiology; Cell Line; Cell Line, Tumor; Checkpoint Kinase 1; Chk1; Cyclin D1 - genetics; Cyclin D1 - metabolism; cyclins; DNA damage; eIF-2 Kinase - genetics; eIF-2 Kinase - metabolism; G2 Phase - genetics; G2 Phase - physiology; gene silencing; HEK293 Cells; HeLa Cells; Humans; hydrogen peroxide; Hydrogen Peroxide - metabolism; interphase; Oxidative stress; Oxidative Stress - genetics; Oxidative Stress - physiology; Proteasome Endopeptidase Complex - genetics; Proteasome Endopeptidase Complex - metabolism; Proteasome Endopeptidase Complex - physiology; Protein Kinases - genetics; Protein Kinases - metabolism; proteinase inhibitors; Proteolysis; Reactive Oxygen Species - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; translation (genetics); Translational repression; Ubiquitin - metabolism; Oxidative stress; Translational repression; Chk1; Proteolysis; Cell cycle

Cyclin D1 is immediately down-regulated in response to reactive oxygen species (ROS) and implicated in the induction of cell cycle arrest in G2 phase by an unknown mechanism. Either treatment with a protease inhibitor alone or expression of protease-resistant cyclin D1 T286A resulted in only a parti...