Brain blotting: a method to detect multiple DNA copies in specific brain regions

• Published in: The journal of histochemistry and cytochemistry Vol. 36; no. 9; pp. 1191 - 1195
• Main Authors: Hernandez Bronchud, M, Webb, S, Esiri, MM
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Histochemical Soc 01.09.1988; SAGE Publications; Histochemical Society
• Subjects: Animals; Biological and medical sciences; Brain Chemistry; Diverse techniques; DNA - analysis; Frozen Sections; Fundamental and applied biological sciences. Psychology; Humans; Mice; Molecular and cellular biology; Nucleic Acid Hybridization; Protein Biosynthesis; Rats

We developed a method to detect multiple DNA copies (both cellular and viral) in specific brain regions by blotting thick frozen sections onto nylon membranes. This was achieved by "printing" the frozen sections on standard blotting paper immediately after cryotome sectioning and performin...