Steroid Receptor Coactivator-3 Promotes Bladder Cancer Through Upregulation of CXCR4

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 14; no. 6; pp. 3847 - 3850
• Main Authors: Zhang, Yu, Wang, Ji-Hong, Liu, Bin, Qu, Ping-Bao
• Format: Journal Article
• Language: English
• Published: Thailand 01.01.2013
• Subjects: Blotting, Western; Cell Proliferation; Humans; Liver - metabolism; Liver - pathology; Nuclear Receptor Coactivator 3 - antagonists & inhibitors; Nuclear Receptor Coactivator 3 - genetics; Nuclear Receptor Coactivator 3 - metabolism; Real-Time Polymerase Chain Reaction; Receptors, CXCR4 - genetics; Receptors, CXCR4 - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Small Interfering - genetics; Tumor Cells, Cultured; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology
• ISSN: 1513-7368; 2476-762X
• DOI: 10.7314/APJCP.2013.14.6.3847

The three homologous members of the p160 SRC family (SRC-1, SRC-2 and SRC-3) mediate the transcriptional functions of nuclear receptors and other transcription factors, and are the most studied of all the transcriptional co-activators. Recent work has indicated that the SRC-3 gene is subject to amplification and overexpression in various human cancers. Some of the molecular mechanisms responsible for SRC overexpression, along with the mechanisms by which SRC-3 promotes breast and prostate cancer cell proliferation and survival, have been identified. However, the function of SRC-3 in bladder cancer remains poorly understood. In the present study, our results indicate that overexpression of SRC-3 promotes bladder cancer cell proliferation whereas knockdown of SRC-3 results in inhibition. At the molecular level, we further established that CXCR4 is a transcriptional target of SRC-3. Therefore, our study first identified that SRC-3 plays a critical role in the bladder cancer, which may be a target beneficial for its prevention and treatment.