RA-XII, a bicyclic hexapeptidic glucoside isolated from Rubia yunnanensis Diels, exerts antitumor activity by inhibiting protective autophagy and activating Akt-mTOR pathway in colorectal cancer cells

• Published in: Journal of ethnopharmacology Vol. 266; no. NA; p. 113438
• Main Authors: Wang, Jing, Wang, Jia, Li, Ling, Feng, Li, Wang, Yu-Rong, Wang, Zhe, Tan, Ning-Hua
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 10.02.2021
• Subjects: active ingredients; Animals; antineoplastic activity; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Antitumor mechanism; apoptosis; Apoptosis - drug effects; autophagosomes; autophagy; Autophagy - drug effects; cancer therapy; cathepsins; cell cycle checkpoints; Cell Line, Tumor; China; Colorectal cancer; colorectal neoplasms; Colorectal Neoplasms - drug therapy; Cyclopeptide; cytotoxicity; Female; fluorescence; glucosides; HT29 Cells; Humans; membrane potential; Mice; Mice, Inbred BALB C; Mice, Nude; mitochondrial membrane; Oriental traditional medicine; Peptides, Cyclic - isolation & purification; Peptides, Cyclic - pharmacology; plasmids; protein synthesis; Proto-Oncogene Proteins c-akt - metabolism; RA-XII; rheumatoid arthritis; Rubia; Rubia - chemistry; Rubia yunnanensis Diels; Signal Transduction - drug effects; TOR Serine-Threonine Kinases - metabolism; transfection; tuberculosis; Xenograft Model Antitumor Assays; China; ATGs; Antitumor mechanism; DMSO; Colorectal cancer; siRNA; RA-XII; MDC; CQ; Rubia yunnanensis Diels; Cyclopeptide; FBS; mTOR; CTSD; CTSB; JC-1

The roots of Rubia yunnanensis Diels (Chinese name ‘Xiao-Hong-Shen’), a traditional Chinese medicine native to Yunnan province (China), have a long history of use for treating several diseases, such as tuberculosis, rheumatism and cancers. A bicyclic hexapeptidic glucoside named RA-XII was isolated...