Rapid detection of goose hemorrhagic polyomavirus using TaqMan quantitative real-time PCR

• Published in: Molecular and cellular probes Vol. 39; pp. 61 - 64
• Main Authors: Wan, Chunhe, Cheng, Longfei, Fu, Guanghua, Chen, Cuiteng, Liu, Rongchang, Shi, Shaohua, Chen, Hongmei, Fu, Qiuling, Huang, Yu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2018
• Subjects: Animals; Domestic Jinyun Sheldrakes; Geese - virology; Goose hemorrhagic polyomavirus; Polyomavirus - isolation & purification; Real-Time Polymerase Chain Reaction - methods; Reproducibility of Results; Sensitivity; Sensitivity and Specificity; TaqMan real-time PCR; Sensitivity; Goose hemorrhagic polyomavirus; TaqMan real-time PCR; Domestic Jinyun Sheldrakes

Due to low doses of infection, an efficient and sensitive virus detection method is necessary to detect low amounts of goose hemorrhagic polyomavirus (GHPV). In this study, we have developed a TaqMan real-time PCR (qPCR) specific assay for the detection of GHPV. Specificity assay showed no cross-rea...