Effect of different levels of L-carnitine and lysine-methionine on broiler blood parameters

ABSTRACT Objetive. In the present study a completely randomized 3×3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys) and methionine (Met) on the blood concentrations of energy, protein and lipid metabolites of male broiler chickens. Materials and metho...

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Published inRevista MVZ Córdoba Vol. 20; no. 3; pp. 4698 - 4708
Main Authors Hosseintabar, Babak, Dadashbeiki, Mohammad, Bouyeh, Mehrdad, Seidavi, Alireza, Van den Hoven, René, Gamboa, Sandra
Format Journal Article
LanguageEnglish
Published Universidad de Córdoba - Facultad de Medicina Veterinaria y Zootecnia 01.09.2015
Universidad de Cordoba
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Abstract ABSTRACT Objetive. In the present study a completely randomized 3×3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys) and methionine (Met) on the blood concentrations of energy, protein and lipid metabolites of male broiler chickens. Materials and methods. A total of 270 newly hatched male broiler chickens (Ross 308) were randomly assigned to 9 groups (ten broilers per replicate and three replicates per treatment). The control group was fed a basal diet, whereas the treatment groups were fed basal diets supplemented with L-Carnitine (0 mg/kg, 75 mg/kg and 150 mg/kg) and lysine-methionine (0, 15 and 30%) for 42 days. On day 42, one bird was randomly chosen per replication, a blood sample was taken and the blood concentrations of glucose (GLU), uric acid (UAc), triglyceride (TG), VLDL, HDL, LDL, total protein (TP), albumin (Alb) and total cholesterol (TC) were analyzed. Results. Dietary L-carnitine supplementation had a significant effect (p<0.05) on uric acid (UAc), HDL, LDL, and total cholesterol (TC). The birds feed L-carnitine plus Lys and Met presented the highest plasmatic UAc level and the lowest plasmatic TC and LDL level. Moreover, L-carnitine significantly reduced total cholesterol (TC) when compared with both the control group and the birds feed Lys and Met without L-carnitine. Conclusions. A diet with 150 mg/kg L-carnitine plus 15% Lys and Met seems to be enough to sustain low plasmatic TC, LDL and HDL concentrations on male broiler. RESUMEN Objetivo. Se realizó un estudio para determinar el efecto de la suplementación deL-carnitina, lisina (Lys) y metionina (Met) sobre los metabolitos sanguíneos de pollos de engorde. Materiales y métodos. Se utilizaran 270 pollos de la línea Ross 308 de un día de edad y se dividieron en 9 tratamientos en un diseño al azar con arreglo factorial 3x3: tres niveles de L-carnitina (0 mg/kg, 75 mg/kg y 150 mg/kg) y tres de lisina-metionina (0, 15 y 30%) durante 42 días. Cada tratamiento constó de 3 repeticiones con 10 pollos por repetición. El día 42 de edad, se tomaron muestras de sangre de tres aves por tratamiento para cuantificar niveles séricos de glucosa (GLU), ácido úrico (UAc), triglicéridos (TG), VLDL, HDL, LDL, proteínas totales (PT), albúmina (Alb) y colesterol total (TC). Resultados. La suplementación de L-carnitina en la dieta tuvo un efecto significativo (p<0.05) en los niveles de ácido úrico en suero (UAC), HDL, LDL y colesterol total (CT). Las aves alimentadas con L-carnitina más Lys y Met mostraron niveles séricos más altos de UAc y menor TCy LDL. Por otra parte, la L-carnitina redujó significativamente el colesterol total (CT), cuando se comparó con el grupo control y con los pollos alimentados con Lys y Met, sin L-carnitina. Conclusiones. Una dieta con 150 mg/kg de L-carnitina y 15% Lys y Met parece ser suficiente para mantener bajas concentraciones plasmáticas de TC, LDL y HDL en pollos de engorde.
AbstractList Objective. In the present study a completely randomized 3x3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys) and methionine (Met) on the blood concentrations of energy, protein and lipid metabolites of male broiler chickens. Materials and methods. A total of 270 newly hatched male broiler chickens (Ross 308) were randomly assigned to 9 groups (ten broilers per replicate and three replicates per treatment). The control group was fed a basal diet, whereas the treatment groups were fed basal diets supplemented with L-Carnitine (0 mg/kg, 75 mg/kg and 150 mg/kg) and lysine-methionine (0, 15 and 30%) for 42 days. On day 42, one bird was randomly chosen per replication, a blood sample was taken and the blood concentrations of glucose (GLU), uric acid (UAc), triglyceride (TG), VLDL, HDL, LDL, total protein (TP), albumin (Alb) and total cholesterol (TC) were analyzed. Results. Dietary L-carnitine supplementation had a significant effect (p<0.05) on uric acid (UAc), HDL, LDL, and total cholesterol (TC). The birds feed L-carnitine plus Lys and Met presented the highest plasmatic UAc level and the lowest plasmatic TC and LDL level. Moreover, L-carnitine significantly reduced total cholesterol (TC) when compared with both the control group and the birds feed Lys and Met without L-carnitine. Conclusions. A diet with 150 mg/kg L-carnitine plus 15% Lys and Met seems to be enough to sustain low plasmatic TC, LDL and HDL concentrations on male broiler.
Objective. In the present study a completely randomized 3x3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys) and methionine (Met) on the blood concentrations of energy, protein and lipid metabolites of male broiler chickens. Materials and methods. A total of 270 newly hatched male broiler chickens (Ross 308) were randomly assigned to 9 groups (ten broilers per replicate and three replicates per treatment). The control group was fed a basal diet, whereas the treatment groups were fed basal diets supplemented with L-Carnitine (0 mg/kg, 75 mg/kg and 150 mg/kg) and lysine-methionine (0, 15 and 30%) for 42 days. On day 42, one bird was randomly chosen per replication, a blood sample was taken and the blood concentrations of glucose (GLU), uric acid (UAc), triglyceride (TG), VLDL, HDL, LDL, total protein (TP), albumin (Alb) and total cholesterol (TC) were analyzed. Results. Dietary L-carnitine supplementation had a significant effect (p<0.05) on uric acid (UAc), HDL, LDL, and total cholesterol (TC). The birds feed L-carnitine plus Lys and Met presented the highest plasmatic UAc level and the lowest plasmatic TC and LDL level. Moreover, L-carnitine significantly reduced total cholesterol (TC) when compared with both the control group and the birds feed Lys and Met without L-carnitine. Conclusions. A diet with 150 mg/kg L-carnitine plus 15% Lys and Met seems to be enough to sustain low plasmatic TC, LDL and HDL concentrations on male broiler. Objetivo. Se realizó un estudio para determinar el efecto de la suplementación deL-carnitina, lisina (Lys) y metionina (Met) sobre los metabolitos sanguíneos de pollos de engorde. Materiales y métodos. Se utilizaran 270 pollos de la línea Ross 308 de un día de edad y se dividieron en 9 tratamientos en un diseño al azar con arreglo factorial 3x3: tres niveles de L-carnitina (0 mg/kg, 75 mg/kg y 150 mg/kg) y tres de lisina-metionina (0, 15 y 30%) durante 42 días. Cada tratamiento constó de 3 repeticiones con 10 pollos por repetición. El día 42 de edad, se tomaron muestras de sangre de tres aves por tratamiento para cuantificar niveles séricos de glucosa (GLU), ácido úrico (UAc), triglicéridos (TG), VLDL, HDL, LDL, proteínas totales (PT), albúmina (Alb) y colesterol total (TC). Resultados. La suplementación de L-carnitina en la dieta tuvo un efecto significativo (p<0.05) en los niveles de ácido úrico en suero (UAC), HDL, LDL y colesterol total (CT). Las aves alimentadas con L-carnitina más Lys y Met mostraron niveles séricos más altos de UAc y menor TCy LDL. Por otra parte, la L-carnitina redujó significativamente el colesterol total (CT), cuando se comparó con el grupo control y con los pollos alimentados con Lys y Met, sin L-carnitina. Conclusiones. Una dieta con 150 mg/kg de L-carnitina y 15% Lys y Met parece ser suficiente para mantener bajas concentraciones plasmáticas de TC, LDL y HDL en pollos de engorde.
ABSTRACT Objetive. In the present study a completely randomized 3×3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys) and methionine (Met) on the blood concentrations of energy, protein and lipid metabolites of male broiler chickens. Materials and methods. A total of 270 newly hatched male broiler chickens (Ross 308) were randomly assigned to 9 groups (ten broilers per replicate and three replicates per treatment). The control group was fed a basal diet, whereas the treatment groups were fed basal diets supplemented with L-Carnitine (0 mg/kg, 75 mg/kg and 150 mg/kg) and lysine-methionine (0, 15 and 30%) for 42 days. On day 42, one bird was randomly chosen per replication, a blood sample was taken and the blood concentrations of glucose (GLU), uric acid (UAc), triglyceride (TG), VLDL, HDL, LDL, total protein (TP), albumin (Alb) and total cholesterol (TC) were analyzed. Results. Dietary L-carnitine supplementation had a significant effect (p<0.05) on uric acid (UAc), HDL, LDL, and total cholesterol (TC). The birds feed L-carnitine plus Lys and Met presented the highest plasmatic UAc level and the lowest plasmatic TC and LDL level. Moreover, L-carnitine significantly reduced total cholesterol (TC) when compared with both the control group and the birds feed Lys and Met without L-carnitine. Conclusions. A diet with 150 mg/kg L-carnitine plus 15% Lys and Met seems to be enough to sustain low plasmatic TC, LDL and HDL concentrations on male broiler. RESUMEN Objetivo. Se realizó un estudio para determinar el efecto de la suplementación deL-carnitina, lisina (Lys) y metionina (Met) sobre los metabolitos sanguíneos de pollos de engorde. Materiales y métodos. Se utilizaran 270 pollos de la línea Ross 308 de un día de edad y se dividieron en 9 tratamientos en un diseño al azar con arreglo factorial 3x3: tres niveles de L-carnitina (0 mg/kg, 75 mg/kg y 150 mg/kg) y tres de lisina-metionina (0, 15 y 30%) durante 42 días. Cada tratamiento constó de 3 repeticiones con 10 pollos por repetición. El día 42 de edad, se tomaron muestras de sangre de tres aves por tratamiento para cuantificar niveles séricos de glucosa (GLU), ácido úrico (UAc), triglicéridos (TG), VLDL, HDL, LDL, proteínas totales (PT), albúmina (Alb) y colesterol total (TC). Resultados. La suplementación de L-carnitina en la dieta tuvo un efecto significativo (p<0.05) en los niveles de ácido úrico en suero (UAC), HDL, LDL y colesterol total (CT). Las aves alimentadas con L-carnitina más Lys y Met mostraron niveles séricos más altos de UAc y menor TCy LDL. Por otra parte, la L-carnitina redujó significativamente el colesterol total (CT), cuando se comparó con el grupo control y con los pollos alimentados con Lys y Met, sin L-carnitina. Conclusiones. Una dieta con 150 mg/kg de L-carnitina y 15% Lys y Met parece ser suficiente para mantener bajas concentraciones plasmáticas de TC, LDL y HDL en pollos de engorde.
Author Hosseintabar, Babak
Bouyeh, Mehrdad
Seidavi, Alireza
Dadashbeiki, Mohammad
Gamboa, Sandra
Van den Hoven, René
AuthorAffiliation CERNAS- Centre for Studies on Natural Resources, Environment and Society
Islamic Azad University
Polytechnic Institute
Vetmeduni Wien
AuthorAffiliation_xml – name: Islamic Azad University
– name: Polytechnic Institute
– name: CERNAS- Centre for Studies on Natural Resources, Environment and Society
– name: Vetmeduni Wien
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  givenname: Babak
  surname: Hosseintabar
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  givenname: Mehrdad
  surname: Bouyeh
  fullname: Bouyeh, Mehrdad
– sequence: 4
  givenname: Alireza
  surname: Seidavi
  fullname: Seidavi, Alireza
– sequence: 5
  givenname: René
  surname: Van den Hoven
  fullname: Van den Hoven, René
– sequence: 6
  givenname: Sandra
  surname: Gamboa
  fullname: Gamboa, Sandra
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Issue 3
Keywords serum metabolites
perfil lipídico
amino acids
lipid profile
supplementation
Aminoácidos
metabolitos séricos
suplementación
broiler chicken
pollos
Language English
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Snippet ABSTRACT Objetive. In the present study a completely randomized 3×3 factorial design was used to analyze the effects of different levels of L-Carnitine,...
Objective. In the present study a completely randomized 3x3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys)...
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SubjectTerms AGRICULTURE, DAIRY & ANIMAL SCIENCE
amino acids
Aminoácidos
broiler chicken
lipid profile
metabolitos séricos
perfil lipídico
pollos
serum metabolites
suplementación
supplementation
Title Effect of different levels of L-carnitine and lysine-methionine on broiler blood parameters
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