Isolation and Analysis of Reverse Transcriptase of Ty1-copia-Like Retrotransposons in Sugarcane

• Published in: Sugar tech : an international journal of sugar crops & related industries Vol. 24; no. 5; pp. 1510 - 1529
• Main Authors: Liu, Jun-Xian, Liu, Jing, Yang, Tai-Yi, Liu, Li-Min, Qiu, Li-Hang, Gao, Yi-Jing, Duan, Wei-Xing, Lei, Jing-Chao, Liu, Hong-Jian, Zhang, Rong-Hua, He, Wei-Zhong, Li, Song, Li, Yang-Rui, Wei, Rong-Chang, Xiong, Fa-Qian
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.10.2022; Springer; Springer Nature B.V
• Subjects: Agriculture; Amino acids; Analysis; Biomedical and Life Sciences; Cluster analysis; DNA; DNA polymerases; Genetic transcription; Heterogeneity; Life Sciences; Mutation; Nucleotides; Phylogenetics; Phylogeny; Protein structure; Research Article; retrotransposons; RNA-directed DNA polymerase; Sequences; Similarity; Sugarcane; sugars; Transcription; transcription (genetics); Transposition; Transposons; Heterogeneity; like retrotransposons; Reverse transcriptase; Sugarcane; Ty1

In this study, the reverse transcriptase sequences of Ty1- copia -like retrotransposons were amplified from ROC22 and Badila, and their characteristics, diversity, phylogenetic relationships and transcriptional activity were analyzed. The genomic DNA was amplified by PCR using degenerate primers. Th...