Extracellular ATP induces cell death in human intestinal epithelial cells

• Published in: Biochimica et biophysica acta Vol. 1820; no. 12; pp. 1867 - 1878
• Main Authors: Souza, Carolina O., Santoro, Giani F., Figliuolo, Vanessa R., Nanini, Hayandra Ferreira, de Souza, Heitor S.P., Castelo-Branco, Morgana Teixeira Lima, Abalo, Alessandra Alves, Paiva, Mauricio M., Coutinho, Claudia M.L.M., Coutinho-Silva, Robson
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2012
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Adenosine triphosphate; Adenosine Triphosphate - pharmacology; antioxidants; apoptosis; Apoptosis - drug effects; autophagy; Autophagy - drug effects; Blotting, Western; calcium; Calcium - metabolism; Caspase 3 - metabolism; caspase-3; Cell death; Cells, Cultured; Colon - cytology; Colon - drug effects; Colon - metabolism; epithelial cells; Epithelial Cells - drug effects; Epithelial Cells - metabolism; explants; Flow Cytometry; Fluorescent Antibody Technique; Humans; Ileal Neoplasms - metabolism; Ileal Neoplasms - pathology; immunohistochemistry; interferon-gamma; Intestinal epithelial cell; intestinal mucosa; L-Lactate Dehydrogenase - metabolism; Macrophages - cytology; Macrophages - drug effects; Macrophages - metabolism; Necrosis; nitric oxide; Nitric Oxide - metabolism; Purinergic signaling; Reactive Oxygen Species - metabolism; receptors; secretion; transmission electron microscopy; Cell death; Intestinal epithelial cell; Adenosine triphosphate; Purinergic signaling

Extracellular ATP is an endogenous signaling molecule released by various cell types and under different stimuli. High concentrations of ATP released into the extracellular medium activate the P2X7 receptor in most inflammatory conditions. Here, we seek to characterize the effects of ATP in human in...