Gene profiling in atherosclerosis reveals a key role for small inducible cytokines : Validation using a novel monocyte chemoattractant protein monoclonal antibody

• Published in: Circulation (New York, N.Y.) Vol. 111; no. 25; pp. 3443 - 3452
• Main Authors: LUTGENS, Esther, FABER, Birgit, MACKAY, Charles R, RENNERT, Paul, BAILLY, Veronique, JARPE, Matthew, DOLINSKI, Brian, KOTELIANSKY, Victor, DE FOUGEROLLES, Tony, DAEMEN, Mat J. A. P, SCHAPIRA, Kitty, EVELO, Chris T. A, VAN HAAFTEN, Rachel, HEENEMAN, Sylvia, CLEUTJENS, Kitty B. J. M, BIJNENS, Ann Pascale, BECKERS, Linda, PORTER, J. Gordon
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 28.06.2005
• Subjects: Animals; Antibodies, Monoclonal - administration & dosage; Antibodies, Monoclonal - pharmacology; Aorta, Thoracic; Apolipoproteins E - deficiency; Associated diseases and complications; Atherosclerosis (general aspects, experimental research); Atherosclerosis - drug therapy; Atherosclerosis - genetics; Atherosclerosis - pathology; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Chemokine CCL2 - immunology; Chemokine CCL8; Chemokines - genetics; Chemokines - physiology; Cluster Analysis; Diabetes. Impaired glucose tolerance; Disease Progression; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Extracellular Matrix - metabolism; Gene Expression Profiling; Inflammation - genetics; Male; Medical sciences; Mice; Mice, Knockout; Monocyte Chemoattractant Proteins - immunology; Monocyte Chemoattractant Proteins - physiology; Peptide Hydrolases - genetics; RNA, Messenger - analysis; Time Factors; Vascular disease; Monocyte; genes; Atherosclerosis; Cytokine; Cardiovascular disease; Inflammation; Monoclonal antibody

Pathological aspects of atherosclerosis are well described, but gene profiles during atherosclerotic plaque progression are largely unidentified. Microarray analysis was performed on mRNA of aortic arches of ApoE-/- mice fed normal chow (NC group) or Western-type diet (WD group) for 3, 4.5, and 6 mo...