Neuroprotective activity of natural products isolated from Senecio graciliflorus DC against corticosterone-induced impairment in SH-SY5Y cells

• Published in: Naunyn-Schmiedeberg's archives of pharmacology Vol. 394; no. 12; pp. 2389 - 2399
• Main Authors: Jameel, Salman, Kaur, Loveleena, Bhat, Showkat Ahmad, Malik, Fayaz A., Bhat, Khursheed Ahmad
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2021; Springer Nature B.V
• Subjects: Acetic acid; Acids; AKT protein; Biological activity; Biological Products - isolation & purification; Biological Products - pharmacology; Biomedical and Life Sciences; Biomedicine; Cell death; Cell Death - drug effects; Cell differentiation; Cell Line, Tumor; Cell lines; Cell survival; Cell Survival - drug effects; Corticosterone; Ethyl acetate; Gallic acid; Humans; Metabolic pathways; Natural products; Neuroblastoma; Neuroblastoma - pathology; Neuroblastoma cells; Neurodegeneration; Neurological diseases; Neuroprotection; Neuroprotective Agents - isolation & purification; Neuroprotective Agents - pharmacology; Neurosciences; Original Article; Pharmacology/Toxicology; Plant Extracts - pharmacology; Plant Roots; Secondary Metabolism; Senecio; Senecio - chemistry; Senecio - metabolism; Neuroprotection; Sarcinic acid; Corticosterone; Sterol; Senecio
• DOI: 10.1007/s00210-021-02136-9
• ISSN: 0028-1298

Senecio graciliflorus DC root extract was studied for secondary metabolite composition following the bioactivity-guided isolation technique. The ethyl acetate extract of Senecio graciliflorus root yielded nine chemical constituents: 3,4-di- tert -butyl toluene, stigmasterol, β-sitosterol, 2β-(angeloyloxy)furanoeremophilane, gallic acid, 2β-{[( Z )-2-hydroxymethylbut-2-enoyl]oxy}furanoeremophilane, 1-hydroxypentan-2-yl-4-methylbenzoate, sarcinic acid, and sitosterol 3- O -β- d -glucopyranoside. The structures of the chemical constituents were elucidated on the basis of spectral data analysis in the light of literature. All the compounds are being reported for the first time from this plant. The isolated constituents were screened for neuroprotective effects against corticosterone-induced impairment in neuroblastoma cell lines (SH-SY5S cells). The viability of SH-SY5S cells was determined using MTT assay. Among various isolated compounds, three natural products (sarcinic acid, gallic acid, and β-sitosterol) displayed robust neurotropic activity. The compounds increased neuronal cell survival in differentiated neuroblastoma cells (SH-SY5Y) from high-dose corticosterone (400 µM)–induced cell death. All the three constituents showed maximum AKT/ERK pathway activation at 20 µM concentration. The studies are aimed to explore small molecules for treating neurodegeneration underlying various neurological disorders to restore neuronal cell plasticity.