Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code

MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes...

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Published inBiosensors (Basel) Vol. 13; no. 12; p. 1033
Main Authors Tomagra, Giulia, Re, Alice, Varzi, Veronica, Aprà, Pietro, Britel, Adam, Franchino, Claudio, Sturari, Sofia, Amine, Nour-Hanne, Westerink, Remco H S, Carabelli, Valentina, Picollo, Federico
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.12.2023
Subjects
Amperometry
Arrays
Automation
Biocompatibility
Biosensors
Carbon
Computer programming
Data acquisition
Design and construction
diamond
Diamond machining
dopamine
Electrical measurement
Electrochemical apparatus
Electrodes
Engineering research
Exocytosis
Graphite
Ion beams
Levodopa
Membrane fusion
Methods
Microelectrodes
Micrography
Microscopy
multi-electrode array
Neurosecretory cells
Oxidation
Parameters
PC12 cell line
Pheochromocytoma cells
Point defects
Signal detection
Italy
amperometry
diamond
exocytosis
PC12 cell line
dopamine
multi-electrode array
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Abstract MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes, each of them recording large amounts of data revealing the exocytotic activity, the aim of this work was to support an adequate analysis code to speed up the signal detection. The cutting-edge technology of microGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) has been implemented with an automated analysis code (APE, Amperometric Peak Analysis) developed using Matlab R2022a software to provide easy and accurate detection of amperometric spike parameters, including the analysis of the pre-spike foot that sometimes precedes the complete fusion pore dilatation. Data have been acquired from cultured PC12 cells, either collecting events during spontaneous exocytosis or after L-DOPA incubation. Validation of the APE code was performed by comparing the acquired spike parameters with those obtained using Quanta Analysis (Igor macro) by Mosharov et al.
AbstractList MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes, each of them recording large amounts of data revealing the exocytotic activity, the aim of this work was to support an adequate analysis code to speed up the signal detection. The cutting-edge technology of microGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) has been implemented with an automated analysis code (APE, Amperometric Peak Analysis) developed using Matlab R2022a software to provide easy and accurate detection of amperometric spike parameters, including the analysis of the pre-spike foot that sometimes precedes the complete fusion pore dilatation. Data have been acquired from cultured PC12 cells, either collecting events during spontaneous exocytosis or after L-DOPA incubation. Validation of the APE code was performed by comparing the acquired spike parameters with those obtained using Quanta Analysis (Igor macro) by Mosharov et al.
Audience Academic
Author Carabelli, Valentina
Varzi, Veronica
Britel, Adam
Aprà, Pietro
Westerink, Remco H S
Tomagra, Giulia
Amine, Nour-Hanne
Franchino, Claudio
Re, Alice
Sturari, Sofia
Picollo, Federico
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– sequence: 8
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  surname: Amine
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  orcidid: 0000-0003-4948-2007
  surname: Westerink
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– sequence: 10
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  givenname: Federico
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  surname: Picollo
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  organization: Department of Physics, NIS Interdepartmental Centre, University of Torino and Italian Institute of Nuclear Physics, Via Giuria 1, 10125 Torino, Italy
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dopamine
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Snippet MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many...
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Aggregation Database
Index Database
StartPage 1033
SubjectTerms Amperometry
Arrays
Automation
Biocompatibility
Biosensors
Carbon
Computer programming
Data acquisition
Design and construction
diamond
Diamond machining
dopamine
Electrical measurement
Electrochemical apparatus
Electrodes
Engineering research
Exocytosis
Graphite
Ion beams
Levodopa
Membrane fusion
Methods
Microelectrodes
Micrography
Microscopy
multi-electrode array
Neurosecretory cells
Oxidation
Parameters
PC12 cell line
Pheochromocytoma cells
Point defects
Signal detection
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Title Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code
URI https://www.ncbi.nlm.nih.gov/pubmed/38131793
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