Comparison of the thin-layer chromatographic properties of sulfur-containing amino acids and their aminophosphonic analogues

• Published in: Journal of Chromatography A Vol. 888; no. 1; pp. 335 - 339
• Main Authors: Kudzin, Zbigniew H., Saganiak, Monika, Kotyński, Andrzej, Ciesielski, Witold
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 04.08.2000; Elsevier
• Subjects: Amino acids; Amino Acids - chemistry; Aminophosphonic acids; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Thin Layer - methods; Exact sciences and technology; Organophosphonates - chemistry; Other chromatographic methods; Sulfur - chemistry; Sulfur compounds; Sulfur compounds; Amino acids; Aminophosphonic acids; Cysteine; Organic azide; Ninhydrin; Chromatographic retention; Methionine; Phosphonic acids; Experimental study; Molybdates; Phosphorus Organic compounds; Sulfur containing aminoacid; Thin layer chromatography; Aminoacid; Detection limit; Detection; Iodine
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)00498-2

The thin-layer chromatographic behavior of the natural, sulfur-containing amino acids (Cys, Hcys, Met) and their phosphonic analogues (Cys P, Hcys P, Met P) has been studied. Their detection limits using iodine and molybdate, ninhydrine and iodine–azide reagents were determined, and the R F values of these two classes of amino acids in acidic, neutral and mild basic solvent systems were compared.