Efficacy of thiolated eudragit microspheres as an oral vaccine delivery system to induce mucosal immunity against enterotoxigenic Escherichia coli in mice
To investigate the efficay of thiolated eudragit microspheres (TEMS) as an oral vaccine delivery system, F4 and F18 in E.coli were loaded in TEMS and orally immunized to mice. Induced immune responses such as IgG an IgA levels were investigated with sera, saliva, feces, splenocyte, peyer’s patch, la...
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| Published in | European journal of pharmaceutics and biopharmaceutics Vol. 81; no. 1; pp. 43 - 48 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Amsterdam
Elsevier B.V
01.05.2012
Elsevier |
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| Abstract | To investigate the efficay of thiolated eudragit microspheres (TEMS) as an oral vaccine delivery system, F4 and F18 in E.coli were loaded in TEMS and orally immunized to mice. Induced immune responses such as IgG an IgA levels were investigated with sera, saliva, feces, splenocyte, peyer’s patch, lamina propria cells in mice.
A vaccine delivery system based on thiolated eudragit microsphere (TEMS) was studied in vivo for its ability to elicit mucosal immunity against enterotoxigenic Escherichia coli (ETEC). Groups of mice were orally immunized with F4 or F18 fimbriae of ETEC and F4 or F18 loaded in TEMS. Mice that were orally administered with F4 or F18 loaded TEMS showed higher antigen-specific IgG antibody responses in serum and antigen-specific IgA in saliva and feces than mice that were immunized with antigens only. In addition, oral vaccination of F4 or F18 loaded TEMS resulted in higher numbers of IgG and IgA antigen-specific antibody secreting cells in the spleen, lamina propria, and Peyer’s patches of immunized mice than other groups. Moreover, TEMS administration loaded with F4 or F18 induced mixed Th1 and Th2 type responses based on similarly increased levels of IgG1 and IgG2a. These results suggest that F4 or F18 loaded TEMS may be a promising candidate for an oral vaccine delivery system to elicit systemic and mucosal immunity against ETEC. |
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| AbstractList | A vaccine delivery system based on thiolated eudragit microsphere (TEMS) was studied in vivo for its ability to elicit mucosal immunity against enterotoxigenic Escherichia coli (ETEC). Groups of mice were orally immunized with F4 or F18 fimbriae of ETEC and F4 or F18 loaded in TEMS. Mice that were orally administered with F4 or F18 loaded TEMS showed higher antigen-specific IgG antibody responses in serum and antigen-specific IgA in saliva and feces than mice that were immunized with antigens only. In addition, oral vaccination of F4 or F18 loaded TEMS resulted in higher numbers of IgG and IgA antigen-specific antibody secreting cells in the spleen, lamina propria, and Peyer's patches of immunized mice than other groups. Moreover, TEMS administration loaded with F4 or F18 induced mixed Th1 and Th2 type responses based on similarly increased levels of IgG1 and IgG2a. These results suggest that F4 or F18 loaded TEMS may be a promising candidate for an oral vaccine delivery system to elicit systemic and mucosal immunity against ETEC. To investigate the efficay of thiolated eudragit microspheres (TEMS) as an oral vaccine delivery system, F4 and F18 in E.coli were loaded in TEMS and orally immunized to mice. Induced immune responses such as IgG an IgA levels were investigated with sera, saliva, feces, splenocyte, peyer’s patch, lamina propria cells in mice. A vaccine delivery system based on thiolated eudragit microsphere (TEMS) was studied in vivo for its ability to elicit mucosal immunity against enterotoxigenic Escherichia coli (ETEC). Groups of mice were orally immunized with F4 or F18 fimbriae of ETEC and F4 or F18 loaded in TEMS. Mice that were orally administered with F4 or F18 loaded TEMS showed higher antigen-specific IgG antibody responses in serum and antigen-specific IgA in saliva and feces than mice that were immunized with antigens only. In addition, oral vaccination of F4 or F18 loaded TEMS resulted in higher numbers of IgG and IgA antigen-specific antibody secreting cells in the spleen, lamina propria, and Peyer’s patches of immunized mice than other groups. Moreover, TEMS administration loaded with F4 or F18 induced mixed Th1 and Th2 type responses based on similarly increased levels of IgG1 and IgG2a. These results suggest that F4 or F18 loaded TEMS may be a promising candidate for an oral vaccine delivery system to elicit systemic and mucosal immunity against ETEC. A vaccine delivery system based on thiolated eudragit microsphere (TEMS) was studied in vivo for its ability to elicit mucosal immunity against enterotoxigenic Escherichia coli (ETEC). Groups of mice were orally immunized with F4 or F18 fimbriae of ETEC and F4 or F18 loaded in TEMS. Mice that were orally administered with F4 or F18 loaded TEMS showed higher antigen-specific IgG antibody responses in serum and antigen-specific IgA in saliva and feces than mice that were immunized with antigens only. In addition, oral vaccination of F4 or F18 loaded TEMS resulted in higher numbers of IgG and IgA antigen-specific antibody secreting cells in the spleen, lamina propria, and Peyeras patches of immunized mice than other groups. Moreover, TEMS administration loaded with F4 or F18 induced mixed Th1 and Th2 type responses based on similarly increased levels of IgG1 and IgG2a. These results suggest that F4 or F18 loaded TEMS may be a promising candidate for an oral vaccine delivery system to elicit systemic and mucosal immunity against ETEC. |
| Author | Cho, Chong-su Yoo, Han Sang Islam, Mohammad Ariful Lee, Won-Jung Cha, Seungbin Jung, Myunghwan Shin, Minkyoung |
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| CitedBy_id | crossref_primary_10_1016_j_addr_2021_113928 crossref_primary_10_1016_j_jconrel_2016_05_058 crossref_primary_10_1016_j_ejps_2015_09_014 crossref_primary_10_1016_j_colsurfb_2013_02_036 crossref_primary_10_1016_j_ijpharm_2018_03_009 crossref_primary_10_1016_j_addr_2021_113867 crossref_primary_10_1038_s41598_020_77551_8 crossref_primary_10_3390_ma7031555 crossref_primary_10_3390_agriculture11030186 crossref_primary_10_1016_j_micpath_2018_02_032 crossref_primary_10_1016_j_biomaterials_2018_10_041 crossref_primary_10_1007_s12602_021_09773_9 crossref_primary_10_1016_j_jddst_2024_105889 crossref_primary_10_7569_RAA_2013_097307 crossref_primary_10_1016_j_compositesb_2022_109826 crossref_primary_10_2174_1567201817666200512093639 |
| Cites_doi | 10.7883/yoken.63.49 10.1023/A:1018830204170 10.1016/S0168-3659(02)00411-X 10.1248/bpb.26.969 10.1016/j.coi.2009.04.002 10.1016/S0378-1135(99)00076-0 10.1016/S0378-1135(96)01293-X 10.1128/IAI.67.2.520-526.1999 10.1016/j.vetimm.2007.06.018 10.1007/s11259-010-9418-x 10.1292/jvms.59.917 10.1016/S0022-1759(98)00170-7 10.1038/nri2735 10.1016/j.rvsc.2010.06.003 10.1128/IAI.61.10.4272-4279.1993 10.1016/j.ijpharm.2008.04.003 10.1080/10611860600648346 10.1128/CMR.11.1.142 10.1042/bj1760429 10.1177/104063870802000413 10.1016/S0378-5173(01)00807-9 10.1016/S0264-410X(02)00220-7 10.1016/S0264-410X(98)00406-X 10.1016/S0928-8244(97)00035-7 10.2174/156652410791065417 10.4049/jimmunol.164.6.3047 10.1271/bbb1961.49.1335 10.1016/S0264-410X(00)00094-3 10.1016/S0939-6411(03)00061-4 10.1016/j.ijpharm.2006.06.019 10.1016/j.ijpharm.2006.11.014 10.1016/j.ejpb.2011.04.016 10.1021/bi00377a005 10.1007/978-3-642-74529-4_6 10.1016/j.colsurfb.2006.09.012 |
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| Keywords | Delivery system Mice Microsphere Immune response In vivo test Pharmaceutical technology Escherichia coli Thiolate Rodentia Oral administration Vaccine Immunity In vivo Vertebrata Mammalia Mouse Efficiency Animal Bacteria Microparticle Enterobacteriaceae |
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| References | Takagi, Teshima, Okunuki, Sawada (b0020) 2003; 26 Liu, Li, Xu, Wang (b0155) 2010; 34 MacDonald, Carter (b0135) 1982; 45 Walker, Taylor (b0010) 1978; 176 Fushiki, Yamamoto, Iwai (b0025) 1985; 49 Kunisawa, Okudaira, Tsutusmi, Takahashi, Nakanishi, Kiyono, Mayumi (b0015) 2000; 19 Eldridge, Gilley, Staas, Moldoveanu, Meulbroek, Tice (b0175) 1989; 146 Imberechts, Deprez, Van Driessche, Pohl (b0080) 1997; 54 Lee, Cha, Shin, Islam, Cho, Yoo (b0115) 2011; 79 Meissner, Ubrich, El Ghazouani, Maincent, Lamprecht (b0045) 2007; 335 Verdonck, Cox, van Gog, Van der Stede, Duchateau, Deprez, Goddeeris (b0110) 2002; 20 Takeuchi, Matsui, Yamamoto, Kawashima (b0040) 2003; 86 Quan, Jiang, Kim, Jeong, Choi, Guo, Yoo, Lee, Cho (b0065) 2008; 359 Jackson, Fujihashi, Xu-Amano, Kiyono, Elson, McGhee (b0140) 1993; 61 Jankovic, Kullberg, Noben-Trauth, Caspar, Paul, Sher (b0180) 2000; 164 Zuniga, Yokoyama, Albicker-Rippinger, Eggenberger, Bertschinger (b0095) 1997; 18 Nataro, Kaper (b0070) 1998; 11 Gyles, International (b0075) 1994 Frey, Di Canzio, Zurakowski (b0125) 1998; 221 Yokoyama, Hashi, Umeda, Icatlo, Kuroki, Ikemori, Kodama (b0090) 1997; 59 Cha, Rayamajhi, Kang, Lee, Shin, Yoo (b0130) 2010; 63 Van den Broeck, Cox, Goddeeris (b0145) 1999; 17 Bernkop-Schnurch, Schwarz, Steininger (b0165) 1999; 16 Leitner, Walker, Bernkop-Schnurch (b0055) 2003; 56 Sung, Kang, Lee, Shin, Lim, Kim, Song, Yoo (b0120) 2011; 90 Verdonck, Tiels, van Gog, Goddeeris, Lycke, Clements, Cox (b0150) 2007; 120 Snyder (b0160) 1987; 26 Thongborisute, Tsuruta, Kawabata, Takeuchi (b0035) 2006; 14 Lee, Kang, Kang, Yoo (b0100) 2008; 20 Paul, Zhu (b0185) 2010; 10 Takahashi, Nochi, Yuki, Kiyono (b0005) 2009; 21 Van den Broeck, Cox, Goddeeris (b0085) 1999; 67 Sajeesh, Sharma (b0030) 2006; 325 Sarmento, Ribeiro, Veiga, Ferreira (b0050) 2006; 53 Van den Broeck, Cox, Goddeeris (b0105) 1999; 68 Malik, Goyal, Mangal, Zakir, Vyas (b0170) 2010; 10 Bernkop-Schnurch, Clausen, Hnatyszyn (b0060) 2001; 226 Liu (10.1016/j.ejpb.2012.01.010_b0155) 2010; 34 Takagi (10.1016/j.ejpb.2012.01.010_b0020) 2003; 26 Bernkop-Schnurch (10.1016/j.ejpb.2012.01.010_b0060) 2001; 226 Takeuchi (10.1016/j.ejpb.2012.01.010_b0040) 2003; 86 Verdonck (10.1016/j.ejpb.2012.01.010_b0150) 2007; 120 MacDonald (10.1016/j.ejpb.2012.01.010_b0135) 1982; 45 Lee (10.1016/j.ejpb.2012.01.010_b0100) 2008; 20 Yokoyama (10.1016/j.ejpb.2012.01.010_b0090) 1997; 59 Quan (10.1016/j.ejpb.2012.01.010_b0065) 2008; 359 Jackson (10.1016/j.ejpb.2012.01.010_b0140) 1993; 61 Fushiki (10.1016/j.ejpb.2012.01.010_b0025) 1985; 49 Sarmento (10.1016/j.ejpb.2012.01.010_b0050) 2006; 53 Verdonck (10.1016/j.ejpb.2012.01.010_b0110) 2002; 20 Walker (10.1016/j.ejpb.2012.01.010_b0010) 1978; 176 Takahashi (10.1016/j.ejpb.2012.01.010_b0005) 2009; 21 Eldridge (10.1016/j.ejpb.2012.01.010_b0175) 1989; 146 Nataro (10.1016/j.ejpb.2012.01.010_b0070) 1998; 11 Sung (10.1016/j.ejpb.2012.01.010_b0120) 2011; 90 Frey (10.1016/j.ejpb.2012.01.010_b0125) 1998; 221 Leitner (10.1016/j.ejpb.2012.01.010_b0055) 2003; 56 Paul (10.1016/j.ejpb.2012.01.010_b0185) 2010; 10 Malik (10.1016/j.ejpb.2012.01.010_b0170) 2010; 10 Cha (10.1016/j.ejpb.2012.01.010_b0130) 2010; 63 Van den Broeck (10.1016/j.ejpb.2012.01.010_b0085) 1999; 67 Van den Broeck (10.1016/j.ejpb.2012.01.010_b0105) 1999; 68 Jankovic (10.1016/j.ejpb.2012.01.010_b0180) 2000; 164 Snyder (10.1016/j.ejpb.2012.01.010_b0160) 1987; 26 Bernkop-Schnurch (10.1016/j.ejpb.2012.01.010_b0165) 1999; 16 Lee (10.1016/j.ejpb.2012.01.010_b0115) 2011; 79 Kunisawa (10.1016/j.ejpb.2012.01.010_b0015) 2000; 19 Thongborisute (10.1016/j.ejpb.2012.01.010_b0035) 2006; 14 Gyles (10.1016/j.ejpb.2012.01.010_b0075) 1994 Sajeesh (10.1016/j.ejpb.2012.01.010_b0030) 2006; 325 Meissner (10.1016/j.ejpb.2012.01.010_b0045) 2007; 335 Zuniga (10.1016/j.ejpb.2012.01.010_b0095) 1997; 18 Van den Broeck (10.1016/j.ejpb.2012.01.010_b0145) 1999; 17 Imberechts (10.1016/j.ejpb.2012.01.010_b0080) 1997; 54 |
| References_xml | – year: 1994 ident: b0075 article-title: in Domestic Animals and Humans contributor: fullname: International – volume: 68 start-page: 255 year: 1999 end-page: 263 ident: b0105 article-title: Receptor-specific binding of purified F4 to isolated villi publication-title: Vet. Microbiol. contributor: fullname: Goddeeris – volume: 45 start-page: 769 year: 1982 end-page: 774 ident: b0135 article-title: Isolation and functional characteristics of adherent phagocytic cells from mouse Peyer’s patches publication-title: Immunology contributor: fullname: Carter – volume: 90 start-page: 329 year: 2011 end-page: 335 ident: b0120 article-title: Improved sero-monitoring assay for classical swine fever (CSF) using the recombinant E2 protein of a recent Korean isolate publication-title: Res. Vet. Sci. contributor: fullname: Yoo – volume: 10 start-page: 225 year: 2010 end-page: 235 ident: b0185 article-title: How are T(H)2-type immune responses initiated and amplified? publication-title: Nat. Rev. Immunol. contributor: fullname: Zhu – volume: 49 start-page: 1335 year: 1985 end-page: 1342 ident: b0025 article-title: Investigation of digestion and absorption of a dietary-protein by using its antigenicity as an index – gastrointestinal digestion of ovalbumin publication-title: Agr. Biol. Chem. contributor: fullname: Iwai – volume: 56 start-page: 207 year: 2003 end-page: 214 ident: b0055 article-title: Thiolated polymers: evidence for the formation of disulphide bonds with mucus glycoproteins publication-title: Eur. J. Pharm. Biopharm. contributor: fullname: Bernkop-Schnurch – volume: 17 start-page: 2020 year: 1999 end-page: 2029 ident: b0145 article-title: Induction of immune responses in pigs following oral administration of purified F4 fimbriae publication-title: Vaccine contributor: fullname: Goddeeris – volume: 67 start-page: 520 year: 1999 end-page: 526 ident: b0085 article-title: Receptor-dependent immune responses in pigs after oral immunization with F4 fimbriae publication-title: Infect. Immun. contributor: fullname: Goddeeris – volume: 18 start-page: 153 year: 1997 end-page: 161 ident: b0095 article-title: Reduced intestinal colonisation with F18-positive enterotoxigenic publication-title: FEMS Immunol. Med. Microbiol. contributor: fullname: Bertschinger – volume: 34 start-page: 491 year: 2010 end-page: 502 ident: b0155 article-title: Immune responses elicited in mice with recombinant publication-title: Vet. Res. Commun. contributor: fullname: Wang – volume: 20 start-page: 492 year: 2008 end-page: 496 ident: b0100 article-title: Development of multiplex polymerase chain reaction assays for detecting enterotoxigenic publication-title: J. Vet. Diagn. Invest. contributor: fullname: Yoo – volume: 221 start-page: 35 year: 1998 end-page: 41 ident: b0125 article-title: A statistically defined endpoint titer determination method for immunoassays publication-title: J. Immunol. Methods contributor: fullname: Zurakowski – volume: 61 start-page: 4272 year: 1993 end-page: 4279 ident: b0140 article-title: Optimizing oral vaccines: induction of systemic and mucosal B-cell and antibody responses to tetanus toxoid by use of cholera toxin as an adjuvant publication-title: Infect. Immun. contributor: fullname: McGhee – volume: 146 start-page: 59 year: 1989 end-page: 66 ident: b0175 article-title: Biodegradable microspheres: vaccine delivery system for oral immunization publication-title: Curr. Top Microbiol. Immunol. contributor: fullname: Tice – volume: 16 start-page: 876 year: 1999 end-page: 881 ident: b0165 article-title: Polymers with thiol groups: a new generation of mucoadhesive polymers? publication-title: Pharm. Res. contributor: fullname: Steininger – volume: 53 start-page: 193 year: 2006 end-page: 202 ident: b0050 article-title: Development and characterization of new insulin containing polysaccharide nanoparticles publication-title: Colloids Surf. B. Biointerfaces contributor: fullname: Ferreira – volume: 59 start-page: 917 year: 1997 end-page: 921 ident: b0090 article-title: Effect of oral egg antibody in experimental F18(+) publication-title: J. Vet. Med. Sci. contributor: fullname: Kodama – volume: 63 start-page: 49 year: 2010 end-page: 51 ident: b0130 article-title: Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of publication-title: Jpn. J. Infect. Dis. contributor: fullname: Yoo – volume: 11 start-page: 142 year: 1998 end-page: 201 ident: b0070 article-title: Diarrheagenic publication-title: Clin. Microbiol. Rev. contributor: fullname: Kaper – volume: 120 start-page: 69 year: 2007 end-page: 79 ident: b0150 article-title: Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ publication-title: Vet. Immunol. Immunopathol. contributor: fullname: Cox – volume: 359 start-page: 205 year: 2008 end-page: 210 ident: b0065 article-title: PH-sensitive and mucoadhesive thiolated eudragit-coated chitosan microspheres publication-title: Int. J. Pharm. contributor: fullname: Cho – volume: 20 start-page: 2995 year: 2002 end-page: 3004 ident: b0110 article-title: Different kinetic of antibody responses following infection of newly weaned pigs with an F4 enterotoxigenic publication-title: Vaccine contributor: fullname: Goddeeris – volume: 226 start-page: 185 year: 2001 end-page: 194 ident: b0060 article-title: Thiolated polymers: synthesis and in vitro evaluation of polymer-cysteamine conjugates publication-title: Int. J. Pharm. contributor: fullname: Hnatyszyn – volume: 14 start-page: 147 year: 2006 end-page: 154 ident: b0035 article-title: The effect of particle structure of chitosan-coated liposomes and type of chitosan on oral delivery of calcitonin publication-title: J. Drug. Target. contributor: fullname: Takeuchi – volume: 26 start-page: 688 year: 1987 end-page: 694 ident: b0160 article-title: Intramolecular disulfide loop formation in a peptide containing two cysteines publication-title: Biochemistry contributor: fullname: Snyder – volume: 26 start-page: 969 year: 2003 end-page: 973 ident: b0020 article-title: Comparative study of in vitro digestibility of food proteins and effect of preheating on the digestion publication-title: Biol. Pharm. Bull. contributor: fullname: Sawada – volume: 21 start-page: 352 year: 2009 end-page: 358 ident: b0005 article-title: New horizon of mucosal immunity and vaccines publication-title: Curr. Opin. Immunol. contributor: fullname: Kiyono – volume: 325 start-page: 147 year: 2006 end-page: 154 ident: b0030 article-title: Cyclodextrin–insulin complex encapsulated polymethacrylic acid based nanoparticles for oral insulin delivery publication-title: Int. J. Pharm. contributor: fullname: Sharma – volume: 54 start-page: 329 year: 1997 end-page: 341 ident: b0080 article-title: Chicken egg yolk antibodies against F18ab fimbriae of publication-title: Vet. Microbiol. contributor: fullname: Pohl – volume: 176 start-page: 429 year: 1978 end-page: 432 ident: b0010 article-title: Ovalbumin digestion by human pepsins 1, 3 and 5 publication-title: Biochem. J. contributor: fullname: Taylor – volume: 19 start-page: 589 year: 2000 end-page: 594 ident: b0015 article-title: Characterization of mucoadhesive microspheres for the induction of mucosal and systemic immune responses publication-title: Vaccine contributor: fullname: Mayumi – volume: 10 start-page: 47 year: 2010 end-page: 70 ident: b0170 article-title: Implication of gut immunology in the design of oral vaccines publication-title: Curr. Mol. Med. contributor: fullname: Vyas – volume: 164 start-page: 3047 year: 2000 end-page: 3055 ident: b0180 article-title: Single cell analysis reveals that IL-4 receptor/Stat6 signaling is not required for the in vivo or in vitro development of CD4+ lymphocytes with a Th2 cytokine profile publication-title: J. Immunol. contributor: fullname: Sher – volume: 335 start-page: 147 year: 2007 end-page: 153 ident: b0045 article-title: Low molecular weight heparin loaded pH-sensitive microparticles publication-title: Int. J. Pharm. contributor: fullname: Lamprecht – volume: 79 start-page: 226 year: 2011 end-page: 231 ident: b0115 article-title: Induction of Th1 polarized immune responses by thiolated eudragit-coated F4 and F18 fimbriae of enterotoxigenic publication-title: Eur. J. Pharm. Biopharm. contributor: fullname: Yoo – volume: 86 start-page: 235 year: 2003 end-page: 242 ident: b0040 article-title: Mucoadhesive properties of carbopol or chitosan-coated liposomes and their effectiveness in the oral administration of calcitonin to rats publication-title: J. Control. Release contributor: fullname: Kawashima – volume: 63 start-page: 49 year: 2010 ident: 10.1016/j.ejpb.2012.01.010_b0130 article-title: Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus publication-title: Jpn. J. Infect. Dis. doi: 10.7883/yoken.63.49 contributor: fullname: Cha – volume: 16 start-page: 876 year: 1999 ident: 10.1016/j.ejpb.2012.01.010_b0165 article-title: Polymers with thiol groups: a new generation of mucoadhesive polymers? publication-title: Pharm. Res. doi: 10.1023/A:1018830204170 contributor: fullname: Bernkop-Schnurch – volume: 86 start-page: 235 year: 2003 ident: 10.1016/j.ejpb.2012.01.010_b0040 article-title: Mucoadhesive properties of carbopol or chitosan-coated liposomes and their effectiveness in the oral administration of calcitonin to rats publication-title: J. Control. Release doi: 10.1016/S0168-3659(02)00411-X contributor: fullname: Takeuchi – year: 1994 ident: 10.1016/j.ejpb.2012.01.010_b0075 contributor: fullname: Gyles – volume: 26 start-page: 969 year: 2003 ident: 10.1016/j.ejpb.2012.01.010_b0020 article-title: Comparative study of in vitro digestibility of food proteins and effect of preheating on the digestion publication-title: Biol. Pharm. Bull. doi: 10.1248/bpb.26.969 contributor: fullname: Takagi – volume: 21 start-page: 352 year: 2009 ident: 10.1016/j.ejpb.2012.01.010_b0005 article-title: New horizon of mucosal immunity and vaccines publication-title: Curr. Opin. Immunol. doi: 10.1016/j.coi.2009.04.002 contributor: fullname: Takahashi – volume: 68 start-page: 255 year: 1999 ident: 10.1016/j.ejpb.2012.01.010_b0105 article-title: Receptor-specific binding of purified F4 to isolated villi publication-title: Vet. Microbiol. doi: 10.1016/S0378-1135(99)00076-0 contributor: fullname: Van den Broeck – volume: 54 start-page: 329 year: 1997 ident: 10.1016/j.ejpb.2012.01.010_b0080 article-title: Chicken egg yolk antibodies against F18ab fimbriae of Escherichia coli inhibit shedding of F18 positive E. coli by experimentally infected pigs publication-title: Vet. Microbiol. doi: 10.1016/S0378-1135(96)01293-X contributor: fullname: Imberechts – volume: 67 start-page: 520 year: 1999 ident: 10.1016/j.ejpb.2012.01.010_b0085 article-title: Receptor-dependent immune responses in pigs after oral immunization with F4 fimbriae publication-title: Infect. Immun. doi: 10.1128/IAI.67.2.520-526.1999 contributor: fullname: Van den Broeck – volume: 120 start-page: 69 year: 2007 ident: 10.1016/j.ejpb.2012.01.010_b0150 article-title: Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection publication-title: Vet. Immunol. Immunopathol. doi: 10.1016/j.vetimm.2007.06.018 contributor: fullname: Verdonck – volume: 34 start-page: 491 year: 2010 ident: 10.1016/j.ejpb.2012.01.010_b0155 article-title: Immune responses elicited in mice with recombinant Lactococcus lactis expressing F4 fimbrial adhesin FaeG by oral immunization publication-title: Vet. Res. Commun. doi: 10.1007/s11259-010-9418-x contributor: fullname: Liu – volume: 59 start-page: 917 year: 1997 ident: 10.1016/j.ejpb.2012.01.010_b0090 article-title: Effect of oral egg antibody in experimental F18(+) Escherichia coli infection in weaned pigs publication-title: J. Vet. Med. Sci. doi: 10.1292/jvms.59.917 contributor: fullname: Yokoyama – volume: 221 start-page: 35 year: 1998 ident: 10.1016/j.ejpb.2012.01.010_b0125 article-title: A statistically defined endpoint titer determination method for immunoassays publication-title: J. Immunol. Methods doi: 10.1016/S0022-1759(98)00170-7 contributor: fullname: Frey – volume: 10 start-page: 225 year: 2010 ident: 10.1016/j.ejpb.2012.01.010_b0185 article-title: How are T(H)2-type immune responses initiated and amplified? publication-title: Nat. Rev. Immunol. doi: 10.1038/nri2735 contributor: fullname: Paul – volume: 90 start-page: 329 year: 2011 ident: 10.1016/j.ejpb.2012.01.010_b0120 article-title: Improved sero-monitoring assay for classical swine fever (CSF) using the recombinant E2 protein of a recent Korean isolate publication-title: Res. Vet. Sci. doi: 10.1016/j.rvsc.2010.06.003 contributor: fullname: Sung – volume: 61 start-page: 4272 year: 1993 ident: 10.1016/j.ejpb.2012.01.010_b0140 article-title: Optimizing oral vaccines: induction of systemic and mucosal B-cell and antibody responses to tetanus toxoid by use of cholera toxin as an adjuvant publication-title: Infect. Immun. doi: 10.1128/IAI.61.10.4272-4279.1993 contributor: fullname: Jackson – volume: 359 start-page: 205 year: 2008 ident: 10.1016/j.ejpb.2012.01.010_b0065 article-title: PH-sensitive and mucoadhesive thiolated eudragit-coated chitosan microspheres publication-title: Int. J. Pharm. doi: 10.1016/j.ijpharm.2008.04.003 contributor: fullname: Quan – volume: 45 start-page: 769 year: 1982 ident: 10.1016/j.ejpb.2012.01.010_b0135 article-title: Isolation and functional characteristics of adherent phagocytic cells from mouse Peyer’s patches publication-title: Immunology contributor: fullname: MacDonald – volume: 14 start-page: 147 year: 2006 ident: 10.1016/j.ejpb.2012.01.010_b0035 article-title: The effect of particle structure of chitosan-coated liposomes and type of chitosan on oral delivery of calcitonin publication-title: J. Drug. Target. doi: 10.1080/10611860600648346 contributor: fullname: Thongborisute – volume: 11 start-page: 142 year: 1998 ident: 10.1016/j.ejpb.2012.01.010_b0070 article-title: Diarrheagenic Escherichia coli publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.11.1.142 contributor: fullname: Nataro – volume: 176 start-page: 429 year: 1978 ident: 10.1016/j.ejpb.2012.01.010_b0010 article-title: Ovalbumin digestion by human pepsins 1, 3 and 5 publication-title: Biochem. J. doi: 10.1042/bj1760429 contributor: fullname: Walker – volume: 20 start-page: 492 year: 2008 ident: 10.1016/j.ejpb.2012.01.010_b0100 article-title: Development of multiplex polymerase chain reaction assays for detecting enterotoxigenic Escherichia coli and their application to field isolates from piglets with diarrhea publication-title: J. Vet. Diagn. Invest. doi: 10.1177/104063870802000413 contributor: fullname: Lee – volume: 226 start-page: 185 year: 2001 ident: 10.1016/j.ejpb.2012.01.010_b0060 article-title: Thiolated polymers: synthesis and in vitro evaluation of polymer-cysteamine conjugates publication-title: Int. J. Pharm. doi: 10.1016/S0378-5173(01)00807-9 contributor: fullname: Bernkop-Schnurch – volume: 20 start-page: 2995 year: 2002 ident: 10.1016/j.ejpb.2012.01.010_b0110 article-title: Different kinetic of antibody responses following infection of newly weaned pigs with an F4 enterotoxigenic Escherichia coli strain or an F18 verotoxigenic Escherichia coli strain publication-title: Vaccine doi: 10.1016/S0264-410X(02)00220-7 contributor: fullname: Verdonck – volume: 17 start-page: 2020 year: 1999 ident: 10.1016/j.ejpb.2012.01.010_b0145 article-title: Induction of immune responses in pigs following oral administration of purified F4 fimbriae publication-title: Vaccine doi: 10.1016/S0264-410X(98)00406-X contributor: fullname: Van den Broeck – volume: 18 start-page: 153 year: 1997 ident: 10.1016/j.ejpb.2012.01.010_b0095 article-title: Reduced intestinal colonisation with F18-positive enterotoxigenic Escherichia coli in weaned pigs fed chicken egg antibody against the fimbriae publication-title: FEMS Immunol. Med. Microbiol. doi: 10.1016/S0928-8244(97)00035-7 contributor: fullname: Zuniga – volume: 10 start-page: 47 year: 2010 ident: 10.1016/j.ejpb.2012.01.010_b0170 article-title: Implication of gut immunology in the design of oral vaccines publication-title: Curr. Mol. Med. doi: 10.2174/156652410791065417 contributor: fullname: Malik – volume: 164 start-page: 3047 year: 2000 ident: 10.1016/j.ejpb.2012.01.010_b0180 article-title: Single cell analysis reveals that IL-4 receptor/Stat6 signaling is not required for the in vivo or in vitro development of CD4+ lymphocytes with a Th2 cytokine profile publication-title: J. Immunol. doi: 10.4049/jimmunol.164.6.3047 contributor: fullname: Jankovic – volume: 49 start-page: 1335 year: 1985 ident: 10.1016/j.ejpb.2012.01.010_b0025 article-title: Investigation of digestion and absorption of a dietary-protein by using its antigenicity as an index – gastrointestinal digestion of ovalbumin publication-title: Agr. Biol. Chem. doi: 10.1271/bbb1961.49.1335 contributor: fullname: Fushiki – volume: 19 start-page: 589 year: 2000 ident: 10.1016/j.ejpb.2012.01.010_b0015 article-title: Characterization of mucoadhesive microspheres for the induction of mucosal and systemic immune responses publication-title: Vaccine doi: 10.1016/S0264-410X(00)00094-3 contributor: fullname: Kunisawa – volume: 56 start-page: 207 year: 2003 ident: 10.1016/j.ejpb.2012.01.010_b0055 article-title: Thiolated polymers: evidence for the formation of disulphide bonds with mucus glycoproteins publication-title: Eur. J. Pharm. Biopharm. doi: 10.1016/S0939-6411(03)00061-4 contributor: fullname: Leitner – volume: 325 start-page: 147 year: 2006 ident: 10.1016/j.ejpb.2012.01.010_b0030 article-title: Cyclodextrin–insulin complex encapsulated polymethacrylic acid based nanoparticles for oral insulin delivery publication-title: Int. J. Pharm. doi: 10.1016/j.ijpharm.2006.06.019 contributor: fullname: Sajeesh – volume: 335 start-page: 147 year: 2007 ident: 10.1016/j.ejpb.2012.01.010_b0045 article-title: Low molecular weight heparin loaded pH-sensitive microparticles publication-title: Int. J. Pharm. doi: 10.1016/j.ijpharm.2006.11.014 contributor: fullname: Meissner – volume: 79 start-page: 226 year: 2011 ident: 10.1016/j.ejpb.2012.01.010_b0115 article-title: Induction of Th1 polarized immune responses by thiolated eudragit-coated F4 and F18 fimbriae of enterotoxigenic Escherichia coli publication-title: Eur. J. Pharm. Biopharm. doi: 10.1016/j.ejpb.2011.04.016 contributor: fullname: Lee – volume: 26 start-page: 688 year: 1987 ident: 10.1016/j.ejpb.2012.01.010_b0160 article-title: Intramolecular disulfide loop formation in a peptide containing two cysteines publication-title: Biochemistry doi: 10.1021/bi00377a005 contributor: fullname: Snyder – volume: 146 start-page: 59 year: 1989 ident: 10.1016/j.ejpb.2012.01.010_b0175 article-title: Biodegradable microspheres: vaccine delivery system for oral immunization publication-title: Curr. Top Microbiol. Immunol. doi: 10.1007/978-3-642-74529-4_6 contributor: fullname: Eldridge – volume: 53 start-page: 193 year: 2006 ident: 10.1016/j.ejpb.2012.01.010_b0050 article-title: Development and characterization of new insulin containing polysaccharide nanoparticles publication-title: Colloids Surf. B. Biointerfaces doi: 10.1016/j.colsurfb.2006.09.012 contributor: fullname: Sarmento |
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| Snippet | To investigate the efficay of thiolated eudragit microspheres (TEMS) as an oral vaccine delivery system, F4 and F18 in E.coli were loaded in TEMS and orally... A vaccine delivery system based on thiolated eudragit microsphere (TEMS) was studied in vivo for its ability to elicit mucosal immunity against enterotoxigenic... |
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| SubjectTerms | Administration, Oral Animals Antigens, Bacterial - administration & dosage Antigens, Bacterial - immunology Biological and medical sciences Delivery system Enterotoxigenic Escherichia coli - immunology Escherichia coli Escherichia coli Infections - immunology Escherichia coli Proteins - administration & dosage Escherichia coli Proteins - immunology Female Fimbriae Proteins - administration & dosage Fimbriae Proteins - immunology General pharmacology Immune response Immunity, Mucosal Immunoglobulin A - immunology Immunoglobulin G - immunology In vivo test Medical sciences Mice Mice, Inbred ICR Microsphere Microspheres Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Polymethacrylic Acids - chemistry Sulfhydryl Compounds - chemistry Vaccines - administration & dosage Vaccines - immunology |
| Title | Efficacy of thiolated eudragit microspheres as an oral vaccine delivery system to induce mucosal immunity against enterotoxigenic Escherichia coli in mice |
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