Calorimetry-based profiling of blood plasma from colorectal cancer patients

• Published in: Biochimica et biophysica acta Vol. 1820; no. 12; pp. 1879 - 1885
• Main Authors: Todinova, Svetla, Krumova, Sashka, Kurtev, Panayot, Dimitrov, Valentin, Djongov, Lachezar, Dudunkov, Zlate, Taneva, Stefka G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2012
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor - blood; Blood plasma; blood proteins; Blood Proteins - metabolism; Calorimetry, Differential Scanning; Case-Control Studies; Colon - metabolism; Colorectal cancer; colorectal neoplasms; Colorectal Neoplasms - blood; Colorectal Neoplasms - classification; Colorectal Neoplasms - diagnosis; Differential scanning calorimetry; disease diagnosis; enthalpy; Excess heat capacity; Female; heat; Humans; inflammation; Inflammation - blood; Inflammation - diagnosis; Male; Middle Aged; monitoring; Neoplasm Staging; patients; phase transition; Plasma - metabolism; protein folding; proteome; Proteome - analysis; Rectum - metabolism; stomach neoplasms; temperature; Thermal transition; Thermodynamics; Transition temperature; Excess heat capacity; Differential scanning calorimetry; Transition temperature; Thermal transition; Colorectal cancer; STI; cPex; Blood plasma; TNM; ΔHcal; DSC; CRC; Tmax; TFM; GC

Differential scanning calorimetry (DSC), a highly sensitive technique for resolving thermally-induced protein folding/unfolding transitions, recently was recognized as a novel tool for disease diagnosis and monitoring. To further elaborate this approach we have applied DSC in a study of blood plasma...