Identification and IVC of spermatogonial stem cells in prepubertal buffaloes

• Published in: Theriogenology Vol. 81; no. 9; pp. 1312 - 1322
• Main Authors: Yu, Xue, Riaz, Hasan, Dong, Ping, Chong, Zhenlu, Luo, Xuan, Liang, Aixin, Yang, Liguo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2014
• Subjects: Adult Stem Cells - physiology; Animals; Biomarkers; Buffalo; buffaloes; Buffaloes - physiology; CDH1; Cdh1 Proteins - genetics; Cdh1 Proteins - metabolism; Cell Culture Techniques; Dolichos; flow cytometry; Gene Expression Regulation; genetic engineering; growth factors; immunohistochemistry; Male; messenger RNA; Mice; Proliferation; protein isoforms; receptor protein-tyrosine kinase; RNA, Messenger - genetics; RNA, Messenger - metabolism; seminiferous tubules; Sexual Maturation - physiology; somatic cells; spermatogonia; Spermatogonial stem cell; stem cells; Testis - cytology; Testis - physiology; Transplantation, Heterologous; Spermatogonial stem cell; Proliferation; CDH1; Buffalo

Development of suitable selective marker for buffalo spermatogonial stem cells (SSCs), optimization of long-term IVC conditions, and their pluripotent retention capacity in buffaloes can be of prime importance in selective genetic modifications of this species. In the present study, we identified CD...