Recognition of a Single Transmembrane Degron by Sequential Quality Control Checkpoints

• Published in: Molecular biology of the cell Vol. 14; no. 3; pp. 1268 - 1278
• Main Authors: Fayadat, Laurence, Kopito, Ron R.
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 01.03.2003
• Subjects: Amino Acid Sequence; Animals; Cell Line; Cell Membrane - metabolism; Cysteine Endopeptidases - metabolism; Disulfides - chemistry; Exocytosis - physiology; Hemagglutinin Glycoproteins, Influenza Virus - chemistry; Hemagglutinin Glycoproteins, Influenza Virus - metabolism; Humans; Lysosomes - metabolism; Membrane Proteins - chemistry; Membrane Proteins - metabolism; Multienzyme Complexes - metabolism; Proteasome Endopeptidase Complex; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Receptors, Antigen, T-Cell - chemistry; Receptors, Antigen, T-Cell - genetics; Receptors, Antigen, T-Cell - metabolism

To understand the relationship between conformational maturation and quality control–mediated proteolysis in the secretory pathway, we engineered the well-characterized degron from the α-subunit of the T-cell antigen receptor (TCRα) into the α-helical transmembrane domain of homotrimeric type I inte...