Supercritical Fluids Are Superior Media for Catalysis by Cross-Linked Enzyme Microcrystals of Subtilisin Carlsberg
We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. A...
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| Published in | Biotechnology progress Vol. 17; no. 2; pp. 355 - 358 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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American Chemical Society
2001
American Institute of Chemical Engineers |
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| Abstract | We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO2, especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme. |
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| AbstractList | Abstract
We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO
2
, especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme. We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC-ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO sub(2), especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme. We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC-ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO(2), especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme. We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO2, especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme. |
| Author | Barreiros, Susana Garcia, Silvia Almeida, M. Conceição Fontes, Nuno Peres, Célia Partridge, Johann Halling, Peter J. |
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| Cites_doi | 10.1002/(SICI)1097-0290(19970220)53:4<351::AID-BIT1>3.0.CO;2-M 10.1021/cr970040u 10.1073/pnas.89.3.1100 10.1021/ie970933c 10.1021/jo9618334 10.1021/ja972635c 10.1002/aic.690010222 10.1016/S0167-4838(98)00086-7 10.1021/ja961596m 10.1073/pnas.95.22.12918 10.1007/978-3-662-07380-3 10.1016/S0141-0229(97)00241-X 10.1073/pnas.94.9.4250 10.1016/0167-4838(94)90203-8 10.3109/07388559509150531 10.1002/bit.260391114 10.1039/a800408k 10.1073/pnas.92.24.10969 10.1016/S0141-0229(96)00204-9 10.1002/(SICI)1097-0290(19970720)55:2<367::AID-BIT14>3.0.CO;2-E 10.1016/S0167-7799(99)01322-0 10.1021/ja00044a064 10.1021/ja961383x |
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| Keywords | Peptidases Serine endopeptidases Crosslinked state Enzyme Reaction medium Supercritical solvent Hydrolases Enzymatic catalysis Subtilisin Microcrystal |
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| References | Xu, K.; Klibanov, A. M. PH control of the catalytic activity of cross-linked enzyme crystals in organic solvents. J. Am. Chem. Soc. 1996, 118, 9815-9819. (a) organic-soluble buffers or by (b) solid-state buffers. (a): Blackwood, A. D.; Curran, L. J.; Moore, B. D.; Halling, P. J. Organic phase buffers control biocatalyst activity independent of initial aqueous pH. Biochim. Biophys. Acta 1994, 1206, 161-1665. Griebenow, K.; Klibanov, A. M. Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents? Biotechnol. Bioeng. 1997, 53, 351-362. Affleck, R.; Xu, Z. F.; Suzawa, V.; Focht, K.; Clark, D. S.; Dordick, J. S. Enzymatic catalysis and dynamics in low-water environments. Proc. Natl. Acad. Sci. U.S.A. 1992, 89, 1100-1104. For reports on the impact of lyophilization on the secondary structure of proteins, see: Griebenow, K.; Klibanov, A. M. Lyophilization-induced reversible changes in the secondary structure of proteins. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 10969-10976. St. Clair, N. L.; Navia, M. A. Cross-linked enzyme crystals as robust catalysts. J. Am. Chem. Soc. 1992, 114, 7314-7316. Roziewski, K.; Russell, A. J. Effect of hydration on the morphology of enzyme powder. Biorechnol. Bioeng. 1992, 39, 1171-1175. (a) Mesiano, A. J.; Beckman, E. J.; Russell, A. J. Supercritical biocatalysis. Chem. Rev. 1999, 99, 623-633. (b) Almeida, M. C.; Ruivo, R.; Maia, C.; Freire, L.; Correa De Sampaio, T.; Barreiros, S. Novozym 435 Activity in compressed gases. Water activity and temperature effects. Enzyme Microb. Technol. 1998, 22, 494-499. Kamat, S. V.; Beckman, E. J.; Russell, A. J. Enzyme activity in supercritical fluids. Crit. Rev. Biotechnol. 1995, 15, 41-71. Wang, Y.-F.; Yakovlevsky, K.; Zhang, B.; Margolin, A. L. Cross-linked crystals of subtilisin: versatile catalyst for organic synthesis. J. Org. Chem. 1997, 62, 3488-3495. Tischer, W.; Kasche, V. Immobilized enzymes: crystals or carriers? Trends Biotechnol. 1999, 17, 326-335. Partridge, J.; Hutcheon, G. A.; Moore, B. D.; Halling, P. J. Exploiting hydration hysteresis for high activitv of cross-linked subtilisin crystals in acetonitrile. J. Am. Chem. Soc. 1996, 118, 12873-12877. Schmitke, J. L.; Stem, L. J.; Klibanov, A. M. Comparison of X-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 12918-12923. Partridge, J.; Dennison, P. R.; Moore, B. D.; Halling, P. J. Activity and mobility of subtilisin in low water organic media: hydration is more important than solvent dielectric. Biochim. Biophys. Acta 1998, 1386, 79-89. (b): Zacharis, E.; Moore, B. D.; Hailing, P. J. Control of enzyme activity in organic media by solid-state acid-base buffers. J. Am. Chem. Soc. 1997, 119, 12396-12397. See Bailey, J. E.; Ollis, D. F. Biochemical Engineering Fundamentals, 2nd ed.; McGraw-Hill: NY, 1986. Brunner, G. Gas Extraction: An Introduction to Fundamentals of Supercritical Fluids and the Application to Separation Processes; Springer-Verlag: NY, 1994. Schmitke, J. L.; Stem, L. J.; Klibanov, A. M. The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 4250-4255. (c) Fontes, N.; Almeida, M.; Conceicao Peres, C.; Garcia, S.; Grave, J.; Aires-Barros, M. R.; Soares, C. M.; Cabral, J. M. S.; Maycock, C. D.; Barreiros, S. Cutinase activity and enantioselectivity in supercritical fluids. Ind. Eng. Chem. Res. 1998, 37, 3189-3194. Zacharis, E.; Omar, I. C.; Partridge, J.; Robb, D. A.; Halling, P. J. Selection of salt hydrate pairs for use in water control in enzyme catalysis in organic solvents. Biotechnol. Bioeng. 1997, 55, 367-374. Wilke, C. R.; Chang, P. Correlation of diffusion coefficients in dilute solutions. AIChE J. 1955, 1, 264-270. In polar solvents such as acetonitrile, a fixed aw may be achieved by adding a known concentration of water; see: Bell, G.; Janssen, A. E. M.; Hailing, P. J. Water activity fails to predict critical hydration level for enzyme activity in polar organic solvents: interconversion of water concentrations and activities. Enzyme Microb. Technol. 1997, 20, 471-477. 1998; 37 1995 1997; 92 53 1997; 62 1997; 94 1997; 55 1995; 15 1997; 20 1999; 17 1992; 114 1994 1997; 1206 119 1992; 39 1998 1986 1999; 99 1994 1998; 95 1992; 89 1998; 22 1996; 118 1955; 1 1998; 1386 e_1_2_5_26_2 e_1_2_5_23_3 e_1_2_5_24_2 e_1_2_5_23_4 e_1_2_5_25_2 e_1_2_5_22_2 e_1_2_5_23_2 e_1_2_5_20_2 e_1_2_5_20_3 e_1_2_5_21_2 Bailey J. E. (e_1_2_5_17_2) 1986 e_1_2_5_14_2 e_1_2_5_13_2 e_1_2_5_9_2 e_1_2_5_16_2 e_1_2_5_8_2 e_1_2_5_15_2 e_1_2_5_7_2 e_1_2_5_10_2 e_1_2_5_6_2 e_1_2_5_5_2 e_1_2_5_12_2 e_1_2_5_4_2 e_1_2_5_11_2 e_1_2_5_3_2 e_1_2_5_2_2 e_1_2_5_17_3 e_1_2_5_18_2 e_1_2_5_19_2 |
| References_xml | – volume: 62 start-page: 3488 year: 1997 end-page: 3495 article-title: Cross‐linked crystals of subtilisin: versatile catalyst for organic synthesis publication-title: J. Org. Chem. – volume: 118 start-page: 12873 year: 1996 end-page: 12877 article-title: Exploiting hydration hysteresis for high activitv of cross‐linked subtilisin crystals in acetonitrile publication-title: J. Am. Chem. Soc. – volume: 89 start-page: 1100 year: 1992 end-page: 1104 article-title: Enzymatic catalysis and dynamics in low‐water environments publication-title: Proc. Natl. Acad. Sci. U.S.A. – year: 1986 – start-page: 841 year: 1998 end-page: 842 – volume: 99 start-page: 623 year: 1999 end-page: 633 article-title: Supercritical biocatalysis publication-title: Chem. Rev. – volume: 20 start-page: 471 year: 1997 end-page: 477 article-title: Water activity fails to predict critical hydration level for enzyme activity in polar organic solvents: interconversion of water concentrations and activities publication-title: Enzyme Microb. Technol. – volume: 37 start-page: 3189 year: 1998 end-page: 3194 article-title: Cutinase activity and enantioselectivity in supercritical fluids publication-title: Ind. Eng. Chem. Res. – volume: 55 start-page: 367 year: 1997 end-page: 374 article-title: Selection of salt hydrate pairs for use in water control in enzyme catalysis in organic solvents publication-title: Biotechnol. Bioeng. – volume: 1386 start-page: 79 year: 1998 end-page: 89 article-title: Activity and mobility of subtilisin in low water organic media: hydration is more important than solvent dielectric publication-title: Biochim. Biophys. Acta – volume: 22 start-page: 494 year: 1998 end-page: 499 article-title: Novozym 435 Activity in compressed gases. Water activity and temperature effects publication-title: Enzyme Microb. Technol. – volume: 95 start-page: 12918 year: 1998 end-page: 12923 article-title: Comparison of X‐ray crystal structures of an acyl‐enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water publication-title: Proc. Natl. Acad. Sci. U.S.A. – volume: 118 start-page: 9815 year: 1996 end-page: 9819 article-title: PH control of the catalytic activity of cross‐linked enzyme crystals in organic solvents publication-title: J. Am. Chem. Soc. – volume: 94 start-page: 4250 year: 1997 end-page: 4255 article-title: The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile publication-title: Proc. Natl. Acad. Sci. U.S.A. – volume: 1206 119 start-page: 161 12396 year: 1994 1997 end-page: 1665 12397 article-title: Organic phase buffers control biocatalyst activity independent of initial aqueous pH. Control of enzyme activity in organic media by solid‐state acid–base buffers publication-title: Biochim. Biophys. Acta J. Am. Chem. Soc. – volume: 1 start-page: 264 year: 1955 end-page: 270 article-title: Correlation of diffusion coefficients in dilute solutions publication-title: AIChE J. – volume: 92 53 start-page: 10969 351 year: 1995 1997 end-page: 10976 362 article-title: Lyophilization‐induced reversible changes in the secondary structure of proteins Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents? publication-title: Proc. Natl. Acad. Sci. U.S.A. Biotechnol. Bioeng. – volume: 17 start-page: 326 year: 1999 end-page: 335 article-title: Immobilized enzymes: crystals or carriers? publication-title: Trends Biotechnol. – volume: 15 start-page: 41 year: 1995 end-page: 71 article-title: Enzyme activity in supercritical fluids publication-title: Crit. Rev. Biotechnol. – year: 1994 – volume: 114 start-page: 7314 year: 1992 end-page: 7316 article-title: Cross‐linked enzyme crystals as robust catalysts publication-title: J. Am. Chem. Soc. – volume: 39 start-page: 1171 year: 1992 end-page: 1175 article-title: Effect of hydration on the morphology of enzyme powder publication-title: Biorechnol. Bioeng. – ident: e_1_2_5_20_3 doi: 10.1002/(SICI)1097-0290(19970220)53:4<351::AID-BIT1>3.0.CO;2-M – ident: e_1_2_5_6_2 doi: 10.1021/cr970040u – ident: e_1_2_5_14_2 doi: 10.1073/pnas.89.3.1100 – ident: e_1_2_5_8_2 doi: 10.1021/ie970933c – ident: e_1_2_5_5_2 – ident: e_1_2_5_4_2 doi: 10.1021/jo9618334 – ident: e_1_2_5_10_2 – ident: e_1_2_5_23_4 doi: 10.1021/ja972635c – ident: e_1_2_5_25_2 doi: 10.1002/aic.690010222 – ident: e_1_2_5_17_3 – ident: e_1_2_5_21_2 – ident: e_1_2_5_15_2 doi: 10.1016/S0167-4838(98)00086-7 – volume-title: Biochemical Engineering Fundamentals year: 1986 ident: e_1_2_5_17_2 contributor: fullname: Bailey J. 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| Snippet | We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity... We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity... Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic... |
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| SubjectTerms | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Catalysis Crystallization Esterification Fundamental and applied biological sciences. Psychology Kinetics Methods. Procedures. Technologies Subtilisins - chemistry Subtilisins - metabolism |
| Title | Supercritical Fluids Are Superior Media for Catalysis by Cross-Linked Enzyme Microcrystals of Subtilisin Carlsberg |
| URI | https://api.istex.fr/ark:/67375/WNG-GDGWDK9R-K/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1021%2Fbp000148m https://www.ncbi.nlm.nih.gov/pubmed/11312714 https://search.proquest.com/docview/17867379 https://search.proquest.com/docview/77075811 |
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