Supercritical Fluids Are Superior Media for Catalysis by Cross-Linked Enzyme Microcrystals of Subtilisin Carlsberg

We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. A...

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Published inBiotechnology progress Vol. 17; no. 2; pp. 355 - 358
Main Authors Fontes, Nuno, Almeida, M. Conceição, Garcia, Silvia, Peres, Célia, Partridge, Johann, Halling, Peter J., Barreiros, Susana
Format Journal Article
LanguageEnglish
Published USA American Chemical Society 2001
American Institute of Chemical Engineers
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Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO2, especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme.
AbstractList Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO 2 , especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme.
We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC-ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO sub(2), especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.
We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC-ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO(2), especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.
We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO2, especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme.
Author Barreiros, Susana
Garcia, Silvia
Almeida, M. Conceição
Fontes, Nuno
Peres, Célia
Partridge, Johann
Halling, Peter J.
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Issue 2
Keywords Peptidases
Serine endopeptidases
Crosslinked state
Enzyme
Reaction medium
Supercritical solvent
Hydrolases
Enzymatic catalysis
Subtilisin
Microcrystal
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Snippet We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity...
We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity...
Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic...
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SubjectTerms Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Catalysis
Crystallization
Esterification
Fundamental and applied biological sciences. Psychology
Kinetics
Methods. Procedures. Technologies
Subtilisins - chemistry
Subtilisins - metabolism
Title Supercritical Fluids Are Superior Media for Catalysis by Cross-Linked Enzyme Microcrystals of Subtilisin Carlsberg
URI https://api.istex.fr/ark:/67375/WNG-GDGWDK9R-K/fulltext.pdf
https://onlinelibrary.wiley.com/doi/abs/10.1021%2Fbp000148m
https://www.ncbi.nlm.nih.gov/pubmed/11312714
https://search.proquest.com/docview/17867379
https://search.proquest.com/docview/77075811
Volume 17
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