An Intracellular Endonuclease of Bacillus subtilis Specific for Single‐Stranded DNA

• Published in: European journal of biochemistry Vol. 61; no. 2; pp. 487 - 492
• Main Authors: CIARROCCHI, Giovanni, FORTUNATO, Adriana, COBIANCHI, Fabio, FALASCHI, Arturo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1976
• Subjects: Bacillus subtilis - enzymology; Calcium - pharmacology; Chloromercuribenzoates - pharmacology; Deoxyribonucleases - isolation & purification; Deoxyribonucleases - metabolism; Endonucleases - isolation & purification; Endonucleases - metabolism; Enzyme Activation - drug effects; Hydrogen-Ion Concentration; Isoenzymes - analysis; Isoenzymes - metabolism; Kinetics; Magnesium - pharmacology; Molecular Weight; Osmolar Concentration; Potassium Chloride - pharmacology

We have fractionated from extracts of Bacillus subtilis the DNase activity specific for singlestranded DNA; the activity separates in two main fractions on Sephadex G‐200, a larger one (Mr < 400000) and a smaller one (Mr∼ 30000). We have purified the smaller, more abundant fraction nearly 3000‐fo...