Actin polymerization in neutrophils from donors of peripheral blood stem cells: Divergent effects of glycosylated and nonglycosylated recombinant human granulocyte colony‐stimulating factor
Neutrophil functions can be modified by Recombinant human G‐CSF (rhG‐CSF) treatment, with divergent effects on phagocytosis, motility, bactericidal activity, and surface molecule expression. Neutrophil morphology is modified by treatment with filgrastim (the nonglycosylated form of rhG‐CSF), while i...
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| Published in | American journal of hematology Vol. 81; no. 5; pp. 318 - 323 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.05.2006
Wiley-Liss |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0361-8609 1096-8652 |
| DOI | 10.1002/ajh.20604 |
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| Abstract | Neutrophil functions can be modified by Recombinant human G‐CSF (rhG‐CSF) treatment, with divergent effects on phagocytosis, motility, bactericidal activity, and surface molecule expression. Neutrophil morphology is modified by treatment with filgrastim (the nonglycosylated form of rhG‐CSF), while it is not affected by lenograstim (the glycosylated type of rhG‐CSF). Little information is available about actin polymerization in neutrophils from subjects treated with the two types of rhG‐CSF. In the current paper we evaluated two groups of donors of peripheral blood stem cells (PBSC) for allogeneic transplantation. Ten subjects were treated with filgrastim and 10 with lenograstim to mobilize PBSC; 15 blood donors were evaluated as a control group. Actin polymerization (both spontaneous and fMLP‐stimulated) was studied by a flow cytometric assay. A microscopic fluorescent assay was also carried out to evaluate F‐actin distribution in neutrophils. We found that filgrastim induced an increased F‐actin content in resting neutrophils, along with morphologic evidence for increased actin polymerization distributed principally at the cell membrane and frequently polarized in focal areas; in addition, fMLP was not able to induce further actin polymerization. On the contrary, treatment with lenograstim was associated with F‐actin content, distribution, and polymerization kinetics indistinguishable from those displayed by control neutrophils. Such experimental results show that filgrastim and lenograstim display divergent effects also on neutrophil actin polymerization and provide further explanation for previous experimental findings. Am. J. Hematol. 81:318–323, 2006. © 2006 Wiley‐Liss, Inc. |
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| AbstractList | Neutrophil functions can be modified by Recombinant human G-CSF (rhG-CSF) treatment, with divergent effects on phagocytosis, motility, bactericidal activity, and surface molecule expression. Neutrophil morphology is modified by treatment with filgrastim (the nonglycosylated form of rhG-CSF), while it is not affected by lenograstim (the glycosylated type of rhG-CSF). Little information is available about actin polymerization in neutrophils from subjects treated with the two types of rhG-CSF. In the current paper we evaluated two groups of donors of peripheral blood stem cells (PBSC) for allogeneic transplantation. ten subjects were treated with filgrastim and 10 with lenograstim to mobilize PBSC; 15 blood donors were evaluated as a control group. Actin polymerization (both spontaneous and fMLP-stimulated) was studied by a flow cytometric assay. A microscopic fluorescent assay was also carried out to evaluate F-actin distribution in neutrophils. We found that filgrastim induced an increased F- actin content in resting neutrophils, along with morphologic evidence for increased actin polymerization distributed principally at the cell membrane and frequently polarized in focal areas; in addition, fMLP was not able to induce further actin polymerization. On the contrary, treatment with lenograstim was associated with F-actin content, distribution, and polymerization kinetics indistinguishable from those displayed by control neutrophils. Such experimental results show that filgrastim and lenograstim display divergent effects also on neutrophil actin polymerization and provide further explanation for previous experimental findings. Am. J. Hematol. 81:318-323, 2006. Neutrophil functions can be modified by Recombinant human G-CSF (rhG-CSF) treatment, with divergent effects on phagocytosis, motility, bactericidal activity, and surface molecule expression. Neutrophil morphology is modified by treatment with filgrastim (the nonglycosylated form of rhG-CSF), while it is not affected by lenograstim (the glycosylated type of rhG-CSF). Little information is available about actin polymerization in neutrophils from subjects treated with the two types of rhG-CSF. In the current paper we evaluated two groups of donors of peripheral blood stem cells (PBSC) for allogeneic transplantation. Ten subjects were treated with filgrastim and 10 with lenograstim to mobilize PBSC; 15 blood donors were evaluated as a control group. Actin polymerization (both spontaneous and fMLP-stimulated) was studied by a flow cytometric assay. A microscopic fluorescent assay was also carried out to evaluate F-actin distribution in neutrophils. We found that filgrastim induced an increased F-actin content in resting neutrophils, along with morphologic evidence for increased actin polymerization distributed principally at the cell membrane and frequently polarized in focal areas; in addition, fMLP was not able to induce further actin polymerization. On the contrary, treatment with lenograstim was associated with F-actin content, distribution, and polymerization kinetics indistinguishable from those displayed by control neutrophils. Such experimental results show that filgrastim and lenograstim display divergent effects also on neutrophil actin polymerization and provide further explanation for previous experimental findings.Neutrophil functions can be modified by Recombinant human G-CSF (rhG-CSF) treatment, with divergent effects on phagocytosis, motility, bactericidal activity, and surface molecule expression. Neutrophil morphology is modified by treatment with filgrastim (the nonglycosylated form of rhG-CSF), while it is not affected by lenograstim (the glycosylated type of rhG-CSF). Little information is available about actin polymerization in neutrophils from subjects treated with the two types of rhG-CSF. In the current paper we evaluated two groups of donors of peripheral blood stem cells (PBSC) for allogeneic transplantation. Ten subjects were treated with filgrastim and 10 with lenograstim to mobilize PBSC; 15 blood donors were evaluated as a control group. Actin polymerization (both spontaneous and fMLP-stimulated) was studied by a flow cytometric assay. A microscopic fluorescent assay was also carried out to evaluate F-actin distribution in neutrophils. We found that filgrastim induced an increased F-actin content in resting neutrophils, along with morphologic evidence for increased actin polymerization distributed principally at the cell membrane and frequently polarized in focal areas; in addition, fMLP was not able to induce further actin polymerization. On the contrary, treatment with lenograstim was associated with F-actin content, distribution, and polymerization kinetics indistinguishable from those displayed by control neutrophils. Such experimental results show that filgrastim and lenograstim display divergent effects also on neutrophil actin polymerization and provide further explanation for previous experimental findings. Neutrophil functions can be modified by Recombinant human G‐CSF (rhG‐CSF) treatment, with divergent effects on phagocytosis, motility, bactericidal activity, and surface molecule expression. Neutrophil morphology is modified by treatment with filgrastim (the nonglycosylated form of rhG‐CSF), while it is not affected by lenograstim (the glycosylated type of rhG‐CSF). Little information is available about actin polymerization in neutrophils from subjects treated with the two types of rhG‐CSF. In the current paper we evaluated two groups of donors of peripheral blood stem cells (PBSC) for allogeneic transplantation. Ten subjects were treated with filgrastim and 10 with lenograstim to mobilize PBSC; 15 blood donors were evaluated as a control group. Actin polymerization (both spontaneous and fMLP‐stimulated) was studied by a flow cytometric assay. A microscopic fluorescent assay was also carried out to evaluate F‐actin distribution in neutrophils. We found that filgrastim induced an increased F‐actin content in resting neutrophils, along with morphologic evidence for increased actin polymerization distributed principally at the cell membrane and frequently polarized in focal areas; in addition, fMLP was not able to induce further actin polymerization. On the contrary, treatment with lenograstim was associated with F‐actin content, distribution, and polymerization kinetics indistinguishable from those displayed by control neutrophils. Such experimental results show that filgrastim and lenograstim display divergent effects also on neutrophil actin polymerization and provide further explanation for previous experimental findings. Am. J. Hematol. 81:318–323, 2006. © 2006 Wiley‐Liss, Inc. Neutrophil functions can be modified by Recombinant human G-CSF (rhG-CSF) treatment, with divergent effects on phagocytosis, motility, bactericidal activity, and surface molecule expression. Neutrophil morphology is modified by treatment with filgrastim (the nonglycosylated form of rhG-CSF), while it is not affected by lenograstim (the glycosylated type of rhG-CSF). Little information is available about actin polymerization in neutrophils from subjects treated with the two types of rhG-CSF. In the current paper we evaluated two groups of donors of peripheral blood stem cells (PBSC) for allogeneic transplantation. Ten subjects were treated with filgrastim and 10 with lenograstim to mobilize PBSC; 15 blood donors were evaluated as a control group. Actin polymerization (both spontaneous and fMLP-stimulated) was studied by a flow cytometric assay. A microscopic fluorescent assay was also carried out to evaluate F-actin distribution in neutrophils. We found that filgrastim induced an increased F-actin content in resting neutrophils, along with morphologic evidence for increased actin polymerization distributed principally at the cell membrane and frequently polarized in focal areas; in addition, fMLP was not able to induce further actin polymerization. On the contrary, treatment with lenograstim was associated with F-actin content, distribution, and polymerization kinetics indistinguishable from those displayed by control neutrophils. Such experimental results show that filgrastim and lenograstim display divergent effects also on neutrophil actin polymerization and provide further explanation for previous experimental findings. |
| Author | Galimberti, Sara Benedetti, Edoardo Zucca, Alessandra Carulli, Giovanni Azzarà, Antonio Mattii, Letizia Brizzi, Stefania Petrini, Mario |
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| Cites_doi | 10.1016/S0022-1759(99)00168-4 10.1046/j.1365-2141.1996.00295.x 10.1002/ajh.1064 10.1016/S0955-0674(02)00310-1 10.1038/sj.bmt.1703136 10.1016/S0145-2126(97)00009-X 10.1002/jcb.20248 10.1182/blood.V81.10.2750.2750 10.1016/S1357-2725(03)00144-4 10.1177/154411130201300302 10.1182/blood.V92.11.4366 10.1111/j.1365-2362.1995.tb01969.x 10.1046/j.1365-2141.1997.d01-2029.x 10.1185/030079903125002531 10.2165/00003495-200059030-00017 10.1111/j.1365-2141.2004.05008.x 10.1097/00001813-200103000-00002 10.1083/jcb.101.3.1078 |
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| Keywords | Hematology rhG-CSF Granulocyte Hematopoietic cell Polymerization Contractile protein Glycosylation Human factor actin polymerization Granulocyte colony stimulating factor Actin Neutrophil Recombinant protein neutrophils Blood donor |
| Language | English |
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| SubjectTerms | actin actin polymerization Actins - metabolism Adult Antigens, CD34 - metabolism Biological and medical sciences Blood Donors Female Filgrastim Flow Cytometry Glycosylation Granulocyte Colony-Stimulating Factor - administration & dosage Granulocyte Colony-Stimulating Factor - pharmacology Granulocyte Colony-Stimulating Factor - therapeutic use Hematologic and hematopoietic diseases Humans Injections, Subcutaneous Leukocyte Count Male Medical sciences Microscopy, Fluorescence Middle Aged neutrophils Neutrophils - cytology Neutrophils - drug effects Neutrophils - metabolism Peripheral Blood Stem Cell Transplantation Recombinant Proteins - administration & dosage Recombinant Proteins - pharmacology Recombinant Proteins - therapeutic use rhG‐CSF |
| Title | Actin polymerization in neutrophils from donors of peripheral blood stem cells: Divergent effects of glycosylated and nonglycosylated recombinant human granulocyte colony‐stimulating factor |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fajh.20604 https://www.ncbi.nlm.nih.gov/pubmed/16628714 https://www.proquest.com/docview/19960651 https://www.proquest.com/docview/67907608 |
| Volume | 81 |
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