Identifying adeno‐associated virus (AAV) vectors that efficiently target high grade glioma cells, for in vitro monitoring of temporal cell responses

• Published in: FEBS open bio Vol. 14; no. 11; pp. 1914 - 1925
• Main Authors: Sarker, Farhana A., Chen, Yuyan, Westhaus, Adrian, Lisowski, Leszek, O'Neill, Geraldine M.
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2024; John Wiley and Sons Inc; Wiley
• Subjects: AAV; Bar codes; barcoded; Biotechnology; Brain Cancer; Brain Neoplasms - genetics; Brain Neoplasms - pathology; Brain tumors; Cell culture; Cell Line, Tumor; Cells; Dependovirus - genetics; Expression vectors; Genetic Vectors - genetics; Genomes; Genomics; Glioblastoma; Glioma; Glioma - genetics; Glioma - pathology; Glioma cells; High-Throughput Nucleotide Sequencing - methods; Humans; Invoices; Medical research; Next Generation Sequencing; Pediatrics; Phenotypes; Proteins; Transcription activation; Transcription factors; Tropism; Tumors; Viral Vector; Viruses; YAP/TAZ; Yes-associated protein; England; United Kingdom > UK; Australia; United States > US; Germany; glioma; barcoded; YAP/TAZ; AAV

To improve the translation of preclinical cancer research data to successful clinical effect, there is an increasing focus on the use of primary patient‐derived cancer cells with limited growth in culture to reduce genetic and phenotype drift. However, these primary lines are less amenable to standa...