Human Gene Mapping and Chromosome Sorting by Laser Technology

More than 30 human genes have been mapped by a combination of the fluorescent in situ hybridization (FISH) and spot blot hybridization in this laboratory for the past five years. Furthermore, the Keio strategy has been established for the molecular analysis of the human genome using flow-sorted huma...

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Bibliographic Details
Published inKeio journal of medicine Vol. 42; no. 4; pp. 212 - 216
Main Authors Kudoh, Jun, Wang, Yimin, Shimizu, Nobuyoshi, Sakai, Kosuke, Kawasaki, Kazuhiko, Shimizu, Yoshiko, Minoshima, Shinsei
Format Journal Article
LanguageEnglish
Published Japan The Keio Journal of Medicine 1993
Subjects
Base Sequence
Cell Separation - instrumentation
Chromosome Mapping - instrumentation
Chromosome Mapping - methods
Cosmids
flow cytometry
Flow Cytometry - instrumentation
Flow Cytometry - methods
fluorescent in situ hybridization
Gene Library
gene mapping and human genome
Genome, Human
Humans
Immunoblotting - instrumentation
Immunoblotting - methods
In Situ Hybridization, Fluorescence - instrumentation
In Situ Hybridization, Fluorescence - methods
Lasers
Molecular Sequence Data
Sequence Analysis, DNA - instrumentation
spot blot hybridization
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ISSN0022-9717
1880-1293
DOI10.2302/kjm.42.212

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Summary:More than 30 human genes have been mapped by a combination of the fluorescent in situ hybridization (FISH) and spot blot hybridization in this laboratory for the past five years. Furthermore, the Keio strategy has been established for the molecular analysis of the human genome using flow-sorted human chromosomes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-9717
1880-1293
DOI:10.2302/kjm.42.212