Cross-linking of CD23 antigen by its natural ligand (IgE) or by anti- CD23 antibody prevents B lymphocyte proliferation and differentiation

• Published in: The Journal of immunology (1950) Vol. 146; no. 7; pp. 2122 - 2129
• Main Authors: Luo, HY, Hofstetter, H, Banchereau, J, Delespesse, G
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.04.1991; American Association of Immunologists
• Subjects: Antibodies, Monoclonal; Antigens, Differentiation, B-Lymphocyte - metabolism; Antigens, Differentiation, B-Lymphocyte - physiology; B-Lymphocytes - cytology; B-Lymphocytes - immunology; Biological and medical sciences; Cell Differentiation; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Immunoglobulin E - metabolism; In Vitro Techniques; Lymphocyte Activation; Modulation of the immune response (stimulation, suppression); Receptor Aggregation; Receptors, Fc - metabolism; Receptors, IgE; Solubility; Human; Cell proliferation; IgE; Molecular interaction; Immune regulation; Monoclonal antibody; Soluble form; Fab-Fragment; Cell differentiation; In vitro; B»-Lymphocyte; Membrane receptor; Inhibition
• DOI: 10.4049/jimmunol.146.7.2122
• ISSN: 0022-1767

The possible role of CD23 in the activation of human B lymphocytes was systematically investigated by examining the effect of: 1) anti-CD23 mAb; 2) IgE or IgE-immune complexes and; 3) native or recombinant soluble CD23 of different m.w., on B cell proliferation. Intact anti-CD23 mAb or its F(ab')2 fragments inhibit the proliferation of tonsillar B lymphocytes costimulated with either Staphylococcus aureus Cowan I (SAC) or anti-IgM and IL-4. The antibody has no effect when IL-2 or LMW-BCGF is used as the second stimulant. The response of IL-4-pretreated B cells (expressing high levels of CD23) to anti-IgM together with IL-2 or B cell-derived B cell growth factor is inhibited by anti-CD23 mAb, indicating that this antibody prevents B cell activation regardless of the B cell activators but provided that the density of CD23 on B cells is sufficient. Anti-CD23 mAb markedly inhibits DNA synthesis only when added during the first 12 h of the culture and has no effect on the ongoing proliferation of CD23-bearing B cell blasts (SAC induced and IL-4 supported or EBV transformed). Monovalent Fab fragments of anti-CD23 mAb are inactive unless they are used in tandem with goat anti-mouse Fab suggesting that the inhibition is due to cross-linking of surface CD23. Most interestingly, polymeric IgE or IgE-immune complexes have the same effect as anti-CD23 and moreover they inhibit IgM production by SAC and IL4-stimulated B cells. The inhibiting effect of IgE or of anti-CD23 mAb is not due to their neutralization of soluble CD23 because these failed to display B cell growth factor activity under various experimental conditions. It is concluded that IgE-immune complexes may regulate activation and differentiation of CD23-bearing surfaceIgM/surfaceIgD precursor B lymphocytes.