Ultrastructural localization of adenylate cyclase activity in chicken osteoclasts

• Published in: The journal of histochemistry and cytochemistry Vol. 39; no. 9; pp. 1207 - 1213
• Main Authors: Fukushima, O, Yamamoto, T, Gay, CV
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Histochemical Soc 01.09.1991; SAGE Publications; Histochemical Society
• Subjects: Adenosine Triphosphate - analogs & derivatives; Adenosine Triphosphate - pharmacology; Adenylyl Cyclases - metabolism; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Calcium, Dietary - administration & dosage; Cell Membrane - enzymology; Chickens; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Guanosine Triphosphate - analogs & derivatives; Guanosine Triphosphate - pharmacology; Histocytochemistry; Osteoclasts - enzymology; Osteoclasts - ultrastructure; Adenylate cyclase; Vertebrata; Enzymatic activity; Calcium; Diet; Enzyme; Osteoclast; Chicken; Bone; Aves; Cytochemistry; Localization

Using lead citrate as a capture reagent and adenylate-(beta, gamma-methylene) diphosphate (AMP-PCP) as a substrate, we localized adenylate cyclase activity on the non-ruffled border plasma membrane of approximately half of the osteoclasts on trabecular bone surfaces in the tibial metaphyses of chick...