OXIDATIVE STRESS INDUCED BY HUMIC ACID SOLVENT EXTRACTION FRACTION IN CULTURED RABBIT ARTICULAR CHONDROCYTES

• Published in: Journal of Toxicology and Environmental Health, Part A Vol. 54; no. 6; pp. 477 - 489
• Main Authors: Liang, H J, Tsai, C L, Lu, F J
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 24.07.1998
• Subjects: Animals; Antioxidants - pharmacology; Cartilage, Articular - cytology; Cartilage, Articular - drug effects; Cartilage, Articular - metabolism; Cell Survival - drug effects; Cells, Cultured; Chelating Agents - chemistry; Chelating Agents - toxicity; Chondrocytes - drug effects; Chondrocytes - metabolism; Dose-Response Relationship, Drug; Hip Joint - cytology; Humic Substances - chemistry; Humic Substances - toxicity; Knee Joint - cytology; L-Lactate Dehydrogenase - metabolism; Luminescent Measurements; Malondialdehyde - metabolism; Oxidative Stress - drug effects; Rabbits; Solvents - chemistry; Tetrazolium Salts - metabolism

Kashin-Beck disease (KBD) , an endemic, chronic osteoarthritic disorder with necrosis of chondrocytes, commonly occurs in China. The humic substance present in the drinking water of endemic areas has been proposed as one of the causative factors. In this study an in vitro cell culture system was use...