Sub-lethal Doses of Polybrominated Diphenyl Ethers, in Vitro, Promote Oxidative Stress and Modulate Molecular Markers Related to Cell Cycle, Antioxidant Balance and Cellular Energy Management

In the present study, we evaluated the effects of different concentrations of the polybrominated diphenyl ethers (PBDEs) BDE-209, BDE-47 and BDE-99, on the vitality and oxidative stress of a HS-68 human cell culture exposed to the compounds for three days. The results showed that for this exposure t...

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Published inInternational journal of environmental research and public health Vol. 16; no. 4; p. 588
Main Authors Manuguerra, Simona, Espinosa Ruiz, Cristóbal, Santulli, Andrea, Messina, Concetta Maria
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 18.02.2019
MDPI
Subjects
Adenosine diphosphate
Adenosine kinase
Angiogenesis
Antioxidants
Antioxidants - metabolism
Apoptosis
Biomarkers - metabolism
Breast cancer
c-Fos protein
c-Jun protein
Cancer
Cell Cycle
Cell Line
Cell proliferation
Congeners
Cytotoxicity
Damage detection
Deoxyribonucleic acid
DNA
DNA damage
DNA repair
Early warning systems
Energy management
Energy Metabolism
Environmental Pollutants - toxicity
Enzymes
Estrogens
Ethers
Exposure
Extracellular signal-regulated kinase
Fibroblasts
GA-binding protein
Genomes
Halogenated Diphenyl Ethers - toxicity
Homeostasis
Humans
Hypoxia
Kinases
Marine environment
Metabolism
Oxidative stress
Oxidative Stress - drug effects
Oxidizing agents
Poly(ADP-ribose) polymerase
Polybrominated diphenyl ethers
Protein kinase
Protein-serine/threonine kinase
Proteins
Retina
Retinoblastoma
Ribose
Terrestrial environments
Thyroxine
Transcription factors
Tumor suppressor genes
United States > US
metabolism
proliferation
PBDEs
biomarkers
oxidative stress
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Abstract In the present study, we evaluated the effects of different concentrations of the polybrominated diphenyl ethers (PBDEs) BDE-209, BDE-47 and BDE-99, on the vitality and oxidative stress of a HS-68 human cell culture exposed to the compounds for three days. The results showed that for this exposure time, only the highest concentrations produced a significant vitality reduction and oxidative stress induction ( < 0.05), measured as reactive oxygen species (ROS). Subsequently, in order to verify the effects of sub-lethal doses, cells were exposed for a longer time and data collected, after 12 and 20 days, to study ROS production and some molecular markers related to cell cycle and stress (p53, pRB, PARP, c-Jun and c-Fos), antioxidant status and proliferation (ERK, c-Jun and c-Fos), energy balance (NRF2, AMPK, HIF). Most of the biomarkers were influenced by the treatments, indicating that sub-lethal doses of PBDEs, for longer time, can enhance the production of ROS, altering the energetic metabolism, cell cycle and antioxidant balance, determining possible negative effects on the cell proliferation equilibrium.
AbstractList In the present study, we evaluated the effects of different concentrations of the polybrominated diphenyl ethers (PBDEs) BDE-209, BDE-47 and BDE-99, on the vitality and oxidative stress of a HS-68 human cell culture exposed to the compounds for three days. The results showed that for this exposure time, only the highest concentrations produced a significant vitality reduction and oxidative stress induction ( < 0.05), measured as reactive oxygen species (ROS). Subsequently, in order to verify the effects of sub-lethal doses, cells were exposed for a longer time and data collected, after 12 and 20 days, to study ROS production and some molecular markers related to cell cycle and stress (p53, pRB, PARP, c-Jun and c-Fos), antioxidant status and proliferation (ERK, c-Jun and c-Fos), energy balance (NRF2, AMPK, HIF). Most of the biomarkers were influenced by the treatments, indicating that sub-lethal doses of PBDEs, for longer time, can enhance the production of ROS, altering the energetic metabolism, cell cycle and antioxidant balance, determining possible negative effects on the cell proliferation equilibrium.
In the present study, we evaluated the effects of different concentrations of the polybrominated diphenyl ethers (PBDEs) BDE-209, BDE-47 and BDE-99, on the vitality and oxidative stress of a HS-68 human cell culture exposed to the compounds for three days. The results showed that for this exposure time, only the highest concentrations produced a significant vitality reduction and oxidative stress induction ( p < 0.05), measured as reactive oxygen species (ROS). Subsequently, in order to verify the effects of sub-lethal doses, cells were exposed for a longer time and data collected, after 12 and 20 days, to study ROS production and some molecular markers related to cell cycle and stress (p53, pRB, PARP, c-Jun and c-Fos), antioxidant status and proliferation (ERK, c-Jun and c-Fos), energy balance (NRF2, AMPK, HIF). Most of the biomarkers were influenced by the treatments, indicating that sub-lethal doses of PBDEs, for longer time, can enhance the production of ROS, altering the energetic metabolism, cell cycle and antioxidant balance, determining possible negative effects on the cell proliferation equilibrium.
In the present study, we evaluated the effects of different concentrations of the polybrominated diphenyl ethers (PBDEs) BDE-209, BDE-47 and BDE-99, on the vitality and oxidative stress of a HS-68 human cell culture exposed to the compounds for three days. The results showed that for this exposure time, only the highest concentrations produced a significant vitality reduction and oxidative stress induction (p < 0.05), measured as reactive oxygen species (ROS). Subsequently, in order to verify the effects of sub-lethal doses, cells were exposed for a longer time and data collected, after 12 and 20 days, to study ROS production and some molecular markers related to cell cycle and stress (p53, pRB, PARP, c-Jun and c-Fos), antioxidant status and proliferation (ERK, c-Jun and c-Fos), energy balance (NRF2, AMPK, HIF). Most of the biomarkers were influenced by the treatments, indicating that sub-lethal doses of PBDEs, for longer time, can enhance the production of ROS, altering the energetic metabolism, cell cycle and antioxidant balance, determining possible negative effects on the cell proliferation equilibrium.
Author Espinosa Ruiz, Cristóbal
Santulli, Andrea
Manuguerra, Simona
Messina, Concetta Maria
AuthorAffiliation 1 Department of Earth and Sea Science, Laboratory of Marine Biochemistry and Ecotoxicology, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy; simona.manuguerra@unipa.it (S.M.); cristobal.espinosaruiz@unipa.it (C.E.R.); andrea.santulli@unipa.it (A.S.)
2 Marine Biology Institute, Consorzio Universitario della Provincia di Trapani, Via Barlotta 4, 91100 Trapani, Italy
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– name: 1 Department of Earth and Sea Science, Laboratory of Marine Biochemistry and Ecotoxicology, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy; simona.manuguerra@unipa.it (S.M.); cristobal.espinosaruiz@unipa.it (C.E.R.); andrea.santulli@unipa.it (A.S.)
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  organization: Department of Earth and Sea Science, Laboratory of Marine Biochemistry and Ecotoxicology, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy. simona.manuguerra@unipa.it
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  givenname: Concetta Maria
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  surname: Messina
  fullname: Messina, Concetta Maria
  email: concetta.messina@unipa.it
  organization: Department of Earth and Sea Science, Laboratory of Marine Biochemistry and Ecotoxicology, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy. concetta.messina@unipa.it
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Issue 4
Keywords metabolism
proliferation
PBDEs
biomarkers
oxidative stress
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Snippet In the present study, we evaluated the effects of different concentrations of the polybrominated diphenyl ethers (PBDEs) BDE-209, BDE-47 and BDE-99, on the...
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SubjectTerms Adenosine diphosphate
Adenosine kinase
Angiogenesis
Antioxidants
Antioxidants - metabolism
Apoptosis
Biomarkers - metabolism
Breast cancer
c-Fos protein
c-Jun protein
Cancer
Cell Cycle
Cell Line
Cell proliferation
Congeners
Cytotoxicity
Damage detection
Deoxyribonucleic acid
DNA
DNA damage
DNA repair
Early warning systems
Energy management
Energy Metabolism
Environmental Pollutants - toxicity
Enzymes
Estrogens
Ethers
Exposure
Extracellular signal-regulated kinase
Fibroblasts
GA-binding protein
Genomes
Halogenated Diphenyl Ethers - toxicity
Homeostasis
Humans
Hypoxia
Kinases
Marine environment
Metabolism
Oxidative stress
Oxidative Stress - drug effects
Oxidizing agents
Poly(ADP-ribose) polymerase
Polybrominated diphenyl ethers
Protein kinase
Protein-serine/threonine kinase
Proteins
Retina
Retinoblastoma
Ribose
Terrestrial environments
Thyroxine
Transcription factors
Tumor suppressor genes
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Title Sub-lethal Doses of Polybrominated Diphenyl Ethers, in Vitro, Promote Oxidative Stress and Modulate Molecular Markers Related to Cell Cycle, Antioxidant Balance and Cellular Energy Management
URI https://www.ncbi.nlm.nih.gov/pubmed/30781636
https://www.proquest.com/docview/2328974369/abstract/
https://search.proquest.com/docview/2184140115
https://pubmed.ncbi.nlm.nih.gov/PMC6406823
Volume 16
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