PCR amplification of repetitive sequences as a possible approach in relative species quantification

• Published in: Meat science Vol. 90; no. 2; pp. 438 - 443
• Main Authors: Ballin, N.Z., Vogensen, F.K., Karlsson, A.H.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2012; Elsevier
• Subjects: Animals; Authentication; Biological and medical sciences; Chickens; detection limit; DNA; DNA - isolation & purification; DNA primers; DNA Primers - genetics; Dogs; Food industries; Fundamental and applied biological sciences. Psychology; genome; Horses; Limit of Detection; LUX primers; Meat; Meat - analysis; Meat and meat product industries; polymerase chain reaction; Rabbits; Real time PCR; Real-Time Polymerase Chain Reaction - methods; Repetitive sequences; Repetitive Sequences, Nucleic Acid; Sheep; Species identification; Species Specificity; Swine; Real time PCR; Species identification; Authentication; Repetitive sequences; LUX primers; Meat; Meat product; Quantitative analysis

Both relative and absolute quantifications are possible in species quantification when single copy genomic DNA is used. However, amplification of single copy genomic DNA does not allow a limit of detection as low as one obtained from amplification of repetitive sequences. Amplification of repetitive...