PCR amplification of repetitive sequences as a possible approach in relative species quantification
Both relative and absolute quantifications are possible in species quantification when single copy genomic DNA is used. However, amplification of single copy genomic DNA does not allow a limit of detection as low as one obtained from amplification of repetitive sequences. Amplification of repetitive...
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| Published in | Meat science Vol. 90; no. 2; pp. 438 - 443 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Kidlington
Elsevier Ltd
01.02.2012
Elsevier |
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| Abstract | Both relative and absolute quantifications are possible in species quantification when single copy genomic DNA is used. However, amplification of single copy genomic DNA does not allow a limit of detection as low as one obtained from amplification of repetitive sequences. Amplification of repetitive sequences is therefore frequently used in absolute quantification but problems occur in relative quantification as the number of repetitive sequences is unknown. A promising approach was developed where data from amplification of repetitive sequences were used in relative quantification of species in binary mixtures. PCR LUX primers were designed that amplify repetitive and single copy sequences to establish the species dependent number (constants) (SDC) of amplified repetitive sequences per genome. The SDCs and data from amplification of repetitive sequences were tested for their applicability to relatively quantify the amount of chicken DNA in a binary mixture of chicken DNA and pig DNA. However, the designed PCR primers lack the specificity required for regulatory species control. |
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| AbstractList | Both relative and absolute quantifications are possible in species quantification when single copy genomic DNA is used. However, amplification of single copy genomic DNA does not allow a limit of detection as low as one obtained from amplification of repetitive sequences. Amplification of repetitive sequences is therefore frequently used in absolute quantification but problems occur in relative quantification as the number of repetitive sequences is unknown. A promising approach was developed where data from amplification of repetitive sequences were used in relative quantification of species in binary mixtures. PCR LUX primers were designed that amplify repetitive and single copy sequences to establish the species dependent number (constants) (SDC) of amplified repetitive sequences per genome. The SDCs and data from amplification of repetitive sequences were tested for their applicability to relatively quantify the amount of chicken DNA in a binary mixture of chicken DNA and pig DNA. However, the designed PCR primers lack the specificity required for regulatory species control. |
| Author | Ballin, N.Z. Vogensen, F.K. Karlsson, A.H. |
| Author_xml | – sequence: 1 givenname: N.Z. surname: Ballin fullname: Ballin, N.Z. email: nixb@fvst.dk, nicolaiba@hotmail.com organization: Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark – sequence: 2 givenname: F.K. surname: Vogensen fullname: Vogensen, F.K. organization: Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark – sequence: 3 givenname: A.H. surname: Karlsson fullname: Karlsson, A.H. organization: Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark |
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| CitedBy_id | crossref_primary_10_1016_j_foodchem_2019_125654 crossref_primary_10_1007_s12668_013_0080_y crossref_primary_10_1016_j_jprot_2013_01_029 crossref_primary_10_1016_j_foodres_2014_03_030 crossref_primary_10_1016_j_meatsci_2012_12_012 crossref_primary_10_21323_2414_438X_2019_4_4_23_27 crossref_primary_10_1007_s12161_014_9914_7 crossref_primary_10_1016_j_foodcont_2015_12_036 crossref_primary_10_1016_j_lwt_2014_10_049 crossref_primary_10_3390_microorganisms7080230 crossref_primary_10_1016_j_foodcont_2019_05_016 crossref_primary_10_19113_sdufenbed_442199 crossref_primary_10_1016_j_meatsci_2014_06_006 crossref_primary_10_1155_2021_7710462 |
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| Keywords | Real time PCR Species identification Authentication Repetitive sequences LUX primers Meat Meat product Quantitative analysis |
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| SubjectTerms | Animals Authentication Biological and medical sciences Chickens detection limit DNA DNA - isolation & purification DNA primers DNA Primers - genetics Dogs Food industries Fundamental and applied biological sciences. Psychology genome Horses Limit of Detection LUX primers Meat Meat - analysis Meat and meat product industries polymerase chain reaction Rabbits Real time PCR Real-Time Polymerase Chain Reaction - methods Repetitive sequences Repetitive Sequences, Nucleic Acid Sheep Species identification Species Specificity Swine |
| Title | PCR amplification of repetitive sequences as a possible approach in relative species quantification |
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