Molecular cloning of a soybean class III β-1,3-glucanase gene that is regulated both developmentally and in response to pathogen infection

• Published in: Plant science (Limerick) Vol. 154; no. 1; pp. 71 - 81
• Main Authors: Cheong, Yong Hwa, Kim, Cha Young, Chun, Hyun Jin, Moon, Byeong Cheol, Park, Hyeong Cheol, Kim, Jong Kyoung, Lee, Sung-Ho, Han, Chang-deok, Lee, Sang Yeol, Cho, Moo Je
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 15.05.2000
• Subjects: Abiotic stresses; amino acid sequences; b-1,3-glucanase; beta-glucanase; beta-glucuronidase; Class III β-1,3-glucanase; defense mechanisms; ethephon; genbank/u41323; genes; genetic regulation; Glycine max; GUS activity; hydrogen peroxide; infection; isozymes; jasmonic acid; mechanical damage; molecular sequence data; nucleic acid hybridization; nucleotide sequences; Pathogen infection; Phytophthora; plant development; Pseudomonas syringae; reporter genes; roots; salicylic acid; seedlings; SGN1 gene; transgenic plants; Wounding; Wounding; Abiotic stresses; Class III β-1,3-glucanase; Pathogen infection; GUS activity
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(00)00187-4

We isolated and characterized a soybean gene ( SGN1) encoding a basic β-1,3-glucanase that is a plant class III isoform of β-1,3-glucanase. The deduced amino acid sequence of the SGN1 gene is similar to that of the PR-Q′b gene, the basic class III β-1,3-glucanase of tomato. Based on RNA blot hybridization, SGN1 gene expression was detected in all tissues of 4-day old seedlings, but it was present only in root tissue of 30-day old plants. GUS expression analysis carried out in transgenic tobacco plants harboring a SGN1::GUS reporter gene revealed the same expression pattern. Furthermore, the expression of SGN1 was strongly induced by a variety of defense-related signals, such as treatment with H 2O 2, wounding, or treatment with fungal elicitor prepared from Phytophthora spp as well as inoculation with Pseudomonas syringae. However, the expression level of SGN1 was hardly induced with jasmonate, ethephon and salicylate. Overall the results suggest that the SGN1 may play a role in both plant development and plant defense against pathogen attack.