Giardia lamblia: a culture method for determining parasite viability
A simple, quantitative method has been developed to assess the viability of Giardia lamblia trophozoites after exposure to a potentially lethal agent. This method utilizes the ability of trophozoites which remain viable to replicate and multiply. In this study, the percent dead trophozoites after in...
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Published in | The American journal of tropical medicine and hygiene Vol. 35; no. 6; p. 1129 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.11.1986
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Abstract | A simple, quantitative method has been developed to assess the viability of Giardia lamblia trophozoites after exposure to a potentially lethal agent. This method utilizes the ability of trophozoites which remain viable to replicate and multiply. In this study, the percent dead trophozoites after incubation in 20% fresh human serum was determined first by morphologic criteria and then by the ability of parasites to multiply in fresh medium over 48 hr. Using the number of parasites after 48 hr of growth, the original number of dead trophozoites in the serum-exposed samples was determined by extrapolation from a standard growth curve. Ten sera which killed from 7% to 89% of trophozoites by morphologic criteria, killed 8% to 92% using the culture method; these two assays for viability correlated highly (r = 0.96). Use of the culture assay allows the assessment of the lethal effects of serum and can be applied both to the study of other host defense mechanisms on Giardia trophozoites and to antimicrobial susceptibility testing. |
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AbstractList | A simple, quantitative method has been developed to assess the viability of Giardia lamblia trophozoites after exposure to a potentially lethal agent. This method utilizes the ability of trophozoites which remain viable to replicate and multiply. In this study, the percent dead trophozoites after incubation in 20% fresh human serum was determined first by morphologic criteria and then by the ability of parasites to multiply in fresh medium over 48 hr. Using the number of parasites after 48 hr of growth, the original number of dead trophozoites in the serum-exposed samples was determined by extrapolation from a standard growth curve. Ten sera which killed from 7% to 89% of trophozoites by morphologic criteria, killed 8% to 92% using the culture method; these two assays for viability correlated highly (r = 0.96). Use of the culture assay allows the assessment of the lethal effects of serum and can be applied both to the study of other host defense mechanisms on Giardia trophozoites and to antimicrobial susceptibility testing. |
Author | Pohl, R Pearson, R D Hill, D R |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/3538920$$D View this record in MEDLINE/PubMed |
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CitedBy_id | crossref_primary_10_1007_s00436_010_1800_7 crossref_primary_10_1016_S0887_2333_99_00068_5 crossref_primary_10_1093_jac_dkh388 crossref_primary_10_1016_0020_7519_93_90177_Z crossref_primary_10_1007_s00436_009_1363_7 crossref_primary_10_1016_j_exppara_2011_01_011 crossref_primary_10_1128_AAC_43_12_2939 crossref_primary_10_1016_S0001_706X_03_00196_7 crossref_primary_10_1016_S0887_2333_01_00026_1 crossref_primary_10_1128_CMR_14_1_114_128_2001 crossref_primary_10_1007_s00436_012_3035_2 |
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Snippet | A simple, quantitative method has been developed to assess the viability of Giardia lamblia trophozoites after exposure to a potentially lethal agent. This... |
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SubjectTerms | Giardia - growth & development Giardia - physiology Giardiasis - blood Giardiasis - immunology Giardiasis - parasitology Microbiological Techniques |
Title | Giardia lamblia: a culture method for determining parasite viability |
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