Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

• Published in: International journal of environmental research and public health Vol. 17; no. 4; p. 1412
• Main Authors: Jamil, Tariq, Melzer, Falk, Saqib, Muhammad, Shahzad, Asim, Khan Kasi, Khushal, Hammad Hussain, Muhammad, Rashid, Imaad, Tahir, Usman, Khan, Iahtasham, Haleem Tayyab, Muhammad, Ullah, Sami, Mohsin, Mashkoor, Mansoor, Muhammad Khalid, Schwarz, Stefan, Neubauer, Heinrich
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.02.2020; MDPI
• Subjects: Abortion; Agglutination; Agriculture; Animals; Antibodies, Bacterial; Brucellosis; Brucellosis, Bovine - diagnosis; Brucellosis, Bovine - epidemiology; Cattle; Confidence intervals; Deoxyribonucleic acid; DNA; Enzyme-Linked Immunosorbent Assay; Enzymes; Epidemiology; Etiology; Farms; Female; Females; Fetuses; Food contamination & poisoning; Immunization; Infections; Livestock; Livestock farming; Male; Males; Membranes; Milk; Pakistan - epidemiology; Polymerase chain reaction; Pregnancy; Risk analysis; Risk Factors; Seroepidemiologic Studies; Serology; Statistical analysis; Statistical methods; Variables; Pakistan; Punjab Pakistan; Lahore Pakistan; France; United States > US; Pakistan; Brucella abortus; bovine brucellosis; zoonosis

Bovine brucellosis remains a persistent infection in ruminants in Pakistan. A total of 828 (409 buffaloes and 419 cattle) sera were collected from 11 institutional-owned livestock farms in Punjab, Pakistan. The samples were tested by rose bengal plate agglutination test (RBPT) and indirect enzyme-li...