Protective effects of allopurinol against acute liver damage and cirrhosis induced by carbon tetrachloride: Modulation of NF-κB, cytokine production and oxidative stress

• Published in: Biochimica et biophysica acta Vol. 1820; no. 2; pp. 65 - 75
• Main Authors: Aldaba-Muruato, Liseth R., Moreno, Mario G., Shibayama, Mineko, Tsutsumi, Víctor, Muriel, Pablo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2012
• Subjects: alanine transaminase; Alanine Transaminase - blood; Allopurinol; Allopurinol - pharmacology; Animals; antioxidants; Blotting, Western; Carbon Tetrachloride; Cell Extracts; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Chronic Disease; cytokines; Cytokines - biosynthesis; fibrosis; gamma-Glutamyltransferase - metabolism; glutathione; Glutathione - blood; GSH; hydroxyproline; lipid peroxidation; Lipid Peroxidation - drug effects; liver; Liver - drug effects; Liver - enzymology; Liver - pathology; Liver Cirrhosis - blood; Liver Cirrhosis - chemically induced; Liver Cirrhosis - enzymology; Liver Cirrhosis - pathology; liver damage; liver diseases; Male; Matrix Metalloproteinase 13 - metabolism; necrosis; NF-kappa B - metabolism; NF-κB; oxidative stress; Oxidative Stress - drug effects; poisoning; Protective Agents - pharmacology; protective effect; Rats; Rats, Wistar; transcription factor NF-kappa B; transforming growth factor beta; tumor necrosis factor-alpha; Western blotting; xanthine oxidase; Xanthine Oxidase - metabolism; NF-κB; cytokines; carbon tetrachloride; liver damage; Allopurinol; oxidative stress

The aim of this work was to evaluate the hepatoprotective ability of allopurinol to prevent the liver injury induced by carbon tetrachloride (CCl 4). Acute liver damage was induced with CCl 4 (4 g/kg, by gavage); allopurinol (50 mg/kg, by gavage) was given 1 h before and 1 h after CCl 4 intoxication...