Hb Youngstown [β101(G3)Glu → Ala; HBB: c.305A > C]: An Unstable Hemoglobin Variant Causing Severe Hemolytic Anemia

• Published in: Hemoglobin Vol. 38; no. 6; pp. 381 - 384
• Main Authors: Edward, Heather L., Pisani, Louis Almero Du, Rodriguez-Romero, Walter E., Chaves-Villalobos, Jorge, Garcia-Quesada, Jonielle, Harris, Neil S., Luo, Hong-Yuan, Steinberg, Martin H., Forget, Bernard G., Chui, David H.K.
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare USA, Inc 01.12.2014; Taylor & Francis
• Subjects: Adult; Amino Acid Substitution; Anemia, Hemolytic, Congenital - diagnosis; Anemia, Hemolytic, Congenital - genetics; Anemia, Hemolytic, Congenital - pathology; Child; De novo mutation; Female; Hb Youngstown; Hemoglobins, Abnormal - genetics; hemolytic anemia; Humans; Mutation, Missense; Protein Stability; unstable hemoglobin (Hb); Hb Youngstown; unstable hemoglobin (Hb); De novo mutation; hemolytic anemia
• ISSN: 0363-0269; 1532-432X
• DOI: 10.3109/03630269.2014.971960

Abstract Hb Youngstown is a rare hemoglobin (Hb) variant caused by substitution of glutamic acid with alanine at amino acid residue 101 of the β-globin chain as a result of an A > C transversion on the β-globin gene nucleotide sequences [β101(G3)Glu → Ala; HBB: c.305A > C]. We now report three patients from two different families, one from South Africa and the other from Costa Rica, who are heterozygous for this Hb variant. All three carriers had marked hemolysis, consistent with Hb Youngstown being a highly unstable variant. The substitution of glutamic acid, a large and negatively charged amino acid, with alanine, a small and non polar amino acid, in the interface of the α1- and β2-globin subunits might interfere with the transition between the oxy- and deoxyHb, and lead to Hb instability and hemolytic anemia.