Human neutrophil adhesion to bovine aortic endothelium. Evidence for endothelial lipoxygenase activity
We examined the effect of phorbol myristate acetate on cultured bovine aortic endothelial cells to determine the role of endothelial cells in neutrophil‐endothelial cell adhesive interactions. Confluent endothelial cells were preincubated with phorbol myristate acetate and other inflammatory signals...
Saved in:
Published in | Journal of leukocyte biology Vol. 53; no. 6; pp. 619 - 629 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
Society for Leukocyte Biology
01.06.1993
Federation of American Societies for Experimental Biology |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | We examined the effect of phorbol myristate acetate on cultured bovine aortic endothelial cells to determine the role of endothelial cells in neutrophil‐endothelial cell adhesive interactions. Confluent endothelial cells were preincubated with phorbol myristate acetate and other inflammatory signals including JV‐formylmethionyl‐ leucyl‐phenylalanine (f‐Met‐Leu‐Phe), the ionophore A23187, and thrombin; washed extensively; and incubated with 51Cr‐labeled neutrophils. Preincubation of endothelium with A23187, phorbol ester, or thrombin increased adherence of neutrophils by 3.1‐, 5.7‐, and 3.7‐fold over baseline. In contrast, f‐Met‐Leu‐Phe preincubation failed to increase adhesion over baseline. Supernatants from endothelium preincubated with phorbol failed to augment adherence of untreated endothelial cells. Preincubation of endothelium with lipoxygenase inhibitors nordihydroguaiaretic acid (50μM), 5,8,11,14‐eicosatetraenoic acid (50 μM), and BW755C (50 μM) inhibited the effect of phorbol preincubation of endothelium significantly by 55, 27, and 22%, respectively. In contrast, inhibitors of cyclooxygenase and thromboxane synthase or thromboxane receptor antagonists had no effect on phorbol‐induced adhesion. Specific desensitization of neutrophil adhesion to phorbol‐treated endothelium could be demonstrated by prior exposure of neutrophils to low concentrations of leukotriene B4 (3.8 × 10−10 M). Endothelium preincubated with phorbol but not f‐Met‐Leu‐Phe or thrombin produced several fatty acid peaks at 280 nm, one of which comigrated with authentic leukotriene B4 (LTB4). This peak, isolated and purified, increased endothelial cell adherence in a temporal fashion in the same way as LTB4 and was demonstrated to be LTB4 by ultraviolet spectroscopy, high‐ performance liquid chromatography, and mass spectroscopy. These data demonstrate that endothelial cell‐derived lipoxygenase metabolites, in particular LTB4, are involved, in part, in the acute regulation of neutrophil adhesion to endothelium induced by inflammatory signals such as phorbol ester. |
---|---|
AbstractList | We examined the effect of phorbol myristate acetate on cultured bovine aortic endothelial cells to determine the role of endothelial cells in neutrophil-endothelial cell adhesive interactions. Confluent endothelial cells were preincubated with phorbol myristate acetate and other inflammatory signals including N-formylmethionyl-leucyl-phenylalanine (f-Met-Leu-Phe), the ionophore A23187, and thrombin; washed extensively; and incubated with 51Cr-labeled neutrophils. Preincubation of endothelium with A23187, phorbol ester, or thrombin increased adherence of neutrophils by 3.1-, 5.7-, and 3.7-fold over baseline. In contrast, f-Met-Leu-Phe preincubation failed to increase adhesion over baseline. Supernatants from endothelium preincubated with phorbol failed to augment adherence of untreated endothelial cells. Preincubation of endothelium with lipoxygenase inhibitors nordihydroguaiaretic acid (50 microM), 5,8,11,14-eicosatetraenoic acid (50 microM), and BW755C (50 microM) inhibited the effect of phorbol preincubation of endothelium significantly by 55, 27, and 22%, respectively. In contrast, inhibitors of cyclooxygenase and thromboxane synthase or thromboxane receptor antagonists had no effect on phorbol-induced adhesion. Specific desensitization of neutrophil adhesion to phorbol-treated endothelium could be demonstrated by prior exposure of neutrophils to low concentrations of leukotriene B4 (3.8 x 10(-10) M). Endothelium preincubated with phorbol but not f-Met-Leu-Phe or thrombin produced several fatty acid peaks at 280 nm, one of which comigrated with authentic leukotriene B4 (LTB4). This peak, isolated and purified, increased endothelial cell adherence in a temporal fashion in the same way as LTB4 and was demonstrated to be LTB4 by ultraviolet spectroscopy, high-performance liquid chromatography, and mass spectroscopy. These data demonstrate that endothelial cell-derived lipoxygenase metabolites, in particular LTB4, are involved, in part, in the acute regulation of neutrophil adhesion to endothelium induced by inflammatory signals such as phorbol ester. We examined the effect of phorbol myristate acetate on cultured bovine aortic endothelial cells to determine the role of endothelial cells in neutrophil‐endothelial cell adhesive interactions. Confluent endothelial cells were preincubated with phorbol myristate acetate and other inflammatory signals including JV‐formylmethionyl‐ leucyl‐phenylalanine (f‐Met‐Leu‐Phe), the ionophore A23187, and thrombin; washed extensively; and incubated with 51Cr‐labeled neutrophils. Preincubation of endothelium with A23187, phorbol ester, or thrombin increased adherence of neutrophils by 3.1‐, 5.7‐, and 3.7‐fold over baseline. In contrast, f‐Met‐Leu‐Phe preincubation failed to increase adhesion over baseline. Supernatants from endothelium preincubated with phorbol failed to augment adherence of untreated endothelial cells. Preincubation of endothelium with lipoxygenase inhibitors nordihydroguaiaretic acid (50μM), 5,8,11,14‐eicosatetraenoic acid (50 μM), and BW755C (50 μM) inhibited the effect of phorbol preincubation of endothelium significantly by 55, 27, and 22%, respectively. In contrast, inhibitors of cyclooxygenase and thromboxane synthase or thromboxane receptor antagonists had no effect on phorbol‐induced adhesion. Specific desensitization of neutrophil adhesion to phorbol‐treated endothelium could be demonstrated by prior exposure of neutrophils to low concentrations of leukotriene B4 (3.8 × 10−10 M). Endothelium preincubated with phorbol but not f‐Met‐Leu‐Phe or thrombin produced several fatty acid peaks at 280 nm, one of which comigrated with authentic leukotriene B4 (LTB4). This peak, isolated and purified, increased endothelial cell adherence in a temporal fashion in the same way as LTB4 and was demonstrated to be LTB4 by ultraviolet spectroscopy, high‐ performance liquid chromatography, and mass spectroscopy. These data demonstrate that endothelial cell‐derived lipoxygenase metabolites, in particular LTB4, are involved, in part, in the acute regulation of neutrophil adhesion to endothelium induced by inflammatory signals such as phorbol ester. Abstract We examined the effect of phorbol myristate acetate on cultured bovine aortic endothelial cells to determine the role of endothelial cells in neutrophil-endothelial cell adhesive interactions. Confluent endothelial cells were preincubated with phorbol myristate acetate and other inflammatory signals including JV-formylmethionyl- leucyl-phenylalanine (f-Met-Leu-Phe), the ionophore A23187, and thrombin; washed extensively; and incubated with 51Cr-labeled neutrophils. Preincubation of endothelium with A23187, phorbol ester, or thrombin increased adherence of neutrophils by 3.1-, 5.7-, and 3.7-fold over baseline. In contrast, f-Met-Leu-Phe preincubation failed to increase adhesion over baseline. Supernatants from endothelium preincubated with phorbol failed to augment adherence of untreated endothelial cells. Preincubation of endothelium with lipoxygenase inhibitors nordihydroguaiaretic acid (50μM), 5,8,11,14-eicosatetraenoic acid (50 μM), and BW755C (50 μM) inhibited the effect of phorbol preincubation of endothelium significantly by 55, 27, and 22%, respectively. In contrast, inhibitors of cyclooxygenase and thromboxane synthase or thromboxane receptor antagonists had no effect on phorbol-induced adhesion. Specific desensitization of neutrophil adhesion to phorbol-treated endothelium could be demonstrated by prior exposure of neutrophils to low concentrations of leukotriene B4 (3.8 × 10−10 M). Endothelium preincubated with phorbol but not f-Met-Leu-Phe or thrombin produced several fatty acid peaks at 280 nm, one of which comigrated with authentic leukotriene B4 (LTB4). This peak, isolated and purified, increased endothelial cell adherence in a temporal fashion in the same way as LTB4 and was demonstrated to be LTB4 by ultraviolet spectroscopy, high- performance liquid chromatography, and mass spectroscopy. These data demonstrate that endothelial cell-derived lipoxygenase metabolites, in particular LTB4, are involved, in part, in the acute regulation of neutrophil adhesion to endothelium induced by inflammatory signals such as phorbol ester. J. Leukoc. Biol. 53: 619–629; 1993. We examined the effect of phorbol myristate acetate on cultured bovine aortic endothelial cells to determine the role of endothelial cells in neutrophil-endothelial cell adhesive interactions. Confluent endothelial cells were preincubated with phorbol myristate acetate and other inflammatory signals including N-formylmethionyl-leucyl-phenylalanine (f-Met-Leu-Phe), the ionophore A23187, and thrombin; washed extensively; and incubated with super(51)Cr-labeled neutrophils. The data demonstrate that endothelial cell-derived lipoxygenase metabolites, in particular LTB sub(4), are involved, in part, in the acute regulation of neutrophil adhesion to endothelium induced by inflammatory signals such as phorbol ester. |
Author | P J Spagnuolo B Damtew G Goldsmith K Y Tserng |
Author_xml | – sequence: 1 givenname: Belai surname: Damtew fullname: Damtew, Belai – sequence: 2 givenname: George surname: Goldsmith fullname: Goldsmith, George – sequence: 3 givenname: Koy‐Yi surname: Tserng fullname: Tserng, Koy‐Yi – sequence: 4 givenname: Philip J. surname: Spagnuolo fullname: Spagnuolo, Philip J. |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4865591$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/8391052$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kL2P1DAUxC106Ng76GiRXAAVCXbseOMSTvcBWokGastxXi4-OfZiJxv2v8enrJaO6hXzm5mnuUIXPnhA6C0lJSWk-vzk2rJmpSgFlS_QhkrWFExs2QXakC2nRc0JeYWuUnoihLBKkEt02TBJSV1tUP8wj9pjD_MUw36wDutugGSDx1PAbThYD1iHOFmDwXdhGsDZeSzx7cF24A3gPsR_inbY2X34c3wEr1N2mske7HR8jV722iV4c7rX6Nfd7c-bh2L34_7bzZddYThtZEEbzolsWC96IXXVtJTXRjeGMkq2WnSmBq6harkmlHRUgmZVBijwrjMV4ewafVxz9zH8niFNarTJgHPaQ5iTokKKbKUZ_LSCJoaUIvRqH-2o41FRop5nVXlWVTMlVJ414-9OuXM7QneGTztm_f1J18lo10ftjU1njDeiruVzK12xxTo4_rdSfd99JWv1h9Uz2MdhsRFUGrVz-ZFKLctyfvEvjTqflA |
CODEN | JLBIE7 |
CitedBy_id | crossref_primary_10_1002_1097_0169_200006_46_2_137__AID_CM6_3_0_CO_2_8 crossref_primary_10_1161_CIRCRESAHA_116_309492 crossref_primary_10_1016_j_bbalip_2021_158987 crossref_primary_10_1016_0952_3278_95_90133_7 |
ContentType | Journal Article |
Copyright | 1993 Society for Leukocyte Biology 1993 INIST-CNRS |
Copyright_xml | – notice: 1993 Society for Leukocyte Biology – notice: 1993 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7T5 H94 |
DOI | 10.1002/jlb.53.6.619 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Immunology Abstracts AIDS and Cancer Research Abstracts |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef AIDS and Cancer Research Abstracts Immunology Abstracts |
DatabaseTitleList | MEDLINE CrossRef AIDS and Cancer Research Abstracts |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology Biology |
EISSN | 1938-3673 |
EndPage | 629 |
ExternalDocumentID | 10_1002_jlb_53_6_619 8391052 4865591 JLB0619 www53_6_619 |
Genre | article Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | - 0VX 1OB 1OC 2WC 4.4 53G 5GY 5RE ABEFU ABFLS ACGFS ACPRK ACXQS ADACO ADZMN AENEX AFFNX AFRAH ALMA_UNASSIGNED_HOLDINGS BQCPF CS3 DU5 E3Z EBS EJD F5P GJ GX1 H13 HZ K-O L7B OK1 P2P RHF RHI SAMSI SJN TSL VH1 WOQ ZA5 ZGI ZXP --- .GJ 0R~ 18M 29K 33P 5WD AABZA AACZT AAHHS AAPGJ AAPXW AARHZ AASGY AAUAY AAVAP AAWDT AAXRX AAZKR ABCUV ABJNI ABLJU ABMNT ABNHQ ABPQP ABPTD ABWST ABXVV ACAHQ ACCFJ ACCZN ACFRR ACGFO ACPOU ACUTJ ACXBN ACZBC ADBBV ADIPN ADKYN ADOZA ADQBN ADVEK ADXAS AEEZP AEQDE AFGWE AFYAG AGMDO AGQXC AI. AIURR AIWBW AJAOE AJBDE AJEEA ALUQN AMYDB ANFBD APJGH ATGXG AVNTJ BCRHZ C45 D-I DCZOG DRFUL DRSTM EMOBN F9R HZ~ KOP LATKE LEEKS LUTES LYRES O9- OBOKY OCZFY OJZSN OPAEJ OVD OWPYF P2W RJQFR ROL ROX SUPJJ TCN TEORI TMA TR2 W8F WOHZO YHG ZZTAW AAJUZ ABCVL ABXZS ADDAD AEUQT ALXQX BFHJK IQODW OBFPC CGR CUY CVF ECM EIF NPM AAYXX CITATION 7T5 H94 |
ID | FETCH-LOGICAL-c4189-18440983f6f69a28b145ca8c13107a6dc5e4ae2b4a010d19ea325ca1e4ddc2043 |
ISSN | 0741-5400 |
IngestDate | Fri Aug 16 23:23:19 EDT 2024 Fri Aug 23 02:00:18 EDT 2024 Sat Sep 28 08:40:11 EDT 2024 Sun Oct 29 17:10:30 EDT 2023 Sat Aug 24 01:18:33 EDT 2024 Tue Jan 05 20:16:31 EST 2021 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 6 |
Keywords | Human Endothelial cell Bovine Enzyme Arachidonic acid derivatives Cell cell interaction Adhesion Endothelium Vertebrata Mammalia Enzymatic activity Leukotriene B4 Aorta Artiodactyla Neutrophil Lipoxygenase Ungulata |
Language | English |
License | CC BY 4.0 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c4189-18440983f6f69a28b145ca8c13107a6dc5e4ae2b4a010d19ea325ca1e4ddc2043 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
PMID | 8391052 |
PQID | 16960101 |
PQPubID | 23462 |
PageCount | 11 |
ParticipantIDs | proquest_miscellaneous_16960101 crossref_primary_10_1002_jlb_53_6_619 pubmed_primary_8391052 pascalfrancis_primary_4865591 wiley_primary_10_1002_jlb_53_6_619_JLB0619 highwire_smallpub2_www53_6_619 |
ProviderPackageCode | RHF RHI |
PublicationCentury | 1900 |
PublicationDate | June 1993 |
PublicationDateYYYYMMDD | 1993-06-01 |
PublicationDate_xml | – month: 06 year: 1993 text: June 1993 |
PublicationDecade | 1990 |
PublicationPlace | Bethesda, MD |
PublicationPlace_xml | – name: Bethesda, MD – name: United States |
PublicationTitle | Journal of leukocyte biology |
PublicationTitleAlternate | J Leukoc Biol |
PublicationYear | 1993 |
Publisher | Society for Leukocyte Biology Federation of American Societies for Experimental Biology |
Publisher_xml | – name: Society for Leukocyte Biology – name: Federation of American Societies for Experimental Biology |
SSID | ssj0003260 |
Score | 1.491178 |
Snippet | We examined the effect of phorbol myristate acetate on cultured bovine aortic endothelial cells to determine the role of endothelial cells in... Abstract We examined the effect of phorbol myristate acetate on cultured bovine aortic endothelial cells to determine the role of endothelial cells in... |
SourceID | proquest crossref pubmed pascalfrancis wiley highwire |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 619 |
SubjectTerms | Animals Arachidonic Acid - metabolism Biological and medical sciences Cattle Cell Adhesion Cell interactions, adhesion Cells, Cultured endothelium Endothelium, Vascular - enzymology Endothelium, Vascular - physiology Fundamental and applied biological sciences. Psychology Humans Inflammation - pathology Leukotriene B4 - physiology lipoxygenase Lipoxygenase - metabolism Molecular and cellular biology N-Formylmethionine Leucyl-Phenylalanine - pharmacology neutrophil Neutrophils - physiology Tetradecanoylphorbol Acetate - pharmacology Thrombin - pharmacology |
Title | Human neutrophil adhesion to bovine aortic endothelium. Evidence for endothelial lipoxygenase activity |
URI | http://www.jleukbio.org/content/53/6/619.abstract https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fjlb.53.6.619 https://www.ncbi.nlm.nih.gov/pubmed/8391052 https://search.proquest.com/docview/16960101 |
Volume | 53 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwELeqoUl7QbAxUdiGH4AHopR8OG78uCHoVA1e6KTyFDmxq3WkSbUm6sp_xH_JOY6TdIzPl6hKHOea3y_n8_nujNDL0Ocwr3B8WzoOtwkPmc08Htic-mDNh8kwqXJhPn6i55dkPA2mvd73TtRSWcSD5Nu9eSX_gyqcA1xVluw_INt0CifgN-ALR0AYjn-FsfbAZ7IsbvLl1Ty1uLiSyv2lLMpY-QqkxXN1nyUzoXKt0nm5GFhmK1Fd79tcAbDS-TK_3cDzYGyrymyonSV-Yb-msvyaJ5tCWnUhp2ZmD1RaFO2Cw8ga5alYKRdOo96tL9YEXlE9bCrVbI2tz6DcshL6al0ROvCPdl0R3TjTi0aGs44MWq-BEaPiMfRyjNR6l4He9ane1cQoZl1FuCZgV8tSrWV_0v66mux1Gg8Cf0AHd5oBdstFxQQwCsGs9Noh0Cz73xkZm3jF9Xod-BGNqCow-8AbskDN80fTNpoIrGBHV3zV_63OsQCJ3nbl2UO79cO37SBTm1qF5vIVfJ0zva3KffOe7WlUZQdNHqGHNQHwqWbjY9ST2T46OM14kS82-DWuQoorLPbRbo3KAZpVVMUtVbGhKi5yrKmKNVVxl6rYUBUD3LhDVdylKjZUfYIuP7yfvDu36y0-7IS4oBfckBCHhf6MzijjXhi7JEh4mLgw6xhyKpJAEi69mHDHdYTLJPc9aOBKIkSi0roP0U6WZ_IpwjB0J9QTInTITHXC4zgkVYlDJgSncR-9Mi88WupKLpGu2e1FAFFkAO6jE4NGtFrwNIW37kUdBvTR8RZGTW9EJXkzt49eGMwi0NVqAY5nMi9XkUuZ8n9Ai0MNZXNrTYo-elNB-1sBo_HFGVjh7NmfBH2O9tqv9AjtFDelPAYTuohPKvL-AFJSys8 |
link.rule.ids | 315,786,790,27957,27958 |
linkProvider | Geneva Foundation for Medical Education and Research |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Human+neutrophil+adhesion+to+bovine+aortic+endothelium.+Evidence+for+endothelial+lipoxygenase+activity&rft.jtitle=Journal+of+leukocyte+biology&rft.au=B+Damtew&rft.au=G+Goldsmith&rft.au=K+Y+Tserng&rft.au=P+J+Spagnuolo&rft.date=1993-06-01&rft.pub=Society+for+Leukocyte+Biology&rft.issn=0741-5400&rft.eissn=1938-3673&rft.volume=53&rft.issue=6&rft.spage=619&rft_id=info:doi/10.1002%2Fjlb.53.6.619&rft_id=info%3Apmid%2F8391052&rft.externalDBID=n%2Fa&rft.externalDocID=www53_6_619 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0741-5400&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0741-5400&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0741-5400&client=summon |