Spontaneous Cell Lysis by Pelomonas saccharophila MRB3 Provides Plant-Available Macronutrients in Hydroponic Growth Media and Accelerates Biomass Production of Duckweed

This study reports an intriguing observation regarding the betaproteobacterium Pelomonas saccharophila MRB3 (NITE P-01647), a candidate plant growth-promoting bacterium that accelerates biomass production of the aquatic plant, duckweed. In a series of experiments in which strain MRB3 cells were inoc...

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Published inJournal of Water and Environment Technology Vol. 21; no. 1; pp. 49 - 58
Main Authors Ishizawa, Hidehiro, Kaji, Yukiko, Shimizu, Yuki, Kuroda, Masashi, Inoue, Daisuke, Makino, Ayaka, Nakai, Ryosuke, Tamaki, Hideyuki, Morikawa, Masaaki, Ike, Michihiko
Format Journal Article
LanguageEnglish
Published Tokyo Japan Society on Water Environment 2023
Japan Science and Technology Agency
Subjects
Aquatic plants
Bacteria
bacterial autolysis
Biomass
Cell culture
Cell density
Cells
Culture media
Duckweed
Floating plants
Freshwater plants
Growth media
Hydroponics
Inoculation
Lysis
Nitrogen
Nucleic acids
Nutrient dynamics
Plant growth
plant growth-promoting bacteria
Plants
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Abstract This study reports an intriguing observation regarding the betaproteobacterium Pelomonas saccharophila MRB3 (NITE P-01647), a candidate plant growth-promoting bacterium that accelerates biomass production of the aquatic plant, duckweed. In a series of experiments in which strain MRB3 cells were inoculated into duckweed cultures, MRB3 improved duckweed growth with decreasing cell density in the culture medium. By monitoring nutrient dynamics of plants and bacteria, we found that MRB3 exhibited spontaneous cell lysis and released NH4+ and PO43–, which were subsequently utilized for duckweed growth. Surprisingly, the amount of NH4+ released by MRB3 corresponded to approximately half of the total nitrogen contained in the inoculated cells, notwithstanding the fact that the majority of nitrogen elements in bacterial cells should be present in plant-unavailable forms, such as proteins and nucleic acids. Spontaneous cell lysis, provision of macronutrients, and duckweed growth promotion by MRB3 were robustly observed in different inoculation amounts and growth media, including natural pond water. These observations indicated the possibility of utilizing autolytic bacteria as an alternative nutrient source in hydroponic cultivation of duckweeds and other plants.
AbstractList This study reports an intriguing observation regarding the betaproteobacterium Pelomonas saccharophila MRB3 (NITE P-01647), a candidate plant growth-promoting bacterium that accelerates biomass production of the aquatic plant, duckweed. In a series of experiments in which strain MRB3 cells were inoculated into duckweed cultures, MRB3 improved duckweed growth with decreasing cell density in the culture medium. By monitoring nutrient dynamics of plants and bacteria, we found that MRB3 exhibited spontaneous cell lysis and released NH4+ and PO43–, which were subsequently utilized for duckweed growth. Surprisingly, the amount of NH4+ released by MRB3 corresponded to approximately half of the total nitrogen contained in the inoculated cells, notwithstanding the fact that the majority of nitrogen elements in bacterial cells should be present in plant-unavailable forms, such as proteins and nucleic acids. Spontaneous cell lysis, provision of macronutrients, and duckweed growth promotion by MRB3 were robustly observed in different inoculation amounts and growth media, including natural pond water. These observations indicated the possibility of utilizing autolytic bacteria as an alternative nutrient source in hydroponic cultivation of duckweeds and other plants.
ArticleNumber 22-054
Author Ishizawa, Hidehiro
Tamaki, Hideyuki
Nakai, Ryosuke
Kuroda, Masashi
Ike, Michihiko
Makino, Ayaka
Morikawa, Masaaki
Shimizu, Yuki
Kaji, Yukiko
Inoue, Daisuke
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  organization: Graduate School of Engineering, Osaka University, Suita, Japan
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Snippet This study reports an intriguing observation regarding the betaproteobacterium Pelomonas saccharophila MRB3 (NITE P-01647), a candidate plant growth-promoting...
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SubjectTerms Aquatic plants
Bacteria
bacterial autolysis
Biomass
Cell culture
Cell density
Cells
Culture media
Duckweed
Floating plants
Freshwater plants
Growth media
Hydroponics
Inoculation
Lysis
Nitrogen
Nucleic acids
Nutrient dynamics
Plant growth
plant growth-promoting bacteria
Plants
Title Spontaneous Cell Lysis by Pelomonas saccharophila MRB3 Provides Plant-Available Macronutrients in Hydroponic Growth Media and Accelerates Biomass Production of Duckweed
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