Columnar Injection for Intracerebral Cell Therapy

Abstract BACKGROUND Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases continue to develop. The effect of grafting technique on development and survival of the graft has received less attention. Rate and...

Full description

Saved in:
Bibliographic Details
Published inOperative neurosurgery (Hagerstown, Md.) Vol. 18; no. 3; pp. 321 - 328
Main Authors Schweitzer, Jeffrey S, Song, Bin, Leblanc, Pierre R, Feitosa, Melissa, Carter, Bob S, Kim, Kwang-Soo
Format Journal Article
LanguageEnglish
Published United States Oxford University Press 01.03.2020
Wolters Kluwer Health, Inc
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Abstract BACKGROUND Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases continue to develop. The effect of grafting technique on development and survival of the graft has received less attention. Rate and method of graft delivery may impact the cell viability and success of these therapies. Understanding the final location of the graft with respect to the intended target location is also critical. OBJECTIVE To describe a “columnar injection” technique designed to reduce damage to host tissue and result in a column of graft material with greater surface area to volume ratio than traditional injection techniques. METHODS Using a clinically relevant model system of human embryonic stem cell-derived dopaminergic progenitors injected into athymic rat host brain, we describe a novel device that allows separate control of syringe barrel and plunger, permitting precise deposition of the contents into the cannula tract during withdrawal. Controls consist of contralateral injection using traditional techniques. Graft histology was examined at graft maturity. RESULTS Bolus grafts were centered on the injection tract but were largely proximal to the “target” location. These grafts displayed a conspicuous peripheral distribution of cells, particularly of mature dopaminergic neurons. In contrast, column injections remained centered at the intended target, contained more evenly distributed cells, and had significantly more mature dopaminergic neurons. CONCLUSION We suggest that this columnar injection technique may allow better engraftment and development of intracerebral grafts, enhancing outcomes of cell therapy, compared to fixed-point injection techniques.
AbstractList Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases continue to develop. The effect of grafting technique on development and survival of the graft has received less attention. Rate and method of graft delivery may impact the cell viability and success of these therapies. Understanding the final location of the graft with respect to the intended target location is also critical. To describe a "columnar injection" technique designed to reduce damage to host tissue and result in a column of graft material with greater surface area to volume ratio than traditional injection techniques. Using a clinically relevant model system of human embryonic stem cell-derived dopaminergic progenitors injected into athymic rat host brain, we describe a novel device that allows separate control of syringe barrel and plunger, permitting precise deposition of the contents into the cannula tract during withdrawal. Controls consist of contralateral injection using traditional techniques. Graft histology was examined at graft maturity. Bolus grafts were centered on the injection tract but were largely proximal to the "target" location. These grafts displayed a conspicuous peripheral distribution of cells, particularly of mature dopaminergic neurons. In contrast, column injections remained centered at the intended target, contained more evenly distributed cells, and had significantly more mature dopaminergic neurons. We suggest that this columnar injection technique may allow better engraftment and development of intracerebral grafts, enhancing outcomes of cell therapy, compared to fixed-point injection techniques.
Abstract BACKGROUND Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases continue to develop. The effect of grafting technique on development and survival of the graft has received less attention. Rate and method of graft delivery may impact the cell viability and success of these therapies. Understanding the final location of the graft with respect to the intended target location is also critical. OBJECTIVE To describe a “columnar injection” technique designed to reduce damage to host tissue and result in a column of graft material with greater surface area to volume ratio than traditional injection techniques. METHODS Using a clinically relevant model system of human embryonic stem cell-derived dopaminergic progenitors injected into athymic rat host brain, we describe a novel device that allows separate control of syringe barrel and plunger, permitting precise deposition of the contents into the cannula tract during withdrawal. Controls consist of contralateral injection using traditional techniques. Graft histology was examined at graft maturity. RESULTS Bolus grafts were centered on the injection tract but were largely proximal to the “target” location. These grafts displayed a conspicuous peripheral distribution of cells, particularly of mature dopaminergic neurons. In contrast, column injections remained centered at the intended target, contained more evenly distributed cells, and had significantly more mature dopaminergic neurons. CONCLUSION We suggest that this columnar injection technique may allow better engraftment and development of intracerebral grafts, enhancing outcomes of cell therapy, compared to fixed-point injection techniques.
BACKGROUND Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases continue to develop. The effect of grafting technique on development and survival of the graft has received less attention. Rate and method of graft delivery may impact the cell viability and success of these therapies. Understanding the final location of the graft with respect to the intended target location is also critical. OBJECTIVE To describe a “columnar injection” technique designed to reduce damage to host tissue and result in a column of graft material with greater surface area to volume ratio than traditional injection techniques. METHODS Using a clinically relevant model system of human embryonic stem cell-derived dopaminergic progenitors injected into athymic rat host brain, we describe a novel device that allows separate control of syringe barrel and plunger, permitting precise deposition of the contents into the cannula tract during withdrawal. Controls consist of contralateral injection using traditional techniques. Graft histology was examined at graft maturity. RESULTS Bolus grafts were centered on the injection tract but were largely proximal to the “target” location. These grafts displayed a conspicuous peripheral distribution of cells, particularly of mature dopaminergic neurons. In contrast, column injections remained centered at the intended target, contained more evenly distributed cells, and had significantly more mature dopaminergic neurons. CONCLUSION We suggest that this columnar injection technique may allow better engraftment and development of intracerebral grafts, enhancing outcomes of cell therapy, compared to fixed-point injection techniques.
BACKGROUND: Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases continue to develop. The effect of grafting technique on development and survival of the graft has received less attention. Rate and method of graft delivery may impact the cell viability and success of these therapies. Understanding thefinal location of thegraft with respect to the intended target location is also critical. OBJECTIVE: To describe a "columnar injection" technique designed to reduce damage to host tissue and result in a column of graft material with greater surface area to volume ratio than traditional injection techniques. METHODS: Using a clinically relevant model system of human embryonic stem cell-derived dopaminergic progenitors injected into athymic rat host brain, we describe a novel device that allows separate control of syringe barrel and plunger, permitting precise deposition of the contents into the cannula tract during withdrawal. Controls consist of contralateral injection using traditional techniques. Graft histology was examined at graft maturity. RESULTS: Bolus grafts were centered on the injection tract but were largely proximal to the "target" location. These grafts displayed a conspicuous peripheral distribution of cells, particularly of mature dopaminergic neurons. In contrast, column injections remained centered at the intended target, contained more evenly distributed cells, and had significantly more mature dopaminergic neurons. CONCLUSION: We suggest that this columnar injection technique may allow better engraftment and development of intracerebral grafts, enhancing outcomes of cell therapy, compared to fixed-point injection techniques. KEYWORDS: Cell therapy, Dopaminergic neurons, Stereotaxy
BACKGROUNDSurgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases continue to develop. The effect of grafting technique on development and survival of the graft has received less attention. Rate and method of graft delivery may impact the cell viability and success of these therapies. Understanding the final location of the graft with respect to the intended target location is also critical.OBJECTIVETo describe a "columnar injection" technique designed to reduce damage to host tissue and result in a column of graft material with greater surface area to volume ratio than traditional injection techniques.METHODSUsing a clinically relevant model system of human embryonic stem cell-derived dopaminergic progenitors injected into athymic rat host brain, we describe a novel device that allows separate control of syringe barrel and plunger, permitting precise deposition of the contents into the cannula tract during withdrawal. Controls consist of contralateral injection using traditional techniques. Graft histology was examined at graft maturity.RESULTSBolus grafts were centered on the injection tract but were largely proximal to the "target" location. These grafts displayed a conspicuous peripheral distribution of cells, particularly of mature dopaminergic neurons. In contrast, column injections remained centered at the intended target, contained more evenly distributed cells, and had significantly more mature dopaminergic neurons.CONCLUSIONWe suggest that this columnar injection technique may allow better engraftment and development of intracerebral grafts, enhancing outcomes of cell therapy, compared to fixed-point injection techniques.
Audience Academic
Author Song, Bin
Schweitzer, Jeffrey S
Kim, Kwang-Soo
Feitosa, Melissa
Carter, Bob S
Leblanc, Pierre R
AuthorAffiliation 1 Department of Neurosurgery, Massachusetts General Hospital , Boston, Massachusetts
2 Department of Neurosurgery, Harvard Medical School , Boston, Massachusetts
3 Department of Psychiatry, McLean Hospital, Harvard Medical School , Belmont, Massachusetts
4 Molecular Neurobiology Laboratory, Program in Neuroscience, and Harvard Stem Cell Institute, McLean Hospital, Harvard Medical School , Belmont, Massachusetts
AuthorAffiliation_xml – name: 2 Department of Neurosurgery, Harvard Medical School , Boston, Massachusetts
– name: 3 Department of Psychiatry, McLean Hospital, Harvard Medical School , Belmont, Massachusetts
– name: 4 Molecular Neurobiology Laboratory, Program in Neuroscience, and Harvard Stem Cell Institute, McLean Hospital, Harvard Medical School , Belmont, Massachusetts
– name: 1 Department of Neurosurgery, Massachusetts General Hospital , Boston, Massachusetts
Author_xml – sequence: 1
  givenname: Jeffrey S
  surname: Schweitzer
  fullname: Schweitzer, Jeffrey S
  email: JSCHWEITZER1@mgh.harvard.edu
  organization: Department of Neurosurgery, Massachusetts General Hospital, Boston, Massachusetts
– sequence: 2
  givenname: Bin
  surname: Song
  fullname: Song, Bin
  organization: Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts
– sequence: 3
  givenname: Pierre R
  surname: Leblanc
  fullname: Leblanc, Pierre R
  organization: Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts
– sequence: 4
  givenname: Melissa
  surname: Feitosa
  fullname: Feitosa, Melissa
  organization: Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts
– sequence: 5
  givenname: Bob S
  surname: Carter
  fullname: Carter, Bob S
  organization: Department of Neurosurgery, Massachusetts General Hospital, Boston, Massachusetts
– sequence: 6
  givenname: Kwang-Soo
  surname: Kim
  fullname: Kim, Kwang-Soo
  organization: Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts
BackLink https://www.ncbi.nlm.nih.gov/pubmed/31214702$$D View this record in MEDLINE/PubMed
BookMark eNp9kdtKAzEQhoNUtFZvfAApiCBCNZlkTzdCKR4KBW_0OmSzs7plN1mTrlCf3izVoiISyGm--ZmZ_4AMjDVIyDGjl4xm_Moaf2Xbdyb4DhkC5zARENPB9h7BPjnyfkkpZVxENBF7ZJ8zYCKhMCRsZuuuMcqN52aJelVZMy5t_1o5pdFh7lQ9nmFdjx9f0Kl2fUh2S1V7PPo8R-Tp9uZxdj9ZPNzNZ9PFRAuW8klKC8wgj1WuhSpBYIFRhAWogmESIeRYUJ3FQEXJERINWQikRRKBYFqUER-R641u2-UNFhr7imrZuqpRbi2tquTPiKle5LN9kwlnoQAaBM4_BZx97dCvZFN5HTpRBm3nJYDgIs3iNA3o6S90aTtnQnsSBCQgMp59o55VjbIype1n1IvKaZxmnMUs7CNy-QcVVoFNpYN5ZRX-fyRcbBK0s947LLc9Mip7j2XwWG48DvDJ96ls0S9HA3C2AWzX_if0AQEer5Y
CitedBy_id crossref_primary_10_1056_NEJMoa1915872
crossref_primary_10_1227_NEU_0000000000001878
crossref_primary_10_1016_j_arr_2022_101792
crossref_primary_10_1021_acschemneuro_1c00005
crossref_primary_10_3233_JPD_230328
crossref_primary_10_1016_j_pneurobio_2021_102126
Cites_doi 10.3171/jns.1994.81.2.0272
10.1073/pnas.1606342113
10.1093/brain/awm340
10.1056/NEJM200103083441002
10.1002/ana.10359
10.1038/nm1752
10.1007/s13311-011-0082-9
10.3171/jns.2000.92.3.0493
10.1016/j.pneurobio.2018.04.005
10.1073/pnas.1605245113
10.1006/exnr.1999.7198
10.1093/brain/awh510
10.1038/nature23664
10.1002/ana.410380307
10.1177/096368970000900205
10.1016/B978-0-444-59575-1.00004-1
10.1038/nature10648
10.1016/S1474-4422(12)70295-8
10.1038/gt.2008.148
10.1177/096368979500400118
10.1016/S0140-6736(99)90229-5
10.1002/ana.10720
10.1046/j.1471-4159.2003.01931.x
10.1016/0165-3806(88)90027-2
10.1002/ana.22453
10.1016/j.nbd.2005.07.002
10.1016/j.stem.2014.09.017
10.4103/1673-5374.202935
10.1016/j.nbd.2007.08.008
10.1002/cne.24081
10.4103/0976-500X.72351
10.1227/00006123-199505000-00027
10.3233/JPD-140432
ContentType Journal Article
Copyright Copyright © 2019 by the Congress of Neurological Surgeons 2019
Copyright © 2019 by the Congress of Neurological Surgeons.
COPYRIGHT 2020 Oxford University Press
Copyright © 2019 by the Congress of Neurological Surgeons
Copyright_xml – notice: Copyright © 2019 by the Congress of Neurological Surgeons 2019
– notice: Copyright © 2019 by the Congress of Neurological Surgeons.
– notice: COPYRIGHT 2020 Oxford University Press
– notice: Copyright © 2019 by the Congress of Neurological Surgeons
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
3V.
7X7
7XB
8FI
8FJ
8FK
ABUWG
AFKRA
BENPR
CCPQU
FYUFA
GHDGH
K9.
M0S
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
DOI 10.1093/ons/opz143
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central
ProQuest One Community College
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Health & Medical Complete (Alumni)
Health & Medical Collection (Alumni Edition)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
ProQuest One Academic Eastern Edition
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
ProQuest Central China
ProQuest Hospital Collection (Alumni)
ProQuest Central
ProQuest Health & Medical Complete
Health Research Premium Collection
ProQuest One Academic UKI Edition
Health and Medicine Complete (Alumni Edition)
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList MEDLINE

ProQuest One Academic Eastern Edition

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 3
  dbid: 7X7
  name: ProQuest Health and Medical
  url: https://search.proquest.com/healthcomplete
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
EISSN 2332-4260
EndPage 328
ExternalDocumentID A689316189
10_1093_ons_opz143
31214702
10.1093/ons/opz143
Genre Journal Article
Research Support, N.I.H., Extramural
GeographicLocations Massachusetts
California
GeographicLocations_xml – name: Massachusetts
– name: California
GrantInformation_xml – fundername: NIH
  grantid: NS084869; NS070577
  funderid: 10.13039/100000002
– fundername: NINDS NIH HHS
  grantid: R01 NS070577
– fundername: NIH HHS
  grantid: R24 OD024622
– fundername: NINDS NIH HHS
  grantid: R01 NS084869
– fundername: ; ; ;
  grantid: NS084869; NS070577
GroupedDBID 0R~
48X
53G
7X7
8FI
8FJ
AAAAV
AAAXR
AAGIX
AAHPQ
AAIQE
AAPQZ
AAQKA
AARTV
AASCR
AASXQ
AAUQX
AAVAP
ABASU
ABDIG
ABJNI
ABLJU
ABPTD
ABUWG
ABXVJ
ACGFS
ACILI
ACOAL
ACXJB
ACXNZ
ADBBV
ADGZP
ADHKW
ADHPY
ADRTK
AEMDU
AENEX
AENZO
AETBJ
AEWNT
AFDTB
AFFZL
AFKRA
AFOFC
AFUWQ
AGINJ
AHMBA
AHOMT
AHQNM
AJNWD
AJZMW
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AMNEI
APIBT
BAYMD
BCRHZ
BENPR
BPHCQ
BTRTY
BVXVI
BYPQX
C45
CCPQU
CDBKE
DAKXR
EBS
EEVPB
ENERS
ERAAH
EX3
FCALG
FECEO
FLUFQ
FOEOM
FOTVD
FQBLK
FYUFA
GAUVT
GJXCC
HLJTE
HMCUK
IAO
KOP
KSI
MHKGH
NOYVH
NTWIH
O9-
OBH
ODMLO
OVD
PAFKI
PEELM
PQQKQ
PROAC
RLZ
ROX
RUSNO
TEORI
TJX
UKHRP
YAYTL
YKOAZ
YXANX
CGR
CUY
CVF
ECM
EIF
ITC
NPM
1TH
3V.
AAJQQ
AAKAS
AAQOH
AAQQT
AAYXX
ACLDA
ADBIZ
ADZCM
AEETU
AFTRI
AHRYX
AIZYK
CITATION
EJD
H13
IPNFZ
RIG
7XB
8FK
K9.
PQEST
PQUKI
PRINS
7X8
5PM
ID FETCH-LOGICAL-c4183-80de92b6abc4af24ede55ed2ad1e75e2bed0c96204f3e27c29d1e8d75241c4f53
IEDL.DBID 7X7
ISSN 2332-4252
IngestDate Tue Sep 17 20:56:38 EDT 2024
Fri Dec 06 00:17:43 EST 2024
Mon Nov 04 11:29:58 EST 2024
Tue Nov 19 20:49:35 EST 2024
Tue Nov 12 22:41:09 EST 2024
Fri Dec 06 01:58:45 EST 2024
Sat Nov 02 12:20:26 EDT 2024
Wed Aug 28 03:19:53 EDT 2024
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 3
Keywords Dopaminergic neurons
Stereotaxy
Cell therapy
Language English
License This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
Copyright © 2019 by the Congress of Neurological Surgeons.
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c4183-80de92b6abc4af24ede55ed2ad1e75e2bed0c96204f3e27c29d1e8d75241c4f53
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Jeffrey S. Schweitzer and Bin Song contributed equally to this work.
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311830
PMID 31214702
PQID 2427249398
PQPubID 2046367
PageCount 8
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_7311830
proquest_miscellaneous_2243489688
proquest_journals_2427249398
gale_infotracmisc_A689316189
gale_infotracacademiconefile_A689316189
crossref_primary_10_1093_ons_opz143
pubmed_primary_31214702
oup_primary_10_1093_ons_opz143
PublicationCentury 2000
PublicationDate 2020-03-01
PublicationDateYYYYMMDD 2020-03-01
PublicationDate_xml – month: 03
  year: 2020
  text: 2020-03-01
  day: 01
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: Philadelphia
PublicationTitle Operative neurosurgery (Hagerstown, Md.)
PublicationTitleAlternate Oper Neurosurg (Hagerstown)
PublicationYear 2020
Publisher Oxford University Press
Wolters Kluwer Health, Inc
Publisher_xml – name: Oxford University Press
– name: Wolters Kluwer Health, Inc
References Redmond (bib16-20231016) 2008; 29
Brundin (bib12-20231016) 2000; 9
Kikuchi (bib21-20231016) 2017; 548
Lindvall (bib2-20231016) 2004; 1
Olanow (bib9-20231016) 2003; 54
Sonntag (bib5-20231016) 2018; 168
Mendez (bib30-20231016) 2005; 128
Alterman (bib13-20231016) 2011; 70
Freeman (bib17-20231016) 1995; 4
Grealish (bib29-20231016) 2014; 15
Bjorklund (bib3-20231016) 2017; 7
Breeze (bib11-20231016) 1995; 36
Kordower (bib31-20231016) 2016; 113
Emgard (bib37-20231016) 2003; 86
Mendez (bib34-20231016) 2008; 14
Barker (bib8-20231016) 2013; 12
Maries (bib24-20231016) 2006; 21
Kordower (bib25-20231016) 2017; 525
O’Keeffe (bib35-20231016) 2008; 131
Thompson (bib7-20231016) 2012; 200
Brundin (bib10-20231016) 1988; 467
Freed (bib18-20231016) 2011; 8
Ma (bib23-20231016) 2002; 52
Kilkenny (bib28-20231016) 2010; 1
Moore (bib6-20231016) 2014; 4
Freed (bib22-20231016) 2001; 344
Mendez (bib19-20231016) 2000; 92
Backlund (bib1-20231016) 1985; 62
Oh (bib26-20231016) 2009; 16
Emgard (bib33-20231016) 1999; 160
Li (bib36-20231016) 2016; 113
Leigh (bib15-20231016) 1994; 81
Potts (bib14-20231016) 2013; 4
Stoker (bib4-20231016) 2017; 12
Freeman (bib20-20231016) 1995; 38
Kriks (bib27-20231016) 2011; 480
Borlongan (bib32-20231016) 1999; 353
References_xml – volume: 1
  start-page: 382
  issue: 4
  year: 2004
  ident: bib2-20231016
  article-title: Cell therapy in Parkinson disease
  publication-title: NeuroRx J Am Soc Experiment NeuroTher
  contributor:
    fullname: Lindvall
– volume: 81
  start-page: 272
  issue: 2
  year: 1994
  ident: bib15-20231016
  article-title: Vascularization and microvascular permeability in solid versus cell-suspension embryonic neural grafts
  publication-title: J Neurosurg
  doi: 10.3171/jns.1994.81.2.0272
  contributor:
    fullname: Leigh
– volume: 113
  start-page: 6332
  issue: 23
  year: 2016
  ident: bib31-20231016
  article-title: Fetal grafts for Parkinson disease: Decades in the making
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.1606342113
  contributor:
    fullname: Kordower
– volume: 62
  start-page: 1223
  issue: 2
  year: 1985
  ident: bib1-20231016
  article-title: Transplantation of adrenal medullary tissue to striatum in parkinsonism. First clinical trials
  publication-title: Proc Natl Acad Sci USA
  contributor:
    fullname: Backlund
– volume: 131
  start-page: 630
  issue: Pt 3
  year: 2008
  ident: bib35-20231016
  article-title: Induction of A9 dopaminergic neurons from neural stem cells improves motor function in an animal model of Parkinson disease
  publication-title: Brain
  doi: 10.1093/brain/awm340
  contributor:
    fullname: O’Keeffe
– volume: 344
  start-page: 710
  issue: 10
  year: 2001
  ident: bib22-20231016
  article-title: Transplantation of embryonic dopamine neurons for severe Parkinson disease
  publication-title: N Engl J Med
  doi: 10.1056/NEJM200103083441002
  contributor:
    fullname: Freed
– volume: 52
  start-page: 628
  issue: 5
  year: 2002
  ident: bib23-20231016
  article-title: Dyskinesia after fetal cell transplantation for parkinsonism: A PET study
  publication-title: Ann Neurol
  doi: 10.1002/ana.10359
  contributor:
    fullname: Ma
– volume: 14
  start-page: 507
  issue: 5
  year: 2008
  ident: bib34-20231016
  article-title: Dopamine neurons implanted into people with Parkinson disease survive without pathology for 14 years
  publication-title: Nat Med
  doi: 10.1038/nm1752
  contributor:
    fullname: Mendez
– volume: 8
  start-page: 549
  issue: 4
  year: 2011
  ident: bib18-20231016
  article-title: Dopamine cell transplantation for Parkinson disease: The importance of controlled clinical trials
  publication-title: Neurotherapeutics
  doi: 10.1007/s13311-011-0082-9
  contributor:
    fullname: Freed
– volume: 92
  start-page: 493
  issue: 3
  year: 2000
  ident: bib19-20231016
  article-title: Neural transplantation cannula and microinjector system: Experimental and clinical experience. Technical note
  publication-title: J Neurosurg
  doi: 10.3171/jns.2000.92.3.0493
  contributor:
    fullname: Mendez
– volume: 168
  start-page: 1
  issue: Sep
  year: 2018
  ident: bib5-20231016
  article-title: Pluripotent stem cell-based therapy for Parkinson disease: Current status and future prospects
  publication-title: Prog Neurobiol
  doi: 10.1016/j.pneurobio.2018.04.005
  contributor:
    fullname: Sonntag
– volume: 113
  start-page: 6544
  issue: 23
  year: 2016
  ident: bib36-20231016
  article-title: Extensive graft-derived dopaminergic innervation is maintained 24 years after transplantation in the degenerating parkinsonian brain
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.1605245113
  contributor:
    fullname: Li
– volume: 160
  start-page: 279
  issue: 1
  year: 1999
  ident: bib33-20231016
  article-title: Patterns of cell death and dopaminergic neuron survival in intrastriatal nigral grafts
  publication-title: Exp Neurol
  doi: 10.1006/exnr.1999.7198
  contributor:
    fullname: Emgard
– volume: 128
  start-page: 1498
  issue: Pt 7
  year: 2005
  ident: bib30-20231016
  article-title: Cell type analysis of functional fetal dopamine cell suspension transplants in the striatum and substantia nigra of patients with Parkinson disease
  publication-title: Brain
  doi: 10.1093/brain/awh510
  contributor:
    fullname: Mendez
– volume: 548
  start-page: 592
  issue: 7669
  year: 2017
  ident: bib21-20231016
  article-title: Human iPS cell-derived dopaminergic neurons function in a primate Parkinson disease model
  publication-title: Nature
  doi: 10.1038/nature23664
  contributor:
    fullname: Kikuchi
– volume: 38
  start-page: 379
  issue: 3
  year: 1995
  ident: bib20-20231016
  article-title: Bilateral fetal nigral transplantation into the postcommissural putamen in Parkinson disease
  publication-title: Ann Neurol
  doi: 10.1002/ana.410380307
  contributor:
    fullname: Freeman
– volume: 7
  start-page: S23
  issue: s1
  year: 2017
  ident: bib3-20231016
  article-title: Replacing dopamine neurons in parkinson disease: how did it happen?
  publication-title: J Parkinson Dis
  contributor:
    fullname: Bjorklund
– volume: 9
  start-page: 179
  issue: 2
  year: 2000
  ident: bib12-20231016
  article-title: Improving the survival of grafted dopaminergic neurons: A review over current approaches
  publication-title: Cell Transplant
  doi: 10.1177/096368970000900205
  contributor:
    fullname: Brundin
– volume: 200
  start-page: 61
  issue: 200
  year: 2012
  ident: bib7-20231016
  article-title: Survival, differentiation, and connectivity of ventral mesencephalic dopamine neurons following transplantation
  publication-title: Prog Brain Res
  doi: 10.1016/B978-0-444-59575-1.00004-1
  contributor:
    fullname: Thompson
– volume: 480
  start-page: 547
  issue: 7378
  year: 2011
  ident: bib27-20231016
  article-title: Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson disease
  publication-title: Nature
  doi: 10.1038/nature10648
  contributor:
    fullname: Kriks
– volume: 12
  start-page: 84
  issue: 1
  year: 2013
  ident: bib8-20231016
  article-title: Fetal dopaminergic transplantation trials and the future of neural grafting in Parkinson disease
  publication-title: Lancet Neurol
  doi: 10.1016/S1474-4422(12)70295-8
  contributor:
    fullname: Barker
– volume: 16
  start-page: 437
  issue: 3
  year: 2009
  ident: bib26-20231016
  article-title: Expression of transgenes in midbrain dopamine neurons using the tyrosine hydroxylase promoter
  publication-title: Gene Ther
  doi: 10.1038/gt.2008.148
  contributor:
    fullname: Oh
– volume: 4
  start-page: 141
  issue: 1
  year: 1995
  ident: bib17-20231016
  article-title: The influence of donor age on the survival of solid and suspension intraparenchymal human embryonic nigral grafts
  publication-title: Cell Transplant
  doi: 10.1177/096368979500400118
  contributor:
    fullname: Freeman
– volume: 353
  start-page: SI29
  issue: Suppl 1
  year: 1999
  ident: bib32-20231016
  article-title: Neural transplantation for neurodegenerative disorders
  publication-title: Lancet
  doi: 10.1016/S0140-6736(99)90229-5
  contributor:
    fullname: Borlongan
– volume: 54
  start-page: 403
  issue: 3
  year: 2003
  ident: bib9-20231016
  article-title: A double-blind controlled trial of bilateral fetal nigral transplantation in Parkinson disease
  publication-title: Ann Neurol
  doi: 10.1002/ana.10720
  contributor:
    fullname: Olanow
– volume: 86
  start-page: 1223
  issue: 5
  year: 2003
  ident: bib37-20231016
  article-title: Both apoptosis and necrosis occur early after intracerebral grafting of ventral mesencephalic tissue: A role for protease activation
  publication-title: J Neurochem
  doi: 10.1046/j.1471-4159.2003.01931.x
  contributor:
    fullname: Emgard
– volume: 467
  start-page: 233
  issue: 2
  year: 1988
  ident: bib10-20231016
  article-title: Survival and function of dissociated rat dopamine neurones grafted at different developmental stages or after being cultured in vitro
  publication-title: Brain Res
  doi: 10.1016/0165-3806(88)90027-2
  contributor:
    fullname: Brundin
– volume: 4
  start-page: S22
  issue: Suppl 1
  year: 2013
  ident: bib14-20231016
  article-title: Devices for cell transplantation into the central nervous system: Design considerations and emerging technologies
  publication-title: Surg Neurol Int
  contributor:
    fullname: Potts
– volume: 70
  start-page: 5
  issue: 1
  year: 2011
  ident: bib13-20231016
  article-title: Open-label surgical trials for Parkinson disease: Time for reconsideration
  publication-title: Ann Neurol
  doi: 10.1002/ana.22453
  contributor:
    fullname: Alterman
– volume: 21
  start-page: 165
  issue: 1
  year: 2006
  ident: bib24-20231016
  article-title: Focal not widespread grafts induce novel dyskinetic behavior in parkinsonian rats
  publication-title: Neurobiol Dis
  doi: 10.1016/j.nbd.2005.07.002
  contributor:
    fullname: Maries
– volume: 15
  start-page: 653
  issue: 5
  year: 2014
  ident: bib29-20231016
  article-title: Human ESC-derived dopamine neurons show similar preclinical efficacy and potency to fetal neurons when grafted in a rat model of Parkinson disease
  publication-title: Cell stem cell
  doi: 10.1016/j.stem.2014.09.017
  contributor:
    fullname: Grealish
– volume: 12
  start-page: 389
  issue: 3
  year: 2017
  ident: bib4-20231016
  article-title: Neural grafting for Parkinson disease: Challenges and prospects
  publication-title: Neural Regen Res
  doi: 10.4103/1673-5374.202935
  contributor:
    fullname: Stoker
– volume: 29
  start-page: 103
  issue: 1
  year: 2008
  ident: bib16-20231016
  article-title: Influence of cell preparation and target location on the behavioral recovery after striatal transplantation of fetal dopaminergic neurons in a primate model of Parkinson disease
  publication-title: Neurobiol Dis
  doi: 10.1016/j.nbd.2007.08.008
  contributor:
    fullname: Redmond
– volume: 525
  start-page: 498
  issue: 3
  year: 2017
  ident: bib25-20231016
  article-title: Parkinsonian monkeys with prior levodopa-induced dyskinesias followed by fetal dopamine precursor grafts do not display graft-induced dyskinesias
  publication-title: J Comp Neurol
  doi: 10.1002/cne.24081
  contributor:
    fullname: Kordower
– volume: 1
  start-page: 94
  issue: 2
  year: 2010
  ident: bib28-20231016
  article-title: Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research
  publication-title: J Pharmacol Pharmacother
  doi: 10.4103/0976-500X.72351
  contributor:
    fullname: Kilkenny
– volume: 36
  start-page: 1044
  issue: 5
  year: 1995
  ident: bib11-20231016
  article-title: Implantation of fetal tissue for the management of Parkinson disease: a technical note
  publication-title: Neurosurgery
  doi: 10.1227/00006123-199505000-00027
  contributor:
    fullname: Breeze
– volume: 4
  start-page: 671
  issue: 4
  year: 2014
  ident: bib6-20231016
  article-title: Which patients with Parkinson disease participate in clinical trials? One centre's experiences with a new cell based therapy trial (TRANSEURO)
  publication-title: J Parkinson Dis
  doi: 10.3233/JPD-140432
  contributor:
    fullname: Moore
SSID ssj0001345074
Score 2.1966693
Snippet Abstract BACKGROUND Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other...
Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases continue to...
BACKGROUND: Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases...
BACKGROUND Surgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases...
BACKGROUNDSurgical implantation of cellular grafts into the brain is of increasing importance, as stem cell-based therapies for Parkinson and other diseases...
SourceID pubmedcentral
proquest
gale
crossref
pubmed
oup
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 321
SubjectTerms Analysis
Animals
Brain
Cell- and Tissue-Based Therapy
Concepts, Innovations and Techniques
Dopamine
Embryonic stem cells
Humans
Neurons
Neurosurgery
Parkinson's disease
Rats
Stem cells
Surgical techniques
Transplantation
Title Columnar Injection for Intracerebral Cell Therapy
URI https://www.ncbi.nlm.nih.gov/pubmed/31214702
https://www.proquest.com/docview/2427249398
https://search.proquest.com/docview/2243489688
https://pubmed.ncbi.nlm.nih.gov/PMC7311830
Volume 18
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfR3LbtswTNjayy5Dh-7hNQs8bMBORq2XZZ2GLmjRDVgxDC2Qm6EHhW0InCxtL_36kraTxj30aIuQbJLiQ6RIxj4Hx4W34IokdCyUdLqwyaZCRpOULCOqbLo7_POiOr9SP-Z6Phy4XQ9plRuZ2AnquAx0Rn6MqsSgqyBt_XX1v6CuURRdHVpoPGf7XKAqR342c_NwxiIVmjuq6y8nRYHsKTYVSq08psDwcnXXXdjZ0UmDZB5dd9uxOh8nT-5oo7MD9nIwI_OTnu6v2DNoDxmfkahp3Tr_3v7rUqzaHG1SfMIpAqwpRrzIZ7BY5Jd9MYHX7Ors9HJ2XgwtEYqgcPOhPolgha-cD8oloSCC1hCFixyMBuEhlsFSjfkkQZggLA7U0WhU1EElLd-wvXbZwjuWW-lKLzgkqNFH9N5xXiUvXY2vNI8mY582SGlWfeWLpo9YywZR1_Soy9gXwldD24H-xQ1Z_bgGFZZqTio0iKgov83YZASJbBxGw1PE-JMrTTbEaIZ9dt08cEXGPm6HaWrKHWtheYswQklV26pGmLc97bbLSE59mkqRMTOi6haAqm-PR9q_f7oq3EaibybL909_1hF7IchD77LWJmzvZn0LH9CMufHTjlenbP_b6cWv3_f2-PP8
link.rule.ids 230,314,780,784,885,12056,21388,27924,27925,31719,31720,33744,33745,43310,43805
linkProvider ProQuest
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB7B9gAXBOIVupQgkDhFTfxI4hMqq1ZbaFcIbaXeLL-iglbZ7ba98OuZSbzbDYceE4_sZGbsGXvG3wB8dqZgVgWTNUz6THAjM9WoJuO-agTPPZpsujt8PiunF-L7pbyMB243Ma1ysyZ2C7VfOjojP0RTUuFWgav66-o6o6pRFF2NJTQewx4hp8sR7H07nv38dX_KwgU6PKKrMMdZhgrKNhilih9SaHi5-ttd2dmxSnFtHlx42_E7_0-f3LFHJ8_hWXQk06Ne8i_gUWhfQjGhxaY16_S0_dMlWbUpeqX4hF24sKYo8SKdhMUinfdwAq_g4uR4PplmsShC5gROP7QoPihmS2OdMA0TwQcpg2fGF6GSgdngc6cIZb7hgVWOKWyofSXRVDvRSP4aRu2yDW8hVdzklhWhCTXuEq01RVE2lpsaX8nCVwl82jBFr3rsC93HrLlG1umedQl8IX5pmhD0Lybm9eMYBC2lj0p0iQiWXyUwHlCiIrtB8wFy_MGRxhth6DjTbvS9XiTwcdtMXVP2WBuWd0jDBBe1KmukedPLbjsML6hSU84SqAZS3RIQ_vawpf191eFwVxx3Zzx_9_BnfYAn0_n5mT47nf3Yh6eM9utdDtsYRrfru_AenZpbexA19x8N8_a4
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Columnar+Injection+for+Intracerebral+Cell+Therapy&rft.jtitle=Operative+neurosurgery+%28Hagerstown%2C+Md.%29&rft.au=Schweitzer%2C+Jeffrey+S&rft.au=Song%2C+Bin&rft.au=Leblanc%2C+Pierre+R&rft.au=Feitosa%2C+Melissa&rft.date=2020-03-01&rft.pub=Oxford+University+Press&rft.issn=2332-4252&rft.volume=18&rft.issue=3&rft.spage=321&rft_id=info:doi/10.1093%2Fons%2Fopz143&rft.externalDocID=A689316189
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2332-4252&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2332-4252&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2332-4252&client=summon