CCL22 Recruits CD4-positive CD25-positive Regulatory T Cells into Malignant Pleural Effusion

• Published in: Clinical cancer research Vol. 15; no. 7; pp. 2231 - 2237
• Main Authors: Qin, Xue-Jun, Shi, Huan-Zhong, Deng, Jing-Min, Liang, Qiu-Li, Jiang, Jing, Ye, Zhi-Jian
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 01.04.2009
• Subjects: Adult; Aged; Aged, 80 and over; Antineoplastic agents; Biological and medical sciences; Chemokine CCL17 - analysis; Chemokine CCL22 - metabolism; Chemokine CCL22 - pharmacology; Chemokine CCL22 - physiology; Chemotaxis; Humans; Interleukin-2 Receptor alpha Subunit - analysis; lung cancer; malignant pleural effusion; Medical sciences; Middle Aged; Pharmacology. Drug treatments; Pleural Effusion, Malignant - immunology; regulatory T cells; T-Lymphocytes, Regulatory - immunology; CD4 T lymphocyte; T-Lymphocyte; Pleural disease; Malignant tumor; Malignant pleural effusion; Regulatory cell; Cancer
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.CCR-08-2641

Purpose: The aim of this study was to explore the presence of the chemokines CCL22 and CCL17 in malignant pleural effusion, and the chemoattractant activity of these chemokines on CD4-positive CD25-positive Foxp3-positive regulatory T cells infiltrating into the pleural space. Experimental Design: The concentrations of CCL22 and CCL17 in both pleural effusions and sera from 33 patients with lung cancer were determined. Flow cytometry was done to determine T lymphocyte subsets in cell pellets of pleural effusion. Pleural cells were analyzed for the expression of CCL22 and CCL17. The chemoattractant activity of CCL22 for regulatory T cells in vitro and in vivo was also observed. Results: The concentration of CCL22 in malignant pleural effusion was significantly higher than that in the corresponding serum. Pleural fluid from lung cancer patients was chemotactic for regulatory T cells, and this activity was partly blocked by an anti-CCL22, but not by an anti-CCL17 antibody. Intrapleural administration of CCL22 of patients produced a marked progressive influx of regulatory T cells into pleural space. Conclusions: Compared with serum, CCL22 seemed to be increased in malignant pleural effusion, and could directly induce regulatory T cell infiltration into the pleural space in patients with malignant effusion.