Listening to the Whispers in Neuroimmune Crosstalk: A Comprehensive Workflow to Investigate Neurotrophin Receptor p75NTR Under Endogenous, Low Abundance Conditions

• Published in: Frontiers in immunology Vol. 12; p. 648283
• Main Authors: Dorschner, Benjamin W, Wiedemuth, Ralf, Funke, Ann-Christin, Gentzel, Marc, Rogers, Mary-Louise, Brenner, Sebastian, Thieme, Sebastian
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 16.04.2021
• Subjects: CD271 (p75NTR); endogenous immunoprecipitation; Immunology; mass spectrometry; p75NTR; PMDC05; protein-protein interaction; A375 (human melanoma) cell line; PMDC05; p75NTR; CD271 (p75NTR); endogenous immunoprecipitation; mass spectrometry; protein-protein interaction; neuroimmune crosstalk

Inflammatory conditions are critically influenced by neuroimmune crosstalk. Cytokines and neurotrophic factors shape the responses of both nervous and immune systems. Although much progress has been made, most findings to date are based on expression of recombinant (tagged) proteins. The examination...